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Acanthamoeba castellanii Can Facilitate Plasmid Transfer Between Environmental Pseudomonas spp. [PDF]
J Basic MicrobiolABSTRACT The conditions in which antimicrobial resistance (AMR) genes are transferred in natural environments are poorly understood. Acanthamoeba castellanii (a cosmopolitan environmental amoeba) feeds on bacteria by phagocytosis, which places the consumed bacteria closely together in a food vacuole (phagosome) of the amoeba.
Sarink MJ +8 more
europepmc +2 more sources
Growth and Encystation of Acanthamoeba castellanii [PDF]
Journal of General Microbiology, 1974 SUMMARY: Complete encystment of Acanthamoeba occurred in peptone-yeast extract-glucose medium supplemented with 50 mM-magnesium chloride. There was an increase in the RNA, protein and DNA contents of the cells which coincided with an increase in the proportion of binucleate amoebae and commitment to encystation, suggesting that encystation could be a ...
A H, Chagla, A J, Griffiths
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Phagocytosis and Pinocytosis in Acanthamoeba castellanii [PDF]
Journal of General Microbiology, 1976 Endocytotic activity of Acanthamoeba trophozoites attenuates once the cells enter stationary phase in liquid culture. Phagocytosis, monitored by the ingestion of polystyrene latex beads, essentially ceases and the uptake of [3H]inulin, known to be mediated by pinocytosis, is reduced by about half.
J A, Chambers, J E, Thompson
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Microorganisms, 2023 Acanthamoeba castellanii genotype T4 is a clinically significant free-living amoeba that causes granulomatous amoebic encephalitis and amoebic keratitis in human beings.
Gerardo Ramírez-Rico +7 more
doaj +1 more source
Acanthamoeba genotype T4 from the UK and Iran and isolation of the T2 genotype from clinical isolates [PDF]
, 2005 The majority of the keratitis-causing Acanthamoeba isolates are genotype T4. In an attempt to determine whether predominance of T4 isolates in Acanthamoeba keratitis is due to greater virulence or greater prevalence of this genotype, Acanthamoeba ...
Alsam +21 more
core +1 more source
Scientific Reports, 2021 Acanthamoeba castellanii, the causative agent of Acanthamoeba keratitis (AK), occurs mainly in contact lens users with poor eye hygiene. The findings of many in vitro studies of AK, as well as the testing of therapeutic drugs, need validation in in vivo ...
Heekyoung Kang +12 more
doaj +1 more source
The cytochromes of Acanthamoeba castellanii [PDF]
Biochemical Journal, 1977 1. Low-temperature difference spectra of gradient-purified mitochondria of Acanthamoeba castellanii reveal the presence of cytochromes b-555, b-562 and c-549, with a-type cytochromes having a broad asymmetrical maximum at 602 nm; these components were also observed in specta of whole cells. 2.
S W, Edwards +3 more
openaire +2 more sources
Chorismate mutase peptide antibody enables specific detection of Acanthamoeba.
PLoS ONE, 2021 Accurate and rapid diagnosis of Acanthamoeba keratitis (AK) is difficult. Although the diagnostic procedure for AK has improved, further development and effective diagnostic tool utilization for AK need to continue.
Hae-Ahm Lee +5 more
doaj +1 more source
Antiamoeboid activity of squamins C–F, cyclooctapeptides from Annona globifora
International Journal for Parasitology: Drugs and Drug Resistance, 2021 Free-living amoebae of Acanthamoeba spp. are causative agents of human infections such as granulomatous amoebic encephalitis (GAE) and Acanthamoeba keratitis (AK). The exploration of innovative chemical entities from natural sources that induce intrinsic
Rubén L. Rodríguez-Expósito +9 more
doaj +1 more source
Induced encystment improves resistance to preservation and storage of Acanthamoeba castellanii [PDF]
, 2008 Several conditions that allow the preservation, storage and rapid, efficient recovery of viable Acanthamoeba castellanii organisms were investigated. The viability of trophozoites (as determined by time to confluence) significantly declined over a period
Campbell, S. J. +3 more
core +1 more source