Results 61 to 70 of about 388,478 (262)

Pericentromeric heterochromatin is hierarchically organized and spatially contacts H3K9me2 islands in euchromatin.

PLoS Genetics, 2020
Membraneless pericentromeric heterochromatin (PCH) domains play vital roles in chromosome dynamics and genome stability. However, our current understanding of 3D genome organization does not include PCH domains because of technical challenges associated ...
Yuh Chwen G Lee, Yuki Ogiyama, Nuno M C Martins, Brian J Beliveau, David Acevedo, C-Ting Wu, Giacomo Cavalli, Gary H Karpen   +7 more
doaj   +1 more source

Disruption of the 3D Cancer Genome Blueprint [PDF]

Epigenomics, 2016
Recent advances in chromosome conformation capture technologies are improving the current appreciation of how 3D genome architecture affects its function in different cell types and disease. Long-range chromatin interactions are organized into topologically associated domains, which are known to play a role in constraining gene expression patterns ...
Joanna, Achinger-Kawecka, Susan J, Clark
openaire   +2 more sources

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

A compendium of chromatin interaction maps in the Giant Panda genome

Communications Biology
Elucidating transcriptional regulatory mechanisms within tissues is essential for both physiological and pathological studies of giant panda. Here, we generate transcriptomic profiles and construct 3D genome maps for nine giant panda tissues.
Pengliang Liu, Jiaman Zhang, Kailai Cai, Juan Wang, Hong Liu, Liang Zhang, Wei Xu, Yuliang Liu, Fujun Shen, Rong Hou, Yan Li   +10 more
doaj   +1 more source

The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers

Molecular Oncology, EarlyView.
The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce, Simone Bini, Marialuisa Sponziello, Giorgio Grani, Giulia Fiscon, Paola Paci, Lorenzo Farina, Rosa Falcone, Valentina Maggisano, Sebastiano Filetti, Cosimo Durante, Antonella Verrienti   +11 more
wiley   +1 more source

An integrative 3D-genome database for plants. [PDF]

Plant Commun
High-throughput sequencing technologies have revolutionized studies of 3D genome structures, revealing unprecedented insights into the complexity of genome-wide gene regulation in eukaryotes. To analyze and compare 3D genome structures, we developed a plant 3D-genome database, 3D-GDP, for interspecies comparative functional genomics (http://www.3d-gdp ...
Yang Q, Wu T, Liu M, Zhang X, Sun X, Feng D, Lu Y, Chen X, Hong Y, Ma W, Zhao J.   +10 more
europepmc   +3 more sources

The Functional 3D Organization of Unicellular Genomes [PDF]

Scientific Reports, 2019
AbstractGenome conformation capture techniques permit a systematic investigation into the functional spatial organization of genomes, including functional aspects like assessing the co-localization of sets of genomic elements. For example, the co-localization of genes targeted by a transcription factor (TF) within a transcription factory.
Shay Ben-Elazar, Benny Chor, Zohar Yakhini   +2 more
openaire   +2 more sources

Engineered extracellular vesicles enriched with the miR‐214/199a cluster enhance the efficacy of chemotherapy in ovarian cancer

Molecular Oncology, EarlyView.
Loss of the miR‐214/199a cluster is associated with recurrence in ovarian cancer. Engineered small extracellular vesicles (m214‐sEVs) elevate miR‐214‐3p/miR‐199a‐5p in tumor cells, suppress β‐catenin, TLR4, and YKT6 signaling, reprogram tumor‐derived sEV cargo, reduce chemoresistance and migration, and enhance carboplatin efficacy and survival in ...
Weida Wang, Ayesha Alvero, Yi Qin, Mingjin Wang, Alexandra Fox, Yanfeng Li, Michael Millman, Amy Kemper, Gil Mor, Xian Shuang Liu, Michael Chopp, Zheng Gang Zhang, Yi Zhang   +12 more
wiley   +1 more source

Keratin 19 as a prognostic marker and contributing factor of metastasis and chemoresistance in high‐grade serous ovarian cancer

Molecular Oncology, EarlyView.
Keratin 19 (KRT19) is overexpressed in high‐grade serous ovarian cancer with high levels of Kallikrein‐related peptidases (KLK) 4–7 and is associated with poor survival. In vivo analyses demonstrate that elevated KRT19 increases peritoneal tumour burden.
Sophia Bielesch, Isabel Vogel, Sina Nokodian, Johanna Moeller, Antonia Blechschmidt, Vicky Hecht, Vanessa Kuentzel, Katharina Thiedig, Melissa Schwab, Oliver Schilling, Holger Bronger, Marion Kiechle, Viktor Magdolen, Tobias Dreyer   +13 more
wiley   +1 more source

3D genome organization contributes to genome instability at fragile sites

Nature Communications, 2020
Common fragile sites are regions susceptible to replication stress and are prone to chromosomal instability. Here, the authors, by analyzing the contribution of 3D chromatin organization, identify and characterize a fragility signature and precisely map ...
Dan Sarni, Takayo Sasaki, Michal Irony Tur-Sinai, Karin Miron, Juan Carlos Rivera-Mulia, Brian Magnuson, Mats Ljungman, David M. Gilbert, Batsheva Kerem   +8 more
doaj   +1 more source