Transcriptome Analysis of Cryptolestes ferrugineus under Low-temperature Stress [PDF]
Cryptolestes ferrugineus, a cosmopolitan stored-grain pest, was studied using transcriptome sequencing to investigate its molecular response to low-temperature stress..
FANG Zhi-yi +5 more
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Physiological and molecular basis of cold and disease resistance heterosis in Haliotis diversicolor hybrid [PDF]
, 2017 “东优1号”杂色鲍（Haliotis.diversicolor），是以杂色鲍台湾群体为母本，杂色鲍日本群体为父本的杂交鲍新品种，相比亲本具有高水平的抗性杂种优势，表现出高于亲本的越冬养殖存活率。目前，该新品种已在生产上得到广泛的推广和应用，在相当程度上解决了杂色鲍冬季暴发性死亡的问题。然而，对其高存活率杂种优势的生理基础和分子机制，尚缺乏较为深入的了解。为了在鲍的育种生产中更科学、有效地利用杂种优势理论，也为了更清晰地认识贝类杂种优势的分子机制，本文结合生理学实验和分子生物学手段 ...
梁爽
core
Integrated single-cell and transcriptome sequencing to construct a prognostic model of M2 macrophage-related genes in prostate cancer [PDF]
ObjectiveTo explore the prognostic value of M2 macrophage-related genes in prostate cancer (PCa), aiming to predict tumor prognosis more accurately and enable personalized treatment.Methods·RNA sequencing (RNA-seq) data of PCa were downloaded from The ...
FENG Chengling, HAN Bangmin, TANG Kairan
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In vitro therapeutic effects and molecular mechanisms of targeted inhibition of CDK12/13 in high-grade gliomas [PDF]
, 2023 Objective·To find novel and common targeting strategies for high-grade gliomas (HGGs) from the perspective of epigenetic and transcriptional modulators, test the therapeutic effect in vitro and investigate the related molecular mechanisms.Methods ...
HAN Yujie +4 more
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野生动物学报, 2018 旨在研究不同生长时期东北梅花鹿茸生长中心软骨组织基因表达变化,为鹿茸快速生长机制研究提供理论依据。采用Illumina测序技术和生物信息学方法对快速生长期和骨化期鹿茸生长中心软骨组织进行转录组测序、组装、比对和差异基因表达分析。从快速生长期和骨化期鹿茸软骨组织中分别获得4.47和4.45 Gb的测序数据,测序质量值Q20值分别为98.44和98.05。通过Trinity软件组装,分别获得39377和37331条组装序列,平均长度分别为1138和1143 nt ...
刘美欣 赵 雨 张 梅 刘宇昕 胡耀中 幺宝金
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Development, optimization and preliminary application of in vitro models supporting HBV infection [PDF]
, 2016 乙型肝炎病毒（HepatitisBvirus，HBV）感染是危害人类健康的重要公共卫生问题，尽管现有的预防性疫苗已显著减少了新发HBV感染，全球仍有约2.4亿的慢性HBV感染者需要得到有效的治疗。鉴于现有慢乙肝治疗药物只能控制病情发展而难以实现临床治愈，发展更加有效的Anti-HBV新药是当前HBV的研究重点方向。建立和发展能实现HBV完整生命周期的细胞和动物模型对Anti-HBV治疗新技术的发展至关重要。其中，支持HBV感染和复制的细胞模型是研究HBV与宿主相互作用、筛选和评估HBV感染治疗药物 ...
赵菁华
core
Screening of Anti-hyperuricemia Peptides from Oat Protein Based on High-throughput Transcriptome Sequencing and Network Pharmacology [PDF]
Initiating from omics, the research aimed to discover and filter oat active peptides effective against hyperuricemia, and to study the operational mechanism of these oat active peptides in treating hyperuricemia.
Fei GAO +5 more
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Transcriptomic analysis of the root system of Platycodon grandiflorum in response to the fallen leaves of Acer truncatum [PDF]
[Objective] The aim of this study was to explore the effect of Acer truncatum fallen leaves in Beijing on the accumulation of medicinal active ingredients of Platycodon grandiflorum and to reveal the molecular mechanism of active ingredient synthesis ...
CHENG Shaoying +5 more
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Zhongshan Daxue xuebao. Yixue kexue ban目的应用纳米孔长读长测序技术，深入分析正常肝实质细胞和肝癌细胞在低氧环境下的特异性转录组变化，以期阐明肿瘤细胞在低氧条件下生存与转移的内在机制，从而为肿瘤的精准靶向治疗开辟新的视角与策略。方法实验选取正常肝实质细胞系THLE-3与肝癌细胞系Hep3B作为研究对象，分别置于常氧与低氧条件下培养48 h后，提取其总RNA。随后，采用纳米孔测序技术，对这两种细胞系在不同氧浓度下的转录组进行高通量、高精度的测序分析。结果本研究成功构建了基于纳米孔三代测序技术的低氧转录本数据集，展现了前所未有的测序精度。通过GO（
高力, 沈晟齐, 高平
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野生动物学报, 2018 随着近年来高通量测序技术的发展,其在生物学研究领域的应用也越来越广泛,并逐渐成为生物学研究的重要方法和工具。基于高通量测序的 RNA-seq技术为揭示野生动物转录组信息提供了更为准确、便捷的方法。本文论述了RNA-seq技术的发展历史和技术特点,并结合野生动物转录组研究中所面临的问题探讨了RNA-seq技术的具体应用和发展方向,以期为野生动物的研究提供方法指导。
肖 慧 吴 尽 赵敏蝶 丁 新 刘学东
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