Results 161 to 170 of about 121,774 (253)

Development of a Novel Method to Detect AAV Vector Integration. [PDF]

Viruses
Zhang J, Dang TT, Lin TY, Yu X, Pellin D, Tian J, Simmons O, Kou E, Cornetta K, Xiao W.   +9 more
europepmc   +1 more source

Understanding AAV Packaging [PDF]

Biophysical Journal, 2018
openaire   +1 more source

Capture of adeno‐associated viruses from clarified lysate by continuous flow ultracentrifugation: a comparative study of iodixanol and sucrose gradients

Journal of Chemical Technology &Biotechnology, EarlyView.
Abstract BACKGROUND Adeno‐Associated Virus vectors are widely used in gene therapy due to their safety and efficacy. Despite the interest in their therapeutical application, AAV purification remains a significant challenge. Density gradient ultracentrifugation using cesium chloride, sucrose, or iodixanol gradients are common methods for AAV ...
Sabrina Leigheb, Maral Rahimzadeh, Nikolaus Virgolini, Aida Tleuova, Mahbod Mousavian, Astrid Dürauer, Patricia Pereira Aguilar   +6 more
wiley   +1 more source

Self-inactivating AAV-CRISPR at different ages enables sustained amelioration of Huntington's disease deficits in BAC226Q mice. [PDF]

Sci Adv
Dai Y, Abudujielili Z, Ding Y, Huang W, Yin J, Ou L, Hu J, Zheng S, Li C.   +8 more
europepmc   +1 more source

Fat Mass and Obesity‐Associated Protein Contributes to Tumorigenesis and Drug Resistance of Diffuse Large B‐Cell Lymphoma by Suppressing N6‐Methyladenosine Methylation of Myc

The Kaohsiung Journal of Medical Sciences, EarlyView.
ABSTRACT Ibrutinib resistance remains a major obstacle in the treatment of diffuse large B‐cell lymphoma (DLBCL). Fat mass and obesity‐associated protein (FTO) has been implicated in drug resistance through its regulation of N6‐methyladenosine (m6A) modifications; however, whether FTO mediates ibrutinib resistance in DLBCL remains unclear.
Ting‐Ting Lu, Jin‐Hao Chen, Yan‐Fang Wang, Xiao Wu, Hui‐Yun Ni, Qiu‐Rong Zhang   +5 more
wiley   +1 more source

Viral vector-mediated SLC9A6 gene replacement reduces cerebellar motor and molecular abnormalities in the shaker rat model of Christianson syndrome. [PDF]

Hum Mol Genet
Anderson CJ, Figueroa KP, Paul S, Gandelman M, Dansithong W, Katakowski JA, Scoles DR, Pulst SM.   +7 more
europepmc   +1 more source

RBMX Transcriptionally Repressed by EZH2‐Associated H3K27me3 Modification Attenuates Pyroptosis in Renal Ischemia/Reperfusion Injury via Regulating SIRT3/NLRP3 Inflammasome Activation

The Kaohsiung Journal of Medical Sciences, EarlyView.
ABSTRACT Ischemic reperfusion injury (IRI) to kidney is a significant clinical factor in acute kidney injury (AKI). This study aimed to investigate the new role of RNA‐binding motif protein X‐linked (RBMX), a modulator of m6A methylation, in renal IRI and to examine the associated regulatory mechanisms. An in vitro renal IRI model was established using
Yi‐Han Wang, Yan Teng, Fang‐Lan Yao, Shu‐Qi Wei, Yan Li   +4 more
wiley   +1 more source

Neuritin1 Cis-Regulatory Elements Enable Gene Expression Preferentially in Retinal Ganglion Cells. [PDF]

Invest Ophthalmol Vis Sci
Talla V, Koilkonda R, Kinane C, Panchal M, Khuu T, Park KK.   +5 more
europepmc   +1 more source

LncRNA NORAD Enhances Inflammatory Injury in Sepsis‐Associated Acute Lung Damage Through miR‐150‐5p/STAT1‐Dependent NF‐κB Activation

The Kaohsiung Journal of Medical Sciences, EarlyView.
ABSTRACT Acute lung injury (ALI) is a severe complication of sepsis, yet the role of lncRNA NORAD in its pathogenesis remains unclear. Using LPS‐stimulated BEAS‐2B and HBEC3‐KT cells as well as cecal ligation and puncture (CLP)‐induced ALI models in C57BL/6 mice, we found that NORAD expression was markedly upregulated and promoted cell injury ...
Han Liu, Xi‐Xi Chen, Gui‐Hua Wei, Feng Li, Hai‐Yan Zong   +4 more
wiley   +1 more source

Microglia-T cell interactions drive α-synuclein pathology in a Parkinson's disease mouse model. [PDF]

J Neuroinflammation
Ohtake Y, Norikami N, Hong B, Manabe K, Itokazu T, Yamashita T.   +5 more
europepmc   +1 more source