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F10基因对细胞中PCNA和Cyclin D1表达的影响

open access: yesZhongshan Daxue xuebao. Yixue kexue ban, 2009
【目的】 确定F10基因表达对细胞生长的影响,明确F10基因的功能。 【方法】 构建F10基因的真核表达载体,转染重组pRc-CMV2-F10质粒于肺癌细胞系A549, 筛选并获得稳定表达F10基因的细胞克隆A549-F10。通过MTT实验和流式细胞检测F10对细胞生长的影响;通过免疫组化检测细胞中增殖核抗原(PCNA)和细胞周期素D1(cyclin D1)的表达。【结果】 转基因的G418抗性克隆能够检测到F10表达。MTT和流式结果显示F10有促进细胞增殖的作用 ...
doaj  

[Granuloma faciale and Takayasu arteritis in a child: a case report]. [PDF]

open access: yesZhongguo Dang Dai Er Ke Za Zhi
Liao W   +5 more
europepmc   +1 more source

[Advances on efficacy and mechanisms of acid fibroblast growth factor for promoting injured tissue repair]. [PDF]

open access: yesZhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
Zhu F   +5 more
europepmc   +1 more source

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