Results 41 to 50 of about 6,707 (160)
野生动物学报, 2020 的：为了解黑龙江省和吉林省健康貉中大肠杆菌四环素耐药基因的分布情况，以貉源大肠杆菌为模板，检测四环素耐药基因，为控制耐药性奠定基础，有利于临床合理用药。方法：共选取56株貉大肠杆菌，通过PCR扩增靶基因以检测耐药基因的携带状态。 结果：四环素抗性基因tetM的检出率为82.14%，还检测到tetA、tetB、tetC、tetD，未检测出tetE。结论：大肠杆菌四环素耐药性可能由几种耐药基因共同介导。试验结果可为貉临床实践中合理使用四环素和预防四环素抗性出现提供理论依据。
刘竟雅 +5 more
doaj
Drug resistance gene variation of HIV-1 strains in Huzhou City， Zhejiang Province [PDF]
ObjectiveTo investigate the variation of drug resistance genes in human immunodeficiency virus (HIV)-1 strains in Huzhou City, Zhejiang Province, so as to provide a basis for guiding the adjustment of treatment plans for ADIS patients or patients ...
LU Zhonghao +4 more
core +1 more source
Zhongguo shiyan zhenduanxue, 2013 目的分析氨基糖苷类高水平耐药（High Level Aminoglyeoside Resistant,HLAR）肠球菌的耐药性与耐药基因,并研究其耐药分子机制。方法采用VITEK-2全自动鉴定仪法测定53株氨基糖苷类高水平耐药肠球菌对11种抗菌药物的最小抑菌浓度,用聚合酶链反应（PCR）检测肠球菌转座子Tn917和Tnl546/Tn916、红霉素耐药基因ermB、毒力岛基因mefA、I类整合酶IntI1、氯霉素耐药基cat、表面蛋白基因esp,并对万古霉素耐药肠球菌（Vancomycin ...
徐韫健, 梁权辉, 刘长连
doaj
Epidemiological characteristics and drug resistance analysis of Salmonella I4, [5], 12∶I∶-in Maoming City from 2017 to 2019 [PDF]
, 2020 Objective To understand the epidemic characteristics and drug resistance of Salmonella I4, [5], 12∶I∶-in Maoming City. Methods Salmonella I4, [5], 12∶I∶-were identified by serological method, and then antibiotic sensitivity of isolated Salmonella I4, [5],
Guodong LIAO +3 more
core +1 more source
Zhongguo shiyan zhenduanxue, 2015 目的研究多药耐药鲍曼不动杆菌（MDRAB）的耐药机制,为临床有效治疗该菌感染提供依据。方法应用PCR技术对94株MDRAB进行耐药基因检测,比较8种耐药基因阳性组与阴性组菌株的耐药性。结果耐药基因检出率:VIM 35.1%、SIM 55.0%、TEM 59.6%、OXA-23 93.6%、OXA-24 52.1%、SHV 52.1%、PER-1 83.0%、IMP 2.1%;仅IPM、MEM、SAM在OXA-23基因阳性组与阴性组间,和SAM在SIM基因阳性组与阴性组间的耐药性有显著差异。结论 ...
池细俤 +4 more
doaj
Zhongguo shiyan zhenduanxue, 2011 目的探讨临床分离的多重耐药铜绿假单胞菌耐药性及相关耐药基因。方法采用纸片扩散法进行药敏试验,VITEK 2全自动微生物鉴定及药敏系统对其进行重新鉴定,聚合酶链反应（PCR）扩增oprD2、金属酶基因、氨基糖苷类酶修饰基因。结果 40株铜绿假单胞菌对临床常用的抗生素:厄它培南,亚胺培南（IPM）、头孢他啶（CAZ）、美罗培南、头孢吡肟（PEP）、哌拉西林/他唑巴坦（TZP）、环丙沙星、氨曲南（ATM）、庆大霉素（GEN）、妥布霉素、阿米卡星、多粘菌素耐药率分别为100%、96.2%、92.0%、90.1%、
赵祝香 +4 more
doaj
Zhongguo shiyan zhenduanxue, 2021 目的研究神经重症监护病房(NICU)中肺炎克雷伯病原菌(Klebsiella pneumoniae,KP)的抗菌药物敏感性、相关耐药基因携带情况,及其与临床耐药表型的关系,为KP感染控制、预防和治疗提供科学合理的临床指导。方法收集2015年1月-2018年12月NICU病人分离出来的及病房环境中定植KP,使用K-B法检测其临床抗菌药物敏感性,同步进行超广谱β-内酰胺酶(ESBLs)表型确证试验,聚合酶链式反应检测其染色体和质粒DNA上所携带的β-内酰胺类、喹诺酮类等相关耐药基因 ...
刘妍 +5 more
doaj
Distribution and drug resistance characteristics of Acinetobacter baumannii in the environment of a general hospital in Xuhui District of Shanghai from 2018 to 2023 [PDF]
ObjectiveTo analyze the distribution, drug resistance characteristics, and changing trends of Acinetobacter baumannii (AB) isolated from environmental surfaces and healthcare workers’ hands in a grade Ⅱ level A general hospital in Xuhui District of ...
JIANG Lufang +8 more
core +1 more source
Zhongguo shiyan zhenduanxue, 2013 目的了解临床分离的鲍曼不动杆菌耐药性及β-内酰胺酶耐药基因的存在类型。方法用美国BD公司Phoenix-100型全自动细菌鉴定药敏系统鉴定细菌及做药敏试验,用聚合酶链反应（PCR）方法检测5种常见β-内酰胺酶耐药基因。采用随机引物PCR扩增对菌株进行分型。结果 50株鲍曼不动杆菌对多粘菌素B全部敏感,,而对其余18种抗生素耐药率为52%-100%,其中24株为泛耐药菌株,共分为8种基因型,B型菌株为主要的流行株,所有菌株均检出TEM-1基因,46株检出AmpC基因,31株检出0XA-24基因 ...
邓丽华 +4 more
doaj
Etiological characteristics of two strains of human-origin Escherichia albertii in Baoshan District， Shanghai [PDF]
ObjectiveTo analyze the etiological characteristics of two strains of Escherichia albertii first identified in Baoshan District, Shanghai, in 2024, to explore their antibiotic resistance characteristics, virulence characteristics, and multilocus sequence
JIN Min +3 more
core +1 more source