Results 101 to 110 of about 505,000 (387)

16S rRNA gene sequencing of mock microbial populations- impact of DNA extraction method, primer choice and sequencing platform [PDF]

, 2016
peer-reviewedBackground Next-generation sequencing platforms have revolutionised our ability to investigate the microbiota composition of complex environments, frequently through 16S rRNA gene sequencing of the bacterial component of the
Claesson, Marcus J., Clooney, Adam G, Cotter, Paul D., Fouhy, Fiona, STANTON, CATHERINE   +4 more
core   +2 more sources

Absolute quantitation of microbes using 16S rRNA gene metabarcoding: A rapid normalization of relative abundances by quantitative PCR targeting a 16S rRNA gene spike‐in standard

MicrobiologyOpen, 2020
Metabarcoding of the 16S rRNA gene is commonly used to characterize microbial communities, by estimating the relative abundance of microbes. Here, we present a method to retrieve the concentrations of the 16S rRNA gene per gram of any environmental ...
O. Zemb, C. Achard, J. Hamelin, M. De Almeida, B. Gabinaud, L. Cauquil, L. Verschuren, J. Godon   +7 more
semanticscholar   +1 more source

Establishment of a coculture system for Porphyromonas gingivalis and head and neck squamous cell carcinoma using spheroid culture and LATS inhibition

FEBS Open Bio, EarlyView.
We established a spheroid coculture system enabling viable Porphyromonas gingivalis–HNSCC interactions under normoxic conditions. Inhibition of LATS1/2 maintains tumor cells in an undifferentiated state, which may promote spheroid growth and create a more permissive environment for bacterial persistence.
Yurika Nakajima, Shogo Okazaki, Muneaki Tamura, Shuichi Sato, Kenichi Imai   +4 more
wiley   +1 more source

rRNA operon improves species-level classification of bacteria and microbial community analysis compared to 16S rRNA

Microbiology Spectrum
Precise identification of species is fundamental in microbial genomics and is crucial for understanding the microbial communities. While the 16S rRNA gene, particularly its V3-V4 regions, has been extensively employed for microbial identification ...
Sohyoung Won, Seoae Cho, Heebal Kim
doaj   +1 more source

Comparison of P1 and 16S rRNA genes for detection of Mycoplasma pneumoniae by nested PCR in adults in Zhejiang, China

Journal of Infection in Developing Countries, 2015
Introduction: Mycoplasma pneumoniae (M. pneumoniae) is the most common atypical pathogen that causes respiratory infections in humans. Laboratory tests are important in the diagnosis of M. pneumoniae because of the atypical features in clinical signs and
Zibo Zhou, Xiangzhi Li, Xiaojian Chen, Lili Yao, Changwang Pan, Huicong Huang, Fangjun Luo, Xiaoping Zheng, Xiaojing Sun, Feng Tan   +9 more
doaj   +1 more source

Evaluation of 16S rRNA gene primer pairs for bacterial community profiling in an across soil and ryegrass plant study [PDF]

, 2023
Shengjing Shi, Sandeep Kumar, Sandra Young, Paul D. MacLean, Ruy Jáuregui   +4 more
openalex   +1 more source

Design and evaluation of 16S rRNA sequence based oligonucleotide probes for the detection and quantification of Comamonas testosteroni in mixed microbial communities [PDF]

, 2006
Background The β-proteobacterial species Comamonas testosteroni is capable of biotransformation and also biodegradation of a range of chemical compounds and thus potentially useful in chemical manufacturing and bioremediation.
Bathe, Stephan, Hausner, Martina
core   +2 more sources

Domain associated with zinc fingers‐containing NF90‐NF45 complex inhibits m6A modification of primary microRNA by suppressing METTL3/14 activity

FEBS Open Bio, EarlyView.
NF90–NF45 functions as a negative regulator of methyltransferase‐like 3/14 (METTL3/14)‐mediated N6‐methyladenosine (m6A) modification on primary microRNAs (pri‐miRNAs). NF90–NF45 binds to anti‐oncogenic pri‐miRNAs and inhibits their m6A modification, thereby suppressing the biogenesis of anti‐oncogenic miRNAs.
Takuma Higuchi, Shunsuke Morioka, Keiko Morisawa, Kazutsugu Matsukawa, Shingo Ashida, Takeshi Suzuki, Shuji Sakamoto   +6 more
wiley   +1 more source

Marinomonas brasilensis sp. nov., isolated from the coral Mussismilia hispida, and reclassification of Marinomonas basaltis as a later heterotypic synonym of Marinomonas communis [PDF]

, 2011
A Gram-negative, aerobic bacterium, designated strain R-40503(T), was isolated from mucus of the reef-builder coral Mussismilia hispida, located in the Sao Sebastiao Channel, Sao Paulo, Brazil.
Alves, Anne Willems, Baumann, Eck, Fabiano L. Thompson, Goris, Ilse Cleenwerck, Luciane A. Chimetto, Marcelo Brocchi, Paul De Vos, Saitou, Savelkoul, Stackebrandt, van Landschoot, Willems   +14 more
core   +2 more sources

Full-length 16S rRNA gene sequencing by PacBio improves taxonomic resolution in human microbiome samples

BMC Genomics
Background Sequencing variable regions of the 16S rRNA gene (≃300 bp) with Illumina technology is commonly used to study the composition of human microbiota.
E. Buetas, M. Jordán-López, A. López-Roldán, Giuseppe D’Auria, L. Martínez-Priego, Griselda de Marco, M. Carda-Diéguez, Álex Mira   +7 more
semanticscholar   +1 more source