Epidemiological characteristics and drug resistance analysis of Salmonella I4, [5], 12∶I∶-in Maoming City from 2017 to 2019 [PDF]
, 2020 Objective To understand the epidemic characteristics and drug resistance of Salmonella I4, [5], 12∶I∶-in Maoming City. Methods Salmonella I4, [5], 12∶I∶-were identified by serological method, and then antibiotic sensitivity of isolated Salmonella I4, [5],
Guodong LIAO +3 more
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Zhongguo shiyan zhenduanxue, 2011 目的探讨临床分离的多重耐药铜绿假单胞菌耐药性及相关耐药基因。方法采用纸片扩散法进行药敏试验,VITEK 2全自动微生物鉴定及药敏系统对其进行重新鉴定,聚合酶链反应（PCR）扩增oprD2、金属酶基因、氨基糖苷类酶修饰基因。结果 40株铜绿假单胞菌对临床常用的抗生素:厄它培南,亚胺培南（IPM）、头孢他啶（CAZ）、美罗培南、头孢吡肟（PEP）、哌拉西林/他唑巴坦（TZP）、环丙沙星、氨曲南（ATM）、庆大霉素（GEN）、妥布霉素、阿米卡星、多粘菌素耐药率分别为100%、96.2%、92.0%、90.1%、
赵祝香 +4 more
doaj
Zhongguo shiyan zhenduanxue, 2021 目的研究神经重症监护病房(NICU)中肺炎克雷伯病原菌(Klebsiella pneumoniae,KP)的抗菌药物敏感性、相关耐药基因携带情况,及其与临床耐药表型的关系,为KP感染控制、预防和治疗提供科学合理的临床指导。方法收集2015年1月-2018年12月NICU病人分离出来的及病房环境中定植KP,使用K-B法检测其临床抗菌药物敏感性,同步进行超广谱β-内酰胺酶(ESBLs)表型确证试验,聚合酶链式反应检测其染色体和质粒DNA上所携带的β-内酰胺类、喹诺酮类等相关耐药基因 ...
刘妍 +5 more
doaj
Studies on Genes Related to Copper Accumulation of the Fujian Oyster Crassostrea angulata [PDF]
, 2015 牡蛎是具有重要经济价值的海水养殖品种，在世界很多地区都有养殖。而我国牡蛎养殖产量居世界首位。同时，牡蛎对铜、锌等金属具有很强的富集能力，经常被用于指示河口污染情况。随着工业发展，近岸河口区遭受越来越严重的金属污染，铜就是其中非常有代表性的金属污染物。因此牡蛎对铜的超富集现象越来越被人们所关注。学者们发现牡蛎对铜富集存在非常大的个体差异，然而相关的研究却非常有限，特别是缺少分子机制的解析。对这一现象的深入研究不但可以探寻、丰富牡蛎铜富集相关机制，更可以为选育牡蛎不同金属富集能力品系提供理论依据 ...
史博
core
Zhongguo shiyan zhenduanxue, 2013 目的了解临床分离的鲍曼不动杆菌耐药性及β-内酰胺酶耐药基因的存在类型。方法用美国BD公司Phoenix-100型全自动细菌鉴定药敏系统鉴定细菌及做药敏试验,用聚合酶链反应（PCR）方法检测5种常见β-内酰胺酶耐药基因。采用随机引物PCR扩增对菌株进行分型。结果 50株鲍曼不动杆菌对多粘菌素B全部敏感,,而对其余18种抗生素耐药率为52%-100%,其中24株为泛耐药菌株,共分为8种基因型,B型菌株为主要的流行株,所有菌株均检出TEM-1基因,46株检出AmpC基因,31株检出0XA-24基因 ...
邓丽华 +4 more
doaj
Studies on the regulation of membrane microdomains (lipid rafts) / Flot to mitogen-activated protein kinase signaling pathway and its effect on reversing multidrug resistance of hepatocellular carcinoma cells [PDF]
, 2015 目的： 1.构建耐药肝癌细胞模型。 2.检测Flot下调对ERK1/2信号通路的影响，明确能否通过Flot调控ERK1/2信号通路从而实现肝癌细胞耐药性的逆转。 3.检测脂筏破坏剂甲基-β-环糊精破坏脂筏结构对ERK1/2的影响，探索脂筏与ERK1/2之间的关联。 4.检测下调Flot对耐药肝癌细胞侵袭能力的影响。 5.构建过表达质粒Flot1/pcDNA3.1（+）、Flot2/pcDNA3.1（+），为后续研究工作奠定基础。 方法： 1.用阿霉素大剂量冲击法诱导细胞，Celltiter ...
王亚丽
core
99Tcm-N(NOEt)2 Uptake Kinetics Difference among KMB17 Human Embryonic Lung Diploid Fibroblast and Different Human Lung Cancer Cells [PDF]
, 2010 Background and objective PET/CT imaging is expensive, so searching the tumor imaging agent for SPECT/CT is necessary. 99Tcm-N(NOEt)2 [bis (N-ethoxy-N-ethyl dithiocarbamato) nitrido99Tcm (V)] can be uptaken by lung cancer cells and other cells alike.
Jianming CAO, Tiekun MA, Wei JIA
core
Etiological characteristics of two strains of human-origin Escherichia albertii in Baoshan District， Shanghai [PDF]
ObjectiveTo analyze the etiological characteristics of two strains of Escherichia albertii first identified in Baoshan District, Shanghai, in 2024, to explore their antibiotic resistance characteristics, virulence characteristics, and multilocus sequence
JIN Min +3 more
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BSD2000 Deep Hyperthermia Combined with Chemotherapy of PT regimen in Patients with Non-small Cell Lung Cancer [PDF]
, 2010 Background and objective The aim of this study is to determine the short-term efficacy, toxicity and the rate of life-quality improvement of BSD2000 deep hyperthermia combined with chemotherapy of PT regimen in patients with non-small cell lung cancer ...
Jun ZHAO, Mengxiang YANG, Yanwen WANG
core
Comparative Analysis of Genetic Differences and Probiotic Characteristics of Human Gut-Dwelling Ligilactobacillus ruminis in Kashi, Xinjiang [PDF]
This study was focused on 16 strains of Ligilactobacillus ruminis isolated from fecal samples of healthy persons from different families in Kashi, Xinjiang.
YIN Tong, AN Meiling, LIANG Zhixuan, LU Yanzhuan, SUN Hailong, NI Yongqing
core +1 more source