Results 51 to 60 of about 2,059,461 (220)

Seminal Plasma Heparin Binding Proteins Improve Semen Quality by Reducing Oxidative Stress during Cryopreservation of Cattle Bull Semen [PDF]

Asian-Australasian Journal of Animal Sciences, 2016
Heparin binding proteins (HBPs) are produced by accessory glands. These are secreted into the seminal fluid, bind to the spermatozoa at the time of ejaculation, favour capacitation, acrosome reaction, and alter the immune system response toward the sperm.
Maulikkumar Patel, Vinod K. Gandotra, Ranjna S. Cheema, Amrit K. Bansal, Ajeet Kumar   +4 more
doaj   +1 more source

Improving ARTs: Microfluidics as a Tool for Sperm Selection

Andrology, EarlyView.
ABSTRACT Sperm selection is a critical step in assisted reproductive technology (ART), as sperm quality directly influences embryo quality. In vivo, the female reproductive tract imposes several physical and physiological barriers that favors only the most optimal spermatozoa reaching the fertilization site.
A. Pérez‐Gómez, A. Urriés, J. L. de Pablo, L. Serrano‐Ramón, S. Sanchez, R. Herrer, J. M. Morrell, C. Malo   +7 more
wiley   +1 more source

In vitro fertilization using sperm activated by ML‐2–3 isolated from Morinda lucida Bentham leaves

Reproductive Medicine and Biology, 2022
Purpose ML‐2–3 is a novel tetracyclic iridoid derived from Morinda lucida Bentham leaves. This compound has anti‐trypanosomal and anti‐leishmanial effects.
Tomoe Ohta, Takuhiro Uto, Yukihiro Shoyama, Maxwell Mamfe Sakyiamah, Alfred Ampomah Appiah, Hiromitsu Tanaka   +5 more
doaj   +1 more source

Osteopontin enhances sperm capacitation and in vitro fertilization efficiency in boars

Journal of Animal Science and Technology, 2022
In this study, we used more reliable experimental materials and methods to detect the effects of osteopontin (OPN) on boar sperm in vitro capacitation, acrosome reaction, and fertilization efficiency.
Yun Chen, Kai Wang, Shouquan Zhang
doaj   +1 more source

Restoration of Motility in Acrosome-Reacted Boar Spermatozoa Induced by the Ionophore 4-Br-A23187. [PDF]

Reprod Domest Anim
ABSTRACT The acrosome reaction (AR) is a calcium‐dependent exocytotic event required for successful fertilization. Calcium ionophores are commonly used to induce AR in vitro; however, this treatment is associated with a significant loss of sperm motility. The aim of this study was to evaluate the recovery of motility in boar spermatozoa after ionophore‐
Martín-Hidalgo D, Calle-Guisado V, Macías-García B, González-Fernández L.   +3 more
europepmc   +2 more sources

ZAN, IZUMO1, and TMEM95 Protein Domains Identify the Physiological Status of the Sperm

Andrology, EarlyView.
ABSTRACT Background Infertility is a growing concern, which has led to an increase in the use of assisted reproductive techniques, involving in vitro sperm capacitation. Nonetheless, the optimal capacitation time remains a matter of debate, and it is unknown whether it has an impact on the localization of key membrane proteins.
Miranda Hernández‐Falcó, Paula Sáez‐Espinosa, Alejandro Romero, Andrea López‐Botella, Laura Robles‐Gómez, María José Gómez‐Torres   +5 more
wiley   +1 more source

IN VITRO BUFFALO SPERM CAPACITATION WITH SPECIAL ATTENTION TO ACROSOME REACTION AND OOCYTE PENETRATION TEST [PDF]

Assiut Veterinary Medical Journal, 1998
In vitro Buffalo sperm capacitation and assessment of acrosome reaction by using Nigrosin-Eosin-Gemisa(NEG) staining technique were performed. Sperm capacitation was done by TALP capacitation media with different concentrations of heparin.
S.H. Shehata
doaj   +1 more source

Male Mice Heterozygous for Protamine 1 and Protamine 2 Are Infertile, Displaying Sperm Damage and Retention of Protamine 2 Precursors, Transition Proteins, and Histones

Andrology, EarlyView.
ABSTRACT Background During spermiogenesis, protamines (PRMs) replace histones to hypercondense and protect sperm DNA. Rodents and primates express two PRMs (PRM1 and PRM2). Alterations of species‐specific PRM ratios are associated with sub‐ or infertility in mice and humans.
Gina Esther Merges, Christoph Wiesejahn, Mar Domingo‐Lopez, Simon Schneider, Andjela Kovacevic, Lena Arévalo, Hubert Schorle   +6 more
wiley   +1 more source

Effects of HFD on sperm acrosome reaction in mice.

, 2015
(A) Status of the mouse sperm acrosome stained with Commassie blue G250 in HFD and CD groups; the arrow indicates the intact acrosome stained with intensive blue. Scale bar = 20 μm.
Ke Xue (296679), Zhide Ding (725787), Weimin Fan (301233), Yali Xu (401789), Yue Liu (292931), Guobao Gu (725786), Yong Fan (212624)   +6 more
core   +1 more source

In vitro Induction and Detection of Acrosomal Exocytosis in Human Spermatozoa

Bio-Protocol, 2020
The acrosome reaction is a highly regulated exocytotic event that primes spermatozoa for successful fertilization. Upon induction, acrosomal exocytosis proceeds via a wave of vesiculation that radiates across the sperm head, destabilizing the acrosomal ...
Shenae Cafe, Amanda Anderson, Brett Nixon   +2 more
doaj   +1 more source