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The repair of 5-methylcytosine deamination damage
1993The fact that sites of cytosine methylation in DNA are mutagenic hotspots, and that the genome of higher eukaryotes is thus depleted of the modified sequence CpG is common knowledge — certainly in the field of DNA methylation. The reason why 5-methylcytosine is an endogenous mutagen would also appear to be clear: 5-methylcytosine deaminates to thymine,
K, Wiebauer +3 more
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Photochemical Deamination and Demethylation of 5-Methylcytosine
Chemical Research in Toxicology, 1996Cytosine methylation is believed to play a pivotal role in eucaryotic cellular development as well as in viral latency. We have been investigating chemical mechanisms for the perturbation of methylation patterns, including the effects of ultraviolet radiation. We observed that, upon exposure to UV light, 5-methylcytosine (5mC) was converted to thymine,
E, Privat, L C, Sowers
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Analytical Biochemistry, 1987 A high-performance liquid chromatographic method to separate five major bases (cytosine, thymine, guanine, adenine, and uracil) and three minor methylated bases (5-methylcytosine, N6-methyladenine, and 7-methylguanine) has been developed using a volatile
K P Gopinathan
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Identification of 5-Methylcytosine in Complex Genomes
Methods, 1999Cytosine methylation is attracting new attention for regulatory roles in gene expression and there is an increasing interest in detecting, at a single-base resolution, any 5-methylcytosine in genes from complex genomes. Differential base modification by chemicals followed by PCR-based genomic sequencing procedures can provide the resolution ...
H, Thomassin, E J, Oakeley, T, Grange
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5-Methylcytosine, Gene Regulation, and Cancer
1983Publisher Summary This chapter focuses on 5-methylcytosine, gene regulation, and cancer. The regulation of mammalian gene expression clearly is accomplished by multiple control systems operating at several levels. Some obvious levels of control include chromosome condensation, chromatin structure transcriptional control by repressors and activators ...
A D, Riggs, P A, Jones
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Organization of 5-methylcytosine in chromosomal DNA
Biochemistry, 1978The 5-methylcytosine residues of L-cells have been labeled with [methyl-3H]-L-methionine and their chromatin localization studied using deoxyribonucleases. The kinetics of micrococcal nuclease digestion showed that the methylated cytosine residues are concentrated within regions resistant to nuclease digestion and preferentially missing from those ...
A, Solage, H, Cedar
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5-Methylcytosine-Selective Osmium Oxidation
Nucleosides, Nucleotides and Nucleic Acids, 2007Methylcytosine was efficiently and easily modified through osmium oxidation. Methylcytosine-selective oxidation is applicable to detection of the cytosine methylation status at a specific site of a long sequence using the formation of a bulge structure by hybridization with a guide DNA.
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Phosphopeptides Designed for 5-Methylcytosine Recognition
Biochemistry, 2011An artificial phosphopeptide has been developed through rational design of the interaction with 5-methylcytosine in duplex DNA. The peptide consists of two tandem zinc finger motifs, in one of which the glutamate was replaced with a phosphotyrosine, the phosphotyrosine in the peptide being effective for methylcytosine selectivity of DNA binding.
Akiko, Nomura, Akimitsu, Okamoto
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Proceedings of the National Academy of Sciences of the United States of America
Significance The mechanism and fate by which the dynamic modification of m5C on RNA remain unclear. Here, we report that the canonical 28SrRNA methyltransferase NSUN5 which is epigenetically suppressed by DNA methylation is capable of introducing m5C ...
Ruixin Wu +24 more
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Significance The mechanism and fate by which the dynamic modification of m5C on RNA remain unclear. Here, we report that the canonical 28SrRNA methyltransferase NSUN5 which is epigenetically suppressed by DNA methylation is capable of introducing m5C ...
Ruixin Wu +24 more
semanticscholar +1 more source
5-Methylcytosine and Its Derivatives
2014Epigenetics has undergone an explosion in the past decade. DNA methylation, consisting of the addition of a methyl group at the fifth position of cytosine (5-methylcytosine, 5-mC) in a CpG dinucleotide, is a well-recognized epigenetic mark with important functions in cellular development and pathogenesis.
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