Results 261 to 270 of about 20,996,854 (289)

5-Methylcytosine Analysis by RNA-BisSeq

2018
5-Methylcytosine (m5C) is a posttranscriptional RNA modification identified in both stable and highly abundant tRNAs and rRNAs, and in mRNAs. Many known or novel m5C sites have been validated by using advanced high-throughput techniques combined with next-generation sequencing (NGS), especially RNA bisulfite sequencing (RNA-BisSeq).
Yu-Sheng, Chen, Hai-Li, Ma, Ying, Yang, Wei-Yi, Lai, Bao-Fa, Sun, Yun-Gui, Yang   +5 more
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Structure of 5-methylcytosine hydrochloride

Acta Crystallographica Section C Crystal Structure Communications, 1987
$C_5H_8N_30^+.C1^ -$, $M_r= 161.6$, monoclinic, $P2_1/c$, a=6.431(1), b=16.132(2), c=7.030(1)A, \beta=97.33(1)°, $V=723.29\AA ^3, Z=4, D_x=1.48, D_ m = 1.49 Mg m^{-3}$, \lambda(Cu Ka) = 1.5418 \AA, \mu = 0.423 $mm^{-1}$, F(000) = 336, T = 295 K, R = 0.042 for 1146 observed reflections with I > 3\rho(I). The cytosine base is protonated at N(3).
Padmaja, N, Ramakumar, S, Viswamitra, MA
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The 5-Methylcytosine in DNA of Rats

Gerontologia, 2009
The GC content and amount of 5-methylcytosine (5-MeCyt) have been determined in total DNA from certain organs of male albino rats 1, 12 and 28 months of age. A marked tissue specificity of DNA with respect to the 5-MeCyt content has been revealed. The 5-MeCyt content in DNA decreases with age in the brain, heart and spleen; it does not change in the ...
B.F. Vanyushin, L.E. Nemirovsky, V.V. Klimenko, V.K. Vasiliev, A.N. Belozersky   +4 more
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DNA repair and erasure of 5‐methylcytosine in vertebrates

BioEssays, 2017
DNA methylation plays important roles in development and disease. Yet, only recently has the dynamic nature of this epigenetic mark via oxidation and DNA repair‐mediated demethylation been recognized. A major conceptual challenge to the model that DNA methylation is reversible is the risk of genomic instability, which may come with widespread DNA ...
Lars, Schomacher, Christof, Niehrs
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5-methylcytosine formation in wheat embryo DNA

Biochemical and Biophysical Research Communications, 1980
Summary The methylation of cytosine residues in wheat DNA was studied in isolated embryos during the first 30 hrs of germination. L-Methionine, but also L-serine serve as methyl group donors in vivo . DNA methylation reaches high values after the maximum of DNA replication at 18 hrs.
G, Theiss, H, Follmann
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The cyclobutane dimers of 5-methylcytosine and their deamination products

Photochemical & Photobiological Sciences, 2004
The photochemical reactions of 5-methylcytosine (m(5)C), a minor component of mammalian DNA, have been studied at a concentration of 2 mM in frozen 10 mM aqueous NaCl solution at dry ice temperature (194.5 K). For these studies, low-pressure lamps emitting mainly UVB radiation were used.
Martin D, Shetlar, Vladimir J, Basus, Arnold M, Falick, Anwer, Mujeeb   +3 more
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Ultrastructural localization of 5-methylcytosine on DNA and RNA

Cellular and Molecular Life Sciences, 2017
DNA methylation is the major epigenetic modification and it is involved in the negative regulation of gene expression. Its alteration can lead to neoplastic transformation. Several biomolecular approaches are nowadays used to study this modification on DNA, but also on RNA molecules, which are known to play a role in different biological processes. RNA
Irene Masiello, Marco Biggiogera
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Sequencing 5-methylcytosine residues by the bisulphite method

DNA Sequence, 1996
Measuring patterns of cytosine methylation in genomic DNA is most efficiently accomplished by use of the bisulphite method. This method depends on the large difference in reactivity of cytosines relative to 5-Methyl cytosines in genomic DNA to bisulphite.
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The 5‐methylcytosine content of DNA: Tissue specificity

Journal of Cellular Physiology, 1971
AbstractUsing a new technique for the determination of the extent of DNA methylation by comparing the incorporation of radioactivity from deoxycytidine‐2‐C14 into DNA cytosine and 5‐methylcytosine (5MC), the 5MC content of DNA was found to be a tissue specific property in two systems: (1) mouse tissue culture cell lines, in which the level of 5MC ...
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5-methylcytosine promotes pathogenesis of bladder cancer through stabilizing mRNAs

Nature Cell Biology, 2019
Xin Chen, Ang Li, Bao-Fa Sun, Ying Yang, Yanbo Han, Xun Yuan, Ri-xin Chen, Wen-su Wei, Yanchao Liu, Chunchun Gao, Yu-Sheng Chen, Mengmeng Zhang, Xiao-Dan Ma, Zhuo-wei Liu, Jun‐hang Luo, Cong Lyu, Hailin Wang, Jinbiao Ma, Yong‐Liang Zhao, F. Zhou, Ying Huang, D. Xie, Yungui Yang   +22 more
semanticscholar   +1 more source