Single Nucleic Acid Molecule Manipulation and Single Cell Sequencing [PDF]
, 2017 DNA既是遗传信息的载体，也具备识别、催化以及组装等功能。DNA双螺旋结构具有固定的宽度和螺距，这种特性使得DNA成为天然的“建筑”材料。通过合理的设计可利用DNA制造复杂的二维、三维纳米结构，以及各种结构可变的DNA分子机器。但是这些纳米结构和分子机器由于缺乏实际功能，无法真正应用于生物传感、药物运输、纳米器件等关键领域，发展功能性的纳米结构以及分子机器成为DNA纳米材料领域一个重要的发展方向。 严格的碱基互补配对原则为DNA的半保留复制和遗传信息的稳定传递提供了分子基础 ...
邹远
core
Physiological Characteristics and Transcriptomic Analysis of Saccharomyces cerevisiae under Carvacrol Stress [PDF]
This study investigated the effect of carvacrol stress on the physiological characteristics and transcriptome of Saccharomyces cerevisiae, a common spoilage yeast in fruit and vegetable products.
NIU Liyuan, SUN Xiaocheng, LIU Jingfei, WU Zihao, BAI Yanhong, ZHANG Zhijian
core +1 more source
Comparative transcriptomics analysis between the fifth larvae and females of Angiostrongylus cantonensis and the expression pattern analysis of AC-14-3-3 [PDF]
, 2016 广州管圆线虫（Angiostrongyluscantonensis）是一种非常重要的人畜共患病病原寄生虫，近年来已在更广的宿主和地理分布范围内发现了该寄生虫感染的存在。然而，在公共数据库中可获得的相关资源信息非常有限，这极大地限制了人们对该寄生虫在入侵宿主、寄生生活和免疫逃避等方面机制的深入研究，也严重影响了在该病诊断、治疗和抗虫疫苗研制等领域的发展。 广州管圆线虫第五期幼虫（L5）既是可以在人和小鼠等偶然宿主脑内长期存活并引起嗜酸性脑膜脑炎的时期，又是在天然宿主体内可以突破血脑屏障进入肺动脉的时期 ...
余良
core
Transcriptomic Analysis of the Effect of the Phosphatidylinositol 3-Kinase/Protein Kinase B Signaling Pathway on Metabolic Homeostasis in Tan Sheep Muscle during Chilled Storage [PDF]
In order to uncover the role of the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) (PI3K/AKT) signaling pathway in metabolic regulation in Tan sheep meat during chilled storage, the transcriptome changes of lamb meat injected with the PI3K ...
ZHANG Qian, YANG Bo, LUO Ruiming, HU Xiaolei, BI Yongzhao, CHEN Xueyan, WANG Jinxia, LI Rong, HU Lijun
core +1 more source
Engineered CHO-K1 by Gene Edition and Its Application to Recombinant Antibody Expression [PDF]
, 2016 抗体药物是近年来发展最快的一类生物技术药物，因其极强的靶向特异性，已成功运用于多种疾病的治疗；而目前大部分抗体药物均由中国仓鼠卵巢癌（Chinesehamsterovary，CHO）细胞进行生产。CHO有多个衍生品系，其中CHO-K1在工业中运用较广；CHO-K1主 要有两个品系CHO-K1-CCL-61和CHO-K1-CRL-9618，初始均以血清依赖的贴壁培养方式进行培养，工业生产中通常需要对其进行无血清悬浮适应，甚至对其基因组进行适当的改造；目前CHO-K1-CCL-61的运用和研究较为广泛 ...
游敏
core
Transcriptional regulatory network analysis of microglia in multiple sclerosis [PDF]
Objective·To investigate the differential gene expression of microglia in the gray and white matter of multiple sclerosis (MS) using single-nucleus transcriptomic analysis, aiming to explore their roles in disease progression, and identify key ...
CAI Qiangwei +5 more
core +1 more source
Population genetic variation and symbiotic and associated bacteria diversity studies of bryozoan Bugula neritina based on high-throughput sequencing [PDF]
, 2016 多室草苔虫是一种重要的药源生物，从这种生物体内提取出的多种草苔虫内酯被证明有着较强的抗癌活性，其中草苔虫内酯bryostatin1是治疗阿尔茨海默症和多种癌症的最有希望的药物来源，而草苔虫内酯bryostatin1的产生与多室草苔虫体内的共生细菌“CandidatusEndobugulasertula”密切相关。然而，国内学者从中国沿海的多室草苔虫中却一直未能成功提取出这种最具药用价值的草苔虫内酯bryostatin1 ...
李海
core
Zhongshan Daxue xuebao. Yixue kexue ban, 2010 【目的】 探讨鼻咽癌细胞中启动EB病毒核抗原1(EBNA1蛋白)表达的EB病毒Q启动子(Qp)的变异特征,比较有第62 225位点(g→a)和第62 422位点(g→c)两个点突变的Qp与原型Qp(B95.8型)的功能学差异及其生物学意义65377;【方法】 采用PCR的方法扩增29例鼻咽癌组织石蜡标本和14例健康成人外周血标本(总共43例)中的Qp序列,PCR产物测序后分析其突变情况,统计学分析Qp中的点突变与鼻咽癌的相关性65377;把突变型和原型Qp分别克隆到荧光素酶报告基因载体上,检测相对光强度(
doaj
实时荧光定量RT-PCR检测siRNA表达载体抑制人肾癌786-0细胞G250 mRNA的表达
Zhongguo shiyan zhenduanxue, 2011 目的评价实时荧光定量逆转录聚合酶链反应（RT-PCR）法检测小干扰RNA（siRNA）表达载体抑制人肾癌786-0细胞G250 mRNA表达的可靠性,以建立稳定的RNAi技术。方法体外合成四条针对G250基因的siRNA,并设计阴性对照和空白对照,转染肾癌786-0细胞株;根据G250 mRNA的序列设计特异性引物,荧光染料SYBR Green Ⅰ法定量RT-PCR测定G250 mRNA的表达情况。结果四条siRNA分别使G250 mRNA表达量降低了35.56%、49.27%、45.88%、65.13%
赵俊峰 +5 more
doaj
Establishment of a prognostic risk scoring model for patients with pancreatic ductal adenocarcinoma based on cell ferroptosis-related genes and its application value [PDF]
Objective To establish a prognostic risk scoring model for patients with pancreatic ductal adenocarcinoma (PDAC) based on cell ferroptosis-related genes, and to investigate its application value.
QING Gong, JING Xue, JIANG Yueping
core +1 more source