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Novel antiadipogenic effect of menadione in 3T3-L1 cells
Chemico-Biological Interactions, 2021Inhibition of adipocyte differentiation can be used as a strategy for preventing adipose tissue expansion and, consequently, for obesity management. Since reactive oxygen species (ROS) have emerged as key modulators of adipogenesis, the effect of menadione (a synthetic form of vitamin K known to induce the increase of intracellular ROS) on 3T3-L1 ...
Melania Iara Funk +3 more
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Glyburide action in cultured 3T3-L1 adipocytes.
Biochemistry international, 1986During adipocyte differentiation of 3T3-L1 cells, glyburide increased the specific activity (mU/mg protein) of glycerol-3-P dehydrogenase (by at least 14-fold) and glutamine synthetase (by 5-fold). The glyburide-mediated increases in enzyme activities were greater in the presence than in the absence of insulin.
MILLER, RE, BHANDARI, B
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Neuroscience Research, 2011
The existence of a cross-talk between nerves and fatty tissue is increasingly recognized. Using co-cultures of dorsal root ganglion (DRG)-derived cells and 3T3-L1 adipocytes, we have previously shown that the presence of fat cells enhances neurite outgrowth and number of synapses.
Joanna, Kosacka +8 more
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The existence of a cross-talk between nerves and fatty tissue is increasingly recognized. Using co-cultures of dorsal root ganglion (DRG)-derived cells and 3T3-L1 adipocytes, we have previously shown that the presence of fat cells enhances neurite outgrowth and number of synapses.
Joanna, Kosacka +8 more
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Journal of Thermal Biology, 2016
Human skin temperature can vary widely depending on anatomical location and ambient temperature. It is also known that local changes in skin and subcutaneous temperature can affect fat metabolism. This study aimed to explore the potential effects of surrounding thermal environment on fat by investigating cell viability, lipid oxidation, and lipid ...
Lin, Xiaofen +6 more
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Human skin temperature can vary widely depending on anatomical location and ambient temperature. It is also known that local changes in skin and subcutaneous temperature can affect fat metabolism. This study aimed to explore the potential effects of surrounding thermal environment on fat by investigating cell viability, lipid oxidation, and lipid ...
Lin, Xiaofen +6 more
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Development of lipoprotein lipase in cultured 3T3-L1 cells
Biochemical and Biophysical Research Communications, 1977Abstract The 3T3-L1 mouse fibroblast resembles an adipocyte after reaching a confluent stage of growth. Lipoprotein lipase activity was released with heparin and was present in acetone-ether extracts of these cells. During the early post-confluent period both activities increased rapidly.
R H, Eckel, W Y, Fujimoto, J D, Brunzell
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Effect of Cortisol on Calpains in the C2C12 and 3T3-L1 Cells
Applied Biochemistry and Biotechnology, 2014The present study was carried out to understand the effect of cortisol on calpain system in the C2C12 and 3T3-L1 adipocyte cells under co-culture system. Cells were co-cultured by using transwell inserts with a 0.4 μm porous membrane to separate C2C12 and 3T3-L1 preadipocyte cells.
Pandurangan, Muthuraman +3 more
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The Effects of AICAR on Adipocyte Differentiation of 3T3-L1 Cells
Biochemical and Biophysical Research Communications, 2001The AMP-activated protein kinase (AMPK) activator, 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR), has been found to inhibit the differentiation of 3T3-L1 adipocytes, if added at an early phase of differentiation. AICAR blocks the expression of the late adipogenic markers, fatty acid synthase and acetyl-CoA carboxylase, and of the transcription ...
S A, Habinowski, L A, Witters
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Glucosamine-induced insulin resistance in 3T3-L1 adipocytes
American Journal of Physiology-Endocrinology and Metabolism, 2000To study molecular mechanisms for glucosamine-induced insulin resistance, we induced complete and reversible insulin resistance in 3T3-L1 adipocytes with glucosamine in a dose- and time-dependent manner (maximal effects at 50 mM glucosamine after 6 h). In these cells, glucosamine impaired insulin-stimulated GLUT-4 translocation.
E, Heart, W S, Choi, C K, Sung
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Regulation of adipocyte differentiation of 3T3-L1 cells by PDZRN3
American Journal of Physiology-Cell Physiology, 2013PDZRN3, a member of the PDZRN (or LNX) family of proteins, is essential for the differentiation of mesenchymal stem cells into myotubes, but it plays an inhibitory role in the differentiation of these cells into osteoblasts. Given that mesenchymal stem cells also differentiate into adipocytes, we examined the possible role of PDZRN3 in adipogenesis in
Takeshi, Honda +2 more
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