Results 11 to 20 of about 6,707 (160)

Whole genome sequencing and de novo assembly of Staphylococcus pseudintermedius: a pangenome approach to unravelling pathogenesis of canine pyoderma

open access: yesVeterinary Dermatology, Volume 32, Issue 6, Page 654-663, December 2021., 2021
Background Staphylococcus pseudintermedius is the main aetiological agent of canine pyoderma. Whole genome sequencing is the most comprehensive way of obtaining relevant genomic information about micro‐organisms. Hypothesis/Objectives Oxford Nanopore technology enables quality sequencing and de novo assembly of the whole genome of S.
Lluís Ferrer   +13 more
wiley   +1 more source

Clindamycin resistance of skin derived Staphylococcus pseudintermedius is higher in dogs with a history of antimicrobial therapy

open access: yesVeterinary Dermatology, Volume 31, Issue 4, Page 305-e75, August 2020., 2020
Background – In the Netherlands there is a lack of data regarding resistance of Staphylococcus pseudintermedius to the systemic antimicrobial drugs used for the treatment of superficial pyoderma. Objectives – To assess antimicrobial resistance, with emphasis on resistance to clindamycin and meticillin, in clinical isolates of S.
Catharina M.M. van Damme   +3 more
wiley   +1 more source

逆转骨肉瘤耐药的实验研究

open access: yesZhongguo shiyan zhenduanxue, 2021
目的构建骨肉瘤耐药细胞,探讨LIMK1(LIM Kinase1)逆转骨肉瘤耐药的作用及机制。方法通过长春新碱(VCR)诱导建立人成骨肉瘤MG63的耐药细胞亚系,应用半数抑制浓度公式计算IC50值及耐药指数评估细胞的耐药性;CCK-8法检测细胞增殖抑制率;相差倒置显微镜观察耐药细胞细胞形态的改变;PT-PCR实验检测耐药基因MDR1及其相关基因LIMK1的表达;转染pSUPER-LIMK1重组质粒,验证沉默LIMK1基因逆转MG63耐药的作用并分析其机制。结果本研究所构建的耐药细胞的耐药指数为7.01 ...
黄丽红   +4 more
doaj  

Assessment of contamination and drug resistance profiles of non-typhoid Salmonella in pig slaughtering industry in Shandong Province [PDF]

open access: yes, 2023
ObjectiveTo understand the contamination and drug resistance characteristics of non-typhoidal Salmonella (NTS) during pig slaughter.MethodsA total of 170 carcasses and environmental samples were collected from two large-scale pig slaughterhouses in May ...
BAI Li   +6 more
core   +1 more source

血流感染的耐碳青霉烯类鲍曼不动杆菌的分子机制及临床研究 [PDF]

open access: yes, 2018
目的探讨血流感染的耐碳青霉烯类鲍曼不动杆菌(CRAB)耐药机制,分析菌株同源性及患者的临床特征,为CRAB的感染控制提供实验依据。方法采用Bact/Alert 3D全自动血培养系统进行血培养,细菌鉴定和药敏使用Vitek-2 Compact全自动微生物分析系统。对2010年6月—2016年5月临床血流感染患者分离到的27株碳青霉烯类药物(亚胺培南和/或美罗培南MIC≥16μg/m L)耐药的CRAB复苏菌株,重新采用Vitek-MS质谱分析仪鉴定,采用改良Hodge试验检测碳青霉烯酶 ...
徐和平   +6 more
core   +1 more source

Comparative Genomic Analysis of Functional Genomics of Lactiplantibacillus plantarum ST [PDF]

open access: yes, 2023
In this study, the functional genomic characteristics of Lactiplantibilus plantarum ST were analyzed. Comparative genomic analysis was performed on the whole genome sequences of L. plantarum ST and the type strain L.
YANG Shujuan, ZHOU Jinping, LI Haiyan, CAO Zhenhui, SUN Zhihong, LIN Qiuye
core   +1 more source

Contamination and mobile colistin resistance gene analysis for Salmonella isolated from retail chickens in Ji'nan [PDF]

open access: yes, 2023
ObjectiveTo investigate the contamination of Salmonella in retail chickens in Ji’nan city from 2020 to 2021, and to explore the prevalence of the mobile colistin resistance gene (mcr) among these Salmonella isolates.MethodsFrom December 2020 to November ...
HU Yujie   +7 more
core   +1 more source

多重耐药鲍曼不动杆菌相关耐药基因的研究

open access: yesZhongguo shiyan zhenduanxue, 2021
目的 研究院内MDRAB相关耐药基因及分子流行病学特征,为抗菌药物合理使用及医院感染控制提供依据。方法 对2019年2月至2019年10月间院内分离的15株MDRAB用多位点序列分型(MLST)鉴定菌株之间的亲缘关系,PCR法检测β-内酰胺类耐药基因、质粒介导的喹诺酮类耐药基因、氨基糖苷类耐药基因及消毒剂qacE△1基因。结果 MLST分析发现15株MDRAB有ST195(46.7%)、ST369(20%)、ST208(13.3%)、ST368(13.3%)和ST540(6.7%)5个序列类型 ...
姜梅杰   +4 more
doaj  

Infection and Drug Resistance of Mycoplasma Pneumoniae: an Analysis of Influenza-like Illness [PDF]

open access: yes, 2022
BackgroundInfluenza and Mycoplasma pneumonia (MP) infection are common winter diseases in northern China, both of which have similar clinical symptoms.
TU Peng, DOU Haiwei, SHI Dawei, WAN Ruijie, TIAN Xiujun, YUAN Qing, CHEN Xiaohua, XIN Deli
core   +1 more source

International clinical practice recommendations on the definition, diagnosis, assessment, intervention, and psychosocial aspects of developmental coordination disorder – Chinese (Mandarin) translation

open access: yesDevelopmental Medicine &Child Neurology, Volume 61, Issue 3, Page E1-E35, March 2019., 2019
目的 本国际临床指南由欧洲残疾儿童学会(the European Academy of Childhood Disability,EACD)牵头制定,旨在解决发育性协调障碍(developmental coordination disorder,DCD)的定义、诊断、评估、干预以及与社会心理方面的临床应用关键问题。 方法 本指南针对五个领域的关键问题,通过文献综述和专家团队的正式讨论达成共识。为保证指南的循证基础,以“机制”、“评估”和“干预”为检索词, 对2012年更新以来提出的最新建议以及新增的“社会心理问题”和“青少年/成人”为检索词进行检索。根据牛津大学循证医学中心证据等级 (证据水平 [level of evidence, LOE]1–4) 将结果进行分类,最终转化为指南建议。并由国际 ...
Jing Hua   +6 more
wiley   +1 more source

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