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人Ⅰ型基质金属蛋白酶基因真核表达重组质粒的构建

Zhongshan Daxue xuebao. Yixue kexue ban, 2001
【目的】构建人Ⅰ 型基质金属蛋白酶基因(MMP1)真核表达重组质粒, 并进行序列分析。【方法】用逆转录聚 合酶链反应扩增人Ⅰ 型基质金属胶原酶cDNA, 获得目的基因片段(1 407 bp)连接至pcDNA3 载体, 并转化大肠杆菌DH5α, 筛 选阳性克隆并鉴定, 并对片断全长进行DNA 序列测定。【结果】所克隆人Ⅰ 型基质金属胶原酶cDNA, 含全长MMP1 编码区, 与GeneBank 公布序列比较, 仅1 318 位的胞苷酸C 突变为腺苷酸A。并成功构建了含有MMP1 ...
彭　慧, 汪　谦, 黄洁夫
doaj  

[ROMO1 Promotes the Repair of Radiation-induced Skin Injury by Regulating Mitochondrial Function and Keratinocyte Proliferation]. [PDF]

Sichuan Da Xue Xue Bao Yi Xue Ban
Yang T, Tu W, Tang X, DU M, Maheliya A, Yu Z, Qu S, Bai H, Zhang S.   +8 more
europepmc   +1 more source

[Diffusion cycle-consistent generative adversarial networks for pelvic active bone marrow segmentation]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Zhuo L, Zeng M, Tan S, Liang T, Xiao W, Zhen X.   +5 more
europepmc   +1 more source

[<i>MUTYH-APEX1</i> Axis Promotes Hepatocellular Carcinoma Progression and Therapeutic Resistance by Regulating Cell Cycle Proteins]. [PDF]

Sichuan Da Xue Xue Bao Yi Xue Ban
Zhou Y, Zhang H, Zhu Y, Jiang P, Feng T, Wu Z, Chen J.   +6 more
europepmc   +1 more source

[Experimental investigation of skin tissue-derived extracellular vesicles isolated from type 2 diabetic foot ulcers in impaired wound healing]. [PDF]

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
Li R, Zhou Y, Chen J, Wang L, Jian X, Qi F, Deng C, Wei Z, Liu Z.   +8 more
europepmc   +1 more source

[Transcriptomic characteristics of cervical endplate cartilage in cervical spondylosis and effects of acupotomy on the FGF18/Akt axis with cervical spondylosis]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Liu F, Khaliunaa T, Cao Q, Yang Y, Ren C, Zhu J, Zhao X, Cao L, Deng B, Wang X.   +9 more
europepmc   +1 more source

[Role of m6A methylation modification in photoaging and ultraviolet-induced skin cancers]. [PDF]

Zhong Nan Da Xue Xue Bao Yi Xue Ban
Hou Y, Zhang X, Liu J, Wu J.
europepmc   +1 more source

[Exploring the research on the molecular mechanisms of autoimmune liver diseases]. [PDF]

Zhonghua Gan Zang Bing Za Zhi
Liu YQ, Jiang XW, Li Y, Liu XL.
europepmc   +1 more source

[The influence of different collagenases on the analysis of immune cells in salivary glands of mice with chronic graft-versus-host disease]. [PDF]

Zhonghua Xue Ye Xue Za Zhi
Hu ZY, Song QX, Yang SJ, Wei J, Zhang X.
europepmc   +1 more source

大鲵蛙病毒编码96L蛋白(ADRV-96L)的腺苷三磷酸酶活性和促进细胞生长作用 [PDF]

, 2018
张奇亚, 张锐
core