Zhongguo shiyan zhenduanxue, 2022 ml或5 g,HRV定性检测采用酶联免疫吸附(ELISA)法,试剂为世界卫生组织(WHO)提供;G型、P型分型采用逆转录-聚合酶链反应(RT-PCR),引物序列及试验方法均由国家疾病预防控制中心(CDC)提供;HuCV、EAdV和HAstV用RT-PCR、PCR方法检测病毒核酸,引物序列及试验方法亦由国家CDC提供。1.3统计分析应用Excel2020统计软件进行数据整理及分析,计数资料用占比或率表示;应用SPSS 25.0进行卡方检验 ...
doaj
Key enzymes and their roles in the formaldehyde-degrading process in Pseudomonas putida IOFA1 [PDF]
, 2014 Pseudomonasputida具有复杂的降解有机物代谢系统，因此经常做为模式菌应用于污水和废物的降解研究中。迄今为止，Pseudomonasputida已被发现可降解很多有毒化合物，但相关降解代谢机理的研究较少。我们以从深海沉积物中筛选到的高效甲醛降解菌Pseudomonasputida.IOFA1为对象，选取该菌株降解甲醛过程中的3个时间点，即A组（未加入甲醛），B组（加入甲醛20min）和C组（甲醛降解后2h）进行转录组测序，通过转录组变化的比较来探索该菌的甲醛代谢机制 ...
余翔
core
Zhongshan Daxue xuebao. Yixue kexue ban, 2001 【目的】构建人Ⅰ 型基质金属蛋白酶基因(MMP1)真核表达重组质粒, 并进行序列分析。【方法】用逆转录聚 合酶链反应扩增人Ⅰ 型基质金属胶原酶cDNA, 获得目的基因片段(1 407 bp)连接至pcDNA3 载体, 并转化大肠杆菌DH5α, 筛 选阳性克隆并鉴定, 并对片断全长进行DNA 序列测定。【结果】所克隆人Ⅰ 型基质金属胶原酶cDNA, 含全长MMP1 编码区, 与GeneBank 公布序列比较, 仅1 318 位的胞苷酸C 突变为腺苷酸A。并成功构建了含有MMP1 ...
彭 慧, 汪 谦, 黄洁夫
doaj
[Functional characteristics of YAP-positive hepatocytes expression in an early stage of NASH with transcriptome sequence analysis]. [PDF]
Zhonghua Gan Zang Bing Za Zhi, 2022 Zeng WL, Liang JE, Liu YX, Wang Y.
europepmc +1 more source
Study on HMW-PAHs metabolic pathway and transcriptional regulation in Novosphingobium pentaromativorans US6-1 [PDF]
, 2014 多环芳烃（PAHs）是一类在环境中广泛分布的有机化学污染物，具有“三致”（致癌、致畸、致突变）效应，通过食物链的传递会对生态环境和人体健康造成极大危害。目前研究结果表明，微生物的降解与转化是消除PAHs污染的有效方式之一。以苯并芘（BaP）为代表的高分子量PAHs（HMW-PAHs）因其结构稳定、难降解，可在环境中长期存在。能以BaP为唯一碳源和能源进行矿化的微生物研究报道较少，代谢路径也不够清楚。本论文以一株筛选自海洋环境的 ...
杨璐溪
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Establishment of a prognostic risk scoring model for patients with pancreatic ductal adenocarcinoma based on cell ferroptosis-related genes and its application value [PDF]
Objective To establish a prognostic risk scoring model for patients with pancreatic ductal adenocarcinoma (PDAC) based on cell ferroptosis-related genes, and to investigate its application value.
QING Gong, JING Xue, JIANG Yueping
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Zhongguo shiyan zhenduanxue, 2004 目的 构建人VEGF16 5腺病毒表达载体 ,探讨应用血管内皮生长因子 （VEGF）基因治疗脑梗死的可行性。方法 采用分子克隆技术 ,以质粒hVEGF16 5·SR为模板 ,扩增hVEGF16 5成熟cDNA。经穿梭质粒PShuttle克隆到腺病毒质粒上 ,构成Adeno VEGF16 5腺病毒重组质粒 ,经酶切及测序鉴定正确后 ,包装成为重组Adeno VEGF16 5腺病毒 ,并进行电镜观察及滴度测定。用免疫印迹及免疫组化方法检测VEGF蛋白的表达。结果 ...
张海鸥, 吴军, 林凯华, 易黎
doaj
Physiological response of sunflowers to broomrape parasitism and analysis of the key metabolic pathways [PDF]
[Objective] To study the physiological response of sunflowers to broomrape parasitism and the related metabolic pathways, as well as understanding the molecular mechanism of sunflower resistance to broomrape parasitism. [Methods] The broomrape-resisant
BAO Haizhu +5 more
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Engineered CHO-K1 by Gene Edition and Its Application to Recombinant Antibody Expression [PDF]
, 2016 抗体药物是近年来发展最快的一类生物技术药物，因其极强的靶向特异性，已成功运用于多种疾病的治疗；而目前大部分抗体药物均由中国仓鼠卵巢癌（Chinesehamsterovary，CHO）细胞进行生产。CHO有多个衍生品系，其中CHO-K1在工业中运用较广；CHO-K1主 要有两个品系CHO-K1-CCL-61和CHO-K1-CRL-9618，初始均以血清依赖的贴壁培养方式进行培养，工业生产中通常需要对其进行无血清悬浮适应，甚至对其基因组进行适当的改造；目前CHO-K1-CCL-61的运用和研究较为广泛 ...
游敏
core
Transcriptomic Analysis of the Effect of the Phosphatidylinositol 3-Kinase/Protein Kinase B Signaling Pathway on Metabolic Homeostasis in Tan Sheep Muscle during Chilled Storage [PDF]
In order to uncover the role of the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) (PI3K/AKT) signaling pathway in metabolic regulation in Tan sheep meat during chilled storage, the transcriptome changes of lamb meat injected with the PI3K ...
ZHANG Qian, YANG Bo, LUO Ruiming, HU Xiaolei, BI Yongzhao, CHEN Xueyan, WANG Jinxia, LI Rong, HU Lijun
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