FEBS Letters, EarlyView.This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva +5 more
wiley +1 more source
Breast Cancer Stem Cell Culture and Enrichment Using Poly(ε-Caprolactone) Scaffolds
Molecules, 2016 The cancer stem cell (CSC) population displays self-renewal capabilities, resistance to conventional therapies, and a tendency to post-treatment recurrence. Increasing knowledge about CSCs’ phenotype and functions is needed to investigate new therapeutic
Sònia Palomeras +6 more
doaj +1 more source
Glutathione-triggered formation of a Fmoc-protected short peptide-based supramolecular hydrogel. [PDF]
PLoS ONE, 2014 A biocompatible method of glutathione (GSH) catalyzed disulfide bond reduction was used to form Fmoc-short peptide-based supramolecular hydrogels. The hydrogels could form in both buffer solution and cell culture medium containing 10% of Fetal Bovine ...
Yang Shi +5 more
doaj +1 more source
Design and fabrication of 3D-printed anatomically shaped lumbar cage for intervertebra disc (IVD) degeneration treatment [PDF]
, 2016 Spinal fusion is the gold standard surgical procedure for degenerative spinal conditions when conservative therapies have been unsuccessful in rehabilitation of patients.
Ahmed M +11 more
core +2 more sources
Modeling human trophoblast, the placental epithelium at the maternal fetal interface. [PDF]
, 2020 Appropriate human trophoblast lineage specification and differentiation is crucial for the establishment of normal placentation and maintenance of pregnancy. However, due to the lack of proper modeling systems, the molecular mechanisms of these processes
Bui, Tony +3 more
core +1 more source
In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS
FEBS Letters, EarlyView.We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka +11 more
wiley +1 more source
Human adipose-derived stem cells support the growth of limbal stem/progenitor cells. [PDF]
PLoS ONE, 2017 The most efficient method to expand limbal stem cells (LSCs) in vitro for clinical transplantation is to culture single LSCs directly on growth-arrested mouse fibroblast 3T3 cells.
Hua Mei +5 more
doaj +1 more source
A Preliminary Study on Using Multi-Nozzle Polymer Deposition System to Fabricate Composite Alginate/Carbon Nanotube Tissue Scaffolds [PDF]
, 2006 Three-dimensional composite alginate/single wall carbon nanotube (SWCNT) scaffolds encapsulated with endothelial cells were fabricated by a multi-nozzle biopolymer freeform deposition system.
Guceri, S. +3 more
core +1 more source
FEBS Letters, EarlyView.Bone metastasis in prostate cancer (PCa) patients is a clinical hurdle due to the poor understanding of the supportive bone microenvironment. Here, we identify stearoyl‐CoA desaturase (SCD) as a tumor‐promoting enzyme and potential therapeutic target in bone metastatic PCa.
Alexis Wilson +7 more
wiley +1 more source
3D cell culture models: Drug pharmacokinetics, safety assessment, and regulatory consideration
Clinical and Translational Science, 2021 Nonclinical testing has served as a foundation for evaluating potential risks and effectiveness of investigational new drugs in humans. However, the current two‐dimensional (2D) in vitro cell culture systems cannot accurately depict and simulate the rich
Hongbing Wang +20 more
doaj +1 more source