TCTN1 promotes epithelial⁃mesenchymal transition to mediate trastuzumab resistance in HER2⁃positive breast cancer [PDF]
"Objective To explore the key genes mediating trastuzumab resistance in human epidermal growth factor receptor 2⁃positive（HER2+）breast cancer and their molecular mechanisms.
CHEN Luhong*，GAO Hao，JI Jie，ZHU Xiaochao
core +2 more sources
重组pDC316-MCMV／hNIS真核表达质粒的构建及在肿瘤细胞的功能
Zhongshan Daxue xuebao. Yixue kexue ban, 2007 【目的】克隆人的钠／碘同向转运体（hNIS）基因可编码序列，并研究其在非甲状腺肿瘤细胞内的功能表达。【方法】利用逆转录和聚合酶链反应（RT-PCR）从毒性弥漫性甲状腺肿（又称Graves病）病人的甲状腺组织中扩增得到NIS的可编码区基因，并克隆到T载体，测序后亚克隆到腺病毒载体穿梭质粒pDC316载体上，获得重组真核表达质粒载体pDC316-MCMV／hNIS。采用脂质体转染的方法，把pDC316-MCMV／hNIS重组质粒（实验组）或pDC316空质粒（对照组）分别导人到胰腺癌细胞株CAPAN-Ⅱ细胞、
周泉波 +5 more
doaj
Effects of 1-MCP on the genes related to wax anabolism in pear peel based on transcriptomics [PDF]
[Objective] According to the investigation and laboratory research of our study group for several years, it has been found that the main pear cultivar Yuluxiang was easy to become greasy in the early stage of shelf life at room temperature, and also in ...
JIA Xiaohui +4 more
core +1 more source
Zhongguo shiyan zhenduanxue, 2007 目的探讨PAI的发卡样siRNA真核表达载体PAI siRNA对人肝癌HpG2细胞VEGF表达的影响。方法设计短发夹状PAI siRNA的寡核苷酸链,将其插入到真核细胞表达载体pSilencerTM 2.1-U6 neo载体中,构建的重组载体后;采用脂质体转染方法将构建的重组载体导入人肝癌细胞HpG2中,采用逆转录聚合酶链反应（RT-PCR）、westernblot法分别检测转染细胞PAI mRNA和蛋白的表达变化以及对VEGF表达的影响。结果经测序证明PAI siRNA序列正确;转染PAI ...
杜建时, 赵晴, 张静菊, 王嘉桔
doaj
The observation on the Oncomelania hupensis in ecological environment, the culturation of young snails and its cloning, expression, antibody preparation of thioredoxin and immunofluorescence localization [PDF]
, 2016 第一部分：鄱阳湖星子县区日本血吸虫病媒介钉螺生存情况调查和钉螺感染外睾吸虫情况及阴性钉螺的培育 近年来，本实验室经过2000-2014年的长期研究，发现先感染外睾吸虫的湖北钉螺再感染日本血吸虫，会出现日本血吸虫幼虫被杀死的现象。并且感染外睾吸虫后再感染日本血吸虫的间隔时间越长，幼虫的死亡速度越快，但其具体的控制机理尚未完全弄清楚，因此我们致力于了解外睾吸虫幼虫在自然界感染钉螺情况的调查，并探索其深层次的调节机制。在宏观方面，本部分主要是在江西鄱阳湖采集湖北钉螺进行野外调查 ...
彭午弦
core
Linchuang shenzangbing zazhi, 2014 目的 构建重组人Orail基因真核表达载体,观察其在小鼠肾小球足细胞株（MPC5）中的表达。方法 根据GenBank数据库检索到的人Orail基因序列,人工合成法合成Orail基因编码区（coding sequence,CDS）全长序列,克隆至质粒表达载体PG-CMV-IRES-EGFP,构建重组质粒载体PG-CMV-Orail,通过PCR、双酶切及基因测序等方法进行鉴定;以脂质体转染法将重组质粒转染至MPC5细胞,应用荧光显微镜观察绿色荧光检测转染效率,RT-PCR、Western ...
魏冬月
doaj
Mechanism of transforming growth factor-β1 induce renal fibrosis based on transcriptome sequencing analysis. [PDF]
Zhejiang Da Xue Xue Bao Yi Xue Ban, 2023 Li H +6 more
europepmc +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2000 【目的】构建携 HBx 基因的逆转录病毒载体, 为研究 HBx 基因与肝癌生物学行为间的关系提供基础。【方法】采 用 PCR 技术从 HBV 全基因组中扩增 HBx 基因; 将 HBx 基因亚克隆至质粒 pLNSX, 构建质粒 pLNSHBx,磷酸钙-DNA 共沉淀 法将重组体导入包装细胞系 PA317, 检测培养上清病毒滴度。【结果】应用 PCR 技术成功地从 HBV 基因组中克隆出全长 HBx 基因, 并经序列分析证实; 建立了产病毒细胞株 PA317/HBx,检测其培养上清病毒滴度为 8.
闵 军, 刘彦文, 陈积圣
doaj
TIPE regulates glucometabolic reprogramming by modulating LDHA expression in triple-negative breast cancer [PDF]
Background and purpose: Tumor necrosis factor alpha-induced protein 8 (TNFAIP8), also called TIPE, plays critical regulatory roles in various malignancies, yet its molecular mechanisms in metabolic reprogramming of triple-negative breast cancer (TNBC ...
HU Wei, REN Xiaomeng, WANG Yang, ZHAO Peiqing, CAO Kai
core +1 more source
[Transcriptome sequencing for identification of the genes associated with restenosis of venous grafts in rabbits]. [PDF]
Nan Fang Yi Ke Da Xue Xue Bao, 2023 Zhou H, Yang L, Xie S, Yang J, Zhang J.
europepmc +1 more source