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Structure of cellulose Acetobacter xylinum
Crystallography Reports, 2003The data are presented on optimization of cellulose synthesis by Acetobacter xylinum (strain VKM V-880) and the structural characteristics of A. xylinum cellulose gel film synthesized during static cultivation. The structural changes caused by the removal of water from gel films are established and the structural organization of macromolecular chains ...
V. V. Klechkovskaya +8 more
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Regulation of Gluconeogenesis in Acetobacter xylinum
European Journal of Biochemistry, 1972Gluconeogenesis in the form of cellulose synthesis from succinate and pyruvate has been studied in resting cells of Acetobacter xylinum. Substrate utilization and cellulose formation occurred at linear rates. At saturating levels of substrate the rate of cellulose formation from succinate was two to three times that from pyruvate.
H, Weinhouse, M, Benziman
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THE LYSIS OF ACETOBACTER XYLINUM
Canadian Journal of Microbiology, 1962Lysis of about 90% of the cells of Acetobacter xylinum by lysozyme may be obtained after 2 hours at pH 8.1 in phosphate–citrate buffer. The cell population is heterogeneous since the remaining 10% of the cells do not lyse, even on repetition of the above treatment.
T. E. Webb, J. Ross Colvin
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Regulation of gluconeogenesis in Acetobacter xylinum by hexoses
Biochemical and Biophysical Research Communications, 1971Abstract Cellulose formation in Acetobacter xylinum occurs at a similar rate when either glucose, fructose or succinate are added as the source of carbon. When either glucose or fructose are added in addition to succinate, cellulose formation from succinate is decreased by 80%.
H, Weinhouse, M, Benziman
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Transglucosidase Activity in Acetobacter xylinum
Nature, 1960CELLS of Acetobacter xylinum are known to produce cellulose. This synthesis has been demonstrated in resting fresh and lyophilized cells1,2. Enzymic synthesis of cellulose by preparations from A. xylinum has been shown with the aid of tracer techniques3,4 and electronmicrography5.
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Biosynthesis of a novel polysaccharide by Acetobacter xylinum
International Journal of Biological Macromolecules, 1994An Acetobacter xylinum adapted to a medium containing N-acetylglucosamine (GlcNAc) has been used to prepare a novel polysaccharide containing residual GlcNAc in cellulose. The maximum amount of incorporation was found to be 4 mol% in cellulose, when a mixed medium containing 1.4% glucose (Glc) and 0.6% GlcNAc was used for the culture of A. xylinum. The
A, Shirai +6 more
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The biosynthesis of cellulose by Acetobacter xylinum and Acetobacter acetigenus
Canadian Journal of Microbiology, 1977The morphological aspects of biosynthesis of cellulose by Acetobacter xylinum and Acetobacter acetigenus were studied by transmission electron microscopy of both freeze-etch replicas and sections of cellulose-free cells in suspension culture before and subsequent to the induction of cellulose synthesis.
J R, Colvin, G G, Leppard
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Phosphorylation coupled to malate oxidation in Acetobacter xylinum
Biochemical and Biophysical Research Communications, 1966Abstract The oxidation of malate to oxaloacetate in A. xylinum has been shown to be irreversible. It is catalyzed by a FAD flavoprotein which is not affected by high concentrations of oxaloacetate and is linked to the cytochrome chain by a vitamin K-like compound ( Benziman and Abeliovitz, 1964 ; Benziman and Galenter, 1964 ; Benziman and ...
M, Benziman, L, Levy
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Endo-β-Glucanase from Acetobacter xylinum : Purification and Characterization
Current Microbiology, 1997A cellulose-producing acetic acid bacterium,Acetobacter xylinum KU-1, abundantly produces an extracellularendo-beta-glucanase (EC 3.2.1.4) in the culture broth. The enzyme was purifiedto homogeneity by DEAE- and CM- Toyopearl 650M ion-exchange chromatography,Butyl-Toyopearl 650M hydrophobic chromatography, and Toyopearl HW-50 gelfiltration.
, Oikawa +4 more
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Synthesis of mannosyl cellobiose diphosphate prenol in Acetobacter xylinum
Archives of Biochemistry and Biophysics, 1980Abstract The enzymatic synthesis of a β-mannosyl (1 → 3) β-glucosyl (1 → 4) α-glucose-1-pyrophosphate-prenol (allylic) by Acetobacter xylinum preparations is described. Glucose pyrophosphate lipid, already known to be formed from UDP-glucose and endogenous phosphate lipid, is demonstrated to accept another glucose from UDP-glucose to give a ...
R O, Couso +3 more
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