Characterization of the norovirus 3C-like protease
Virus Research, 2005 The recombinant 3C-like protease of Chiba virus, a Norovirus, expressed in Escherichia coli cells was purified and characterized as to effects of pH, temperature, salt contents, and SH reagents on its proteolytic activity. The optimal pH and temperature of the 3C-like protease for the proteolytic activity were 8.6 and 37 degrees C, respectively ...
Someya, Yuichi +2 more
openaire +2 more sources
Distinct Mechanisms of Pathogenic DJ-1 Mutations in Mitochondrial Quality Control [PDF]
, 2018 The deglycase and chaperone protein DJ-1 is pivotal for cellular oxidative stress responses and mitochondrial quality control. Mutations in PARK7, encoding DJ-1, are associated with early-onset familial Parkinson’s disease and lead to pathological ...
Abeti +66 more
core +3 more sources
Sequence analysis and genomic organization of Aphid lethal paralysis virus: a new member of the family Dicistroviridae [PDF]
, 2002 The complete nucleotide sequence of the genomic RNA of an aphid-infecting virus, Aphid lethal paralysis virus (ALPV), has been determined. The genome is 9812 nt in length and contains two long open reading frames (ORFs), which are separated by an ...
Clerivet, A. +5 more
core +2 more sources
Design, synthesis, and bioevaluation of viral 3C and 3C-like protease inhibitors
Bioorganic & Medicinal Chemistry Letters, 2013 A class of tripeptidyl transition state inhibitors containing a P1 glutamine surrogate, a P2 leucine, and a P3 arylalanines, was found to potently inhibit Norwalk virus replication in enzyme and cell based assays. An array of warheads, including aldehyde, α-ketoamide, bisulfite adduct, and α-hydroxyphosphonate transition state mimic, was also ...
Prior, Allan M. +8 more
openaire +3 more sources
Switching from a protease inhibitor-based regimen to a dolutegravir-based regimen : a randomized clinical trial to determine the effect on peripheral blood and ileum biopsies from antiretroviral therapy-suppressed human immunodeficiency virus-infected individuals [PDF]
, 2018 Background: Optimization of combination antiretroviral therapy (cART) can impact the human immunodeficiency virus (HIV) reservoir. We evaluated the effect on the HIV reservoir in peripheral blood and ileum biopsies in patients switching from boosted ...
Blanco, Julià +15 more
core +3 more sources
PLoS ONE, 2013 Human Rhinovirus (HRV) infection results in shut down of essential cellular processes, in part through disruption of nucleocytoplasmic transport by cleavage of the nucleoporin proteins (Nups) that make up the host cell nuclear pore.
Erin J Walker +7 more
doaj +1 more source
Enterovirus 71 3C protease cleaves a novel target CstF-64 and inhibits cellular polyadenylation. [PDF]
PLoS Pathogens, 2009 Identification of novel cellular proteins as substrates to viral proteases would provide a new insight into the mechanism of cell-virus interplay. Eight nuclear proteins as potential targets for enterovirus 71 (EV71) 3C protease (3C(pro)) cleavages were ...
Kuo-Feng Weng +3 more
doaj +1 more source
The expression and purification of human rhinovirus protease 3C [PDF]
European Journal of Biochemistry, 1989 Human rhinovirus type 14 protease 3C was expressed as a soluble and active protein in Escherichia coli. The protease was purified by a cationic‐exchange step followed by gel filtration on a TSK 3000 column. The final yield of purified protease was in the range 0.5–1.0 mg/l culture grown to A550= 1.0.
J A, Knott +4 more
openaire +2 more sources
Generation of virus-specific cytotoxic T cells in vitro II. Induction requirements with functionally inactivated virus preparations [PDF]
, 1980 Using noninfectious Sendai virus preparations after selective enzymatic digestion of either of the two viral envelope glycoproteins, it was possible to study the effect of different virion-cell membrane interactions on virus-specific cytotoxic T ...
Bosslet +27 more
core +1 more source
Regulation of Poliovirus 3C Protease by the 2C Polypeptide [PDF]
Journal of Virology, 2004 ABSTRACT Poliovirus-encoded nonstructural polypeptide 2C is a multifunctional protein that plays an important role in viral RNA replication. 2C interacts with both intracellular membranes and virus-specific RNAs and has ATPase and GTPase activities.
Rajeev, Banerjee +4 more
openaire +2 more sources