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Acquisition of 16S rRNA methylase gene in Pseudomonas aeruginosa

The Lancet, 2003
Bacteria develop resistance to aminoglycosides by producing aminoglycoside-modifying enzymes such as acetyltransferase, phosphorylase, and adenyltransferase. These enzymes, however, cannot confer consistent resistance to various aminoglycosides because of their substrate specificity.
Keiko, Yokoyama   +8 more
openaire   +2 more sources

Diversity of 16S rRNA gene, ITS region and aclB gene of the Aquificales

Extremophiles, 2006
The Aquificales are prevalent members of the microbial communities inhabiting many marine and terrestrial hydrothermal systems. Numerous new strains were obtained from deep-sea and terrestrial hydrothermal systems. In order to resolve the phylogenetic relationships within this group, three different phylogenetic datasets were used, namely the 16S rRNA ...
I, Ferrera   +5 more
openaire   +2 more sources

Transfer RNA genes between 16S and 23S rRNA genes in rRNA transcription units of E. Coli

Cell, 1976
We have identified genes for tRNAGLU/2 on the transducing phages o80d3ilvsu7+ (see Ohtsubo et al., 1974) and lambdarifd18 (Kirschbaum and Konrad, 1973), and a gene for tRNAlle/1 on the transducing phage o80rifr (Konrad, Kirschbaum, and Austin, 1973). All these phages have previously been shown to carry genes for rRNA (Ohtsubo et al., 1974; Lindahl et ...
E, Lund   +5 more
openaire   +2 more sources

16S rRNA Gene Probes for Deinococcus species

Systematic and Applied Microbiology, 1996
Summary Oligonucleotide probes specific for members of the unusually radiation-resistant genus Deinococcus were designed and tested. Putative probes were identified by selecting 16S rRNA gene sequences unique to Deinococcus radiodurans UWO 298 (ATCC 35073).
Mark G. Wise   +2 more
openaire   +1 more source

Nested PCR and RFLP Analysis Based on the 16S rRNA Gene

2012
Current phytoplasma detection and identification methods are primarily based on nested polymerase chain reaction followed by restriction fragment length polymorphism analysis and gel electrophoresis. These methods can potentially detect and differentiate all phytoplasmas including those previously not described.
DUDUK, BOJAN   +3 more
openaire   +4 more sources

Organization of the ribosomal RNA genes in Mycoplasma hyopneumoniae: The 5S rRNA gene is separated from the 16S and 23S rRNA genes

Molecular and General Genetics MGG, 1986
In order to study the organization of the ribosomal RNA genes of Mycoplasma hyopneumoniae the rRNA genes were cloned in phage vectors lambda EMBL3 and lambda EMBL4. By subcloning the restriction fragments into various plasmids and analysing the resulting clones by Southern and Northern blot hybridization, a restriction map of the rRNA genes was ...
C, Taschke   +3 more
openaire   +2 more sources

Targeted 16S rRNA Gene Sequencing for Water Samples

The choice of variable region to amplify in 16S rRNA-targeted amplicon sequencing has long been a matter of debate. Here, we describe a method for sequencing multiple variable regions with the Ion 16S Metagenomics kit, which amplifies six different amplicons covering seven 16S variable regions.
Mélanie Pimenta   +2 more
openaire   +2 more sources

Detection of Methylobacterium species by 16S rRNA gene-targeted PCR

Applied and Environmental Microbiology, 1997
We designed PCR primers for specific amplification of the 16S rRNA genes of seven species of the genus Methylobacterium. All of the pairwise species tested were successfully differentiated by PCR detection with a combination of five primer sets, with the exception of M. extorquens and M. rhodesianum.
T, Nishio, T, Yoshikura, H, Itoh
openaire   +2 more sources

[PCR for the detection of 16S rRNA gene bacteria].

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University, 2002
According to the high conservative region of 16S rRNA gene in bacteria, PCR primers of the broad-range bacteria, gram-positive bacteria and gram-negative bacteria were synthesized to detect 13 bacterium species and (40) clinical specimens. All the tested bacterium species were positive.
C, Li   +6 more
openaire   +1 more source

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