Results 71 to 80 of about 345,197 (240)

Characterising the loss-of-function impact of 5’ untranslated region variants in 15,708 individuals

Nature Communications, 2020
Upstream open reading frames (uORFs), located in 5’ untranslated regions, are regulators of downstream protein translation. Here, Whiffin et al. use the genomes of 15,708 individuals in the Genome Aggregation Database (gnomAD) to systematically assess ...
Nicola Whiffin, Konrad J. Karczewski, Xiaolei Zhang, Sonia Chothani, Miriam J. Smith, D. Gareth Evans, Angharad M. Roberts, Nicholas M. Quaife, Sebastian Schafer, Owen Rackham, Jessica Alföldi, Anne H. O’Donnell-Luria, Laurent C. Francioli, Genome Aggregation Database Production Team, Genome Aggregation Database Consortium, Stuart A. Cook, Paul J. R. Barton, Daniel G. MacArthur, James S. Ware   +18 more
doaj   +1 more source

Fine‐Tuned Regulation of mRNA Translation and Transport by STAU2 Condensate Facilitates Neuronal Development and Plasticity

Advanced Science, EarlyView.
STAU2 undergoes phase separation to form dynamic condensates that package target mRNAs and deliver them to the distal ends of growing neuronal dendrites. STAU2 condensates stabilize embedded mRNAs and repress their translation. Synaptic activity bidirectionally remodels STAU2 condensates, coordinating local translation of STAU2‐associated mRNAs ...
Shijing Huang, Yuanyuan Chu, Ruiqian Zhao, Siyu Li, Yu Feng, Hongdan Zheng, Yunli Xie, Tong Wang, Wenyu Wen   +8 more
wiley   +1 more source

The RNA‐Binding Protein PARN Remodeled 3′ UTR Structure Defines Poly(A)‐Loading Sites to Mediate Immunoglobulin Homeostasis

Advanced Science, EarlyView.
ABSTRACT Class Switch Recombination (CSR) is essential for generating high‐affinity antibody isotypes from IgM during adaptive humoral responses. Despite well‐established roles for various transcription factors, whether CSR is subject to dedicated post‐transcriptional control represents a significant gap in knowledge.
Siyuan Sun, Chen Yang, Xiaoyu Wang, Naijing Hu, Shengyao Zhang, Xinchun Li, Yu Chen, Jianan Zhai, Xiangfeng Tang, Wei Lu, Chenxu Lu, Changchang Cao, Weiru Yu, Wenhao Wu, Xiaonan Nie, Fengchao Wang, Bing Fang, Fazheng Ren, Yixuan Li, Juan Chen   +19 more
wiley   +1 more source

UTRdb and UTRsite : a collection of sequences and regulatory motifs of the untranslated regions of eukaryotic mRNAs [PDF]

, 2005
The 5' and 3' untranslated regions of eukaryotic mRNAs play crucial roles in the post-transcriptional regulation of gene expression through the modulation of nucleo-cytoplasmic mRNA transport, translation efficiency, subcellular localization and message ...
MIGNONE, Flavio, G. Grillo, J. Duarte, G. Pesole, F. Licciulli, C. Saccone, S. Liuni, P. J. Kersey, F. Mignone, M. Iacono   +9 more
core   +1 more source

Discovery of regulatory motifs in 5′ untranslated regions using interpretable multi-task learning models

, 2023
The sequence in the 5′ untranslated regions (UTRs) is known to affect mRNA translation rates. However, the underlying regulatory grammar remains elusive.
Ho, Joshua W K, Chu, Athena H Y, Huang, Yuanhua, Wan, Yuk Kei, Zheng, Weizhong, Fong, John H C, Wong, Alan S L   +6 more
core   +1 more source

PlantGFM: A Genomic Foundation Model for Discovery and Creation of Plant Genes

Advanced Science, EarlyView.
A plant genomic foundation model pre‐trained on 12 species enables both accurate gene prediction and de novo gene design. Through AI‐human knowledge screening, seven designed sequences showed transcriptional activity in plants, with two expressing stable proteins—demonstrating the first DNA‐RNA‐protein expression of LLM‐generated genes in plants and ...
Changhao Li, Qizhe Zhang, Hanchen Chen, Kepeng Lin, Chengfang Luo, Mengying Yang, Wei Xu, Fan Yao, Jianbing Yan, Qing‐Yong Yang, Xuehai Hu   +10 more
wiley   +1 more source

Experimental characterization of Cis-acting elements important for translation and transcription in Halophilic Archaea

, 2007
Summary The basal transcription apparatus of archaea is well characterized. However, much less is known about the mechanisms of transcription termination and translation initation.
Jörg Soppa, Oliver Hering, Brenneis, Mariam, Mariam Brenneis, Soppa, Jörg (Prof. Dr.), Brenneis Mariam, Hering, Oliver, Lange, Christian, Christian Lange, Hering Oliver, Soppa Jörg, Lange Christian   +11 more
core   +1 more source

MiR‐940 Suppresses Ferroptosis by Controlling Expression of Key Regulatory Genes

Advanced Science, EarlyView.
A CRISPR‐based screening identified miR‐940 as a critical suppressor of ferroptosis in cancer. By coordinating the downregulation of pro‐ferroptotic genes with the upregulation of GPX4, miR‐940 establishes a regulatory network that protects against ferroptosis and correlates with poor clinical outcomes in distinct cancer entities.
Andrea Kolak, Juliane Tschuck, Stefanie A. I. Weiß, Daniel Kaemena, Karolin Klimm, Ana Galhoz, Larissa Ringelstetter, Myles Fennell, Juliane Merl‐Pham, Anna Artati, Stefanie Strasser, Ralph Garippa, Michael Witting, Hans Zischka, Joel A. Schick, Stefanie M. Hauck, Michael P. Menden, Michelle Vincendeau, Brent R. Stockwell, Kamyar Hadian   +19 more
wiley   +1 more source

Identifying operons and untranslated regions of transcripts using Escherichia coli RNA expression analysis

, 2002
Microarrays traditionally have been used to assay the transcript expression of coding regions of genes. Here, we use Escherichia coli oligonucleotide microarrays to assay transcript expression of both open reading frames (ORFs) and intergenic regions. We
Sergey Stolyar, Carsten Rosenow, Eugene Kolker, David R. Haynor, Brian Tjaden   +4 more
core   +1 more source

Engineered Exosomal miR‐146a‐5p Reprograms BMSC Fate and Restores Mitochondrial Homeostasis in Glucocorticoid‐Induced Osteonecrosis of Femoral Head

Advanced Science, EarlyView.
Exosomal miR‐146a‐5p is identified as a pivotal regulator in steroid‐induced osteonecrosis. Its reduction activates NF‐κB signaling, compromises mitophagy, and disrupts mitochondrial bioenergetics, resulting in autophagic disequilibrium. Engineered exosomes delivering miR‐146a‐5p reinstate mitochondrial function, augment oxidative phosphorylation and ...
Zehui Lv, Xuejie Cai, Yiming Xu, Xingdong Yang, Ruoying Wang, Han Wang, Yixin Bian, Jiawei Xu, Jiao Lu, Lulu Liu, Yingjie Wang, Jibin Song, Bin Feng, Xisheng Weng   +13 more
wiley   +1 more source