Results 31 to 40 of about 9,169 (174)
Veterinary Dermatology, Volume 36, Issue 3, Page 302-313, June 2025.Background – Malassezia pachydermatis (MP) is implicated in severe dermatitis and otitis externa (OE) of companion animals and recently gained attention for its increasing resistance to azole compounds. For this reason, developing novel therapeutic strategies is of great interest. In a previous work, we used reference yeast isolates to evaluate several
Costanza Spadini +11 more
wiley +1 more source
Veterinary Dermatology, Volume 36, Issue 3, Page 234-282, June 2025.Background – Canine pyoderma is one of the most common presentations in small animal practice, frequently leading to antimicrobial prescribing. Objectives – To provide clinicians with antimicrobial treatment guidelines for staphylococcal pyoderma, including those involving meticillin‐resistant staphylococci.
Anette Loeffler +9 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2006 目的建立人肝癌多药耐药细胞系并研究其耐药特性及机制。方法应用人肝癌细胞株HepG2,采用阿霉素（ADM）浓度梯度递增诱导法,建立多药耐药细胞系HepG2/ADM。相差显微镜观察细胞,用MTT法检测该耐药细胞株对多种化疗药物的多药耐药性,采用流式细胞术检测该耐药细胞表面多药耐药基因（mdr）的表达产物P糖蛋白（P-gp）、多药耐药相关蛋白（MRP）及谷胱甘肽硫转移系统（GSH/GST）的表达情况,应用RT-PCR检测MDR、MRP基因表达水平。结果HepG2/ADM对多种抗癌药物产生耐药 ...
王宁, 李玉, 刘洁
doaj
Function and mechanism of Repulsive Guidance Molecule RGMb/Dragon during Colorectal Cancer progression [PDF]
, 2016 结直肠癌是我国甚至全球范围内最常见的肿瘤之一，也是肿瘤相关死亡的主要原因之一。及时而有效的诊断是降低结直肠癌死亡率的重要措施。除了临床上常规的诊断及治疗方法外，人们也在不断致力于新型肿瘤标志物的研究，以进一步明确结直肠癌发生发展的机制。排斥导向分子(RGM)家族成员RGMb，又名Dragon，作为骨形态发生蛋白(Bonemorphogeneticprotein，BMP)通路的辅助受体，不但承载着胚胎发生、神经修复和肾脏正常生理功能，还与各种上皮来源肿瘤的发生发展密切相关。然而，RGMb ...
施颖
core
Veterinary Dermatology, Volume 36, Issue 2, Page 148-158, April 2025.Background – Biofilm production by canine otitis externa (COE) pathogens and resistance development to multiple antimicrobials are commonly reported problems in veterinary practice. The use of adjuvants to disrupt biofilms may be a viable adjunctive therapy.
Bhumika F. Savaliya +3 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2021 目的 建立人胃癌耐药细胞,为研究人胃癌耐药细胞的耐药机制以及为耐药肿瘤细胞的治疗提供耐药肿瘤细胞模型。方法 体外培养人胃癌细胞SGC-7901,用50μmol/L顺铂多次反复冲击,对冲击4次和6次的SGC-7901分别检测对顺铂的抑制率和多药耐药基因（MDR）及多药耐药特异基因片段的表达,并计算药物对细胞的半数抑制浓度（IC50）。结果 经4次和6次冲击后,受冲击的SGC-7901胃癌细胞对顺铂的抑制率明显高于亲本细胞,IC50为63.98μmol/L和66.34μmol/L,而亲本细胞SGC ...
刘玉侠 +6 more
doaj
A study about the impact of overpression of Oct4 and Sox2 gene on gastric cancer cell biology and oncology characteristics [PDF]
, 2015 目的：通过构建过表达干细胞因子Oct4和Sox2的胃癌SGC7901细胞株，研究过表达干细胞因子Oct4和Sox2对SGC7901胃癌细胞干细胞特性、肿瘤学特性、及生物学特性的影响，并探讨Oct4及Sox2在肿瘤细胞中的作用机制。 方法：1.构建携带Sox2基因的重组质粒，慢病毒载体感染SGC7901-OCT4细胞株得到SGC7901-OCT4-SOX2稳转细胞株。2.利用荧光定量PCR、western–bloting检测过表达Oct4及Sox2的胃癌SGC7901细胞中胃癌干细胞标志基因表达情况。3 ...
李文鹏
core
Combating infections under siege: Healthcare challenges amidst the military assault in Gaza
World Medical &Health Policy, Volume 17, Issue 1, Page 188-213, March 2025.Abstract The ongoing Israeli military assault on Gaza has caused a devastating collapse of healthcare infrastructure, resulting in an unprecedented surge in infectious diseases. The destruction of sanitation systems, restrictions on medical supplies, and displacement of civilians have created an environment conducive to the rapid spread of waterborne ...
Bilal Irfan +24 more
wiley +1 more source
热化疗逆转人肝癌细胞系HepG2/ADM多药耐药性的实验研究
Zhongguo shiyan zhenduanxue, 2007 目的研究热化疗对多药耐药人肝癌细胞系HepG2/ADM耐药性的影响并探讨相关的机制。方法通过MTT法测定热化疗对多药耐药人肝癌细胞系HepG2/ADM耐药性影响;利用流式细胞仪检测细胞内ADM浓度变化。结果热化疗使多药耐药人肝癌细胞系HepG2/ADM耐药性降低;细胞内阿霉素浓度提高。结论热化疗具有逆转多药耐药人肝癌细胞系HepG2/ADM耐药性的作用,可能与细胞内阿霉素浓度提高有关。
李玉, 王宁, 孙涛, 宋丽艳
doaj
A New Type of Spacer Oligonucleotide Typing for Mycobacterium Tuberculosis Complex and Clinical Evaluation [PDF]
, 2016 结核病是当今世界范围内对人类健康最具威胁的传染性疾病之一，具有潜伏感染的特点，因此追踪传染源和查证传播途径是长期困扰结核病流行病学研究的一个问题，也是分子流行病学要解决的首要问题。基因分型技术是分子流行病学研究的一个有力工具，在研究结核病传播规律方面有着不可替代的优势。一个理想的基因分型技术应当具备简便、快速、结果可在不同实验室间进行比较等特点。本论文基于探针熔解曲线分析技术，建立新型的快速结核分枝杆菌分型体系，并评价其临床应用。论文内容如下： 第一章为绪论，首先概述了结核病目前的流行情况 ...
曾小红
core