Results 51 to 60 of about 6,799 (175)

The E3 Ligase RNF115 Aggravates Pathological Cardiac Hypertrophy via Ubiquitin‐Mediated Degradation of SPTBN1

Advanced Science, EarlyView.
In response to hypertrophic stimuli, increased c‑JUN phosphorylation upregulates RNF115, leading to SPTBN1 ubiquitination and degradation. which promotes F‑actin depolymerization and YAP activation, driving cardiac hypertrophy. The RNF115 inhibitor DTD effectively suppresses SPTBN1 ubiquitination and cardiac hypertrophy.
Yan Zu, Shiqing Chen, Ji Chen, Liuliu Ruan, Bowen Li, Weijian Chu, Shichen Huang, Dian Yu, Jing Tian, Yuhan Zhao, Yi Han, Xin Tang, Yong Ji   +12 more
wiley   +1 more source

Differential Regulation of Actin Depolymerizing Factor and Cofilin in Response to Alterations in the Actin Monomer Pool [PDF]

Journal of Biological Chemistry, 1997
Myoblasts, transfected with a human gene encoding a beta-actin point mutation, down-regulate expression of actin depolymerizing factor (ADF) and its mRNA. Regulation is posttranscriptional. Expression of cofilin, a structurally similar protein, and profilin, CapG, and tropomodulin is not altered with increasing mutant beta-actin expression.
L S, Minamide, W B, Painter, G, Schevzov, P, Gunning, J R, Bamburg   +4 more
openaire   +2 more sources

Mesenchymal stem cells derived from inflamed dental pulpal and gingival tissue: a potential application for bone formation

Stem Cell Research & Therapy, 2017
Background Chronic periodontal disease is an infectious disease consisting of prolonged inflammation of the supporting tooth tissue and resulting in bone loss.
Laura Tomasello, Rodolfo Mauceri, Antonina Coppola, Maria Pitrone, Giuseppe Pizzo, Giuseppina Campisi, Giuseppe Pizzolanti, Carla Giordano   +7 more
doaj   +1 more source

Molecular and Cellular Hallmarks of Age‐Related Vestibular Hair Cell Degeneration

Advanced Science, EarlyView.
This study utilizes single‐cell RNA‐seq transcriptomes, advanced imaging, and electrophysiology to examine universal and cell‐type‐specific aging signatures of vestibular hair cells. The study shows that impaired hair bundle function is a key driver of age‐related vestibular dysfunction.
Samadhi Kulasooriya, Huizhan Liu, Sarath Vijayakumar, Celia Bloom, Zhenhang Xu, Shu Tu, Benjamin J. Borgmeier, Mi Zhou, Litao Tao, Bechara Kachar, David Z. He   +10 more
wiley   +1 more source

Systemic Nanomechanical Single‐Cell Profiling Reveals Mechanophenotype Transitions Under Therapeutic Perturbation

Advanced Science, EarlyView.
Single‐cell mechanomics demonstrates that pharmacological perturbation changes cytoskeletal and cortical structure, suppressing cancer cell invasiveness. By integrating atomic force microscopy (AFM)‐based cortical measurements with stimulated emission depletion (STED)‐resolved adhesion and cytoskeletal organization, this approach identifies nanoscale ...
Minhee Ku, Jinwon Kwon, Nara Yoon, Hyung Kwon Byeon, Jaemoon Yang   +4 more
wiley   +1 more source

Genetic Ablation and Multi‐Omics Profiling Reveal CEP55 as a Key Driver of Tumorigenesis in Diverse Cancer Models

Advanced Science, EarlyView.
Genetic ablation of Cep55 in Pten‐deficient mouse models delays tumorigenesis. Integrated multi‐omics analyses (proteomics, phosphoproteomics, and spatial transcriptomics) reveal that CEP55 regulates oncogenic signaling (RAS/ERK, PI3K/AKT), integrin/FAK‐mediated adhesion, extracellular matrix (ECM) remodeling, and endocytosis.
Behnam Rashidieh, Yuanhao Yang, Amanda Louise Bain, Phillip Theron, Catharina Vosloo, Sahar Keshvari, Parinaz Ahangar, Brydie Bowden, Simon M. Tria, Ben B. Wang, Sandra Isenmann, Nicholas J. Westra van Holthe, Zherui Xiong, Hani Vu, Sowmya Sharma, Asmerom Sengal, J. Alejandro Lopez, John Finnie, Antonella Papa, Pascal H.G. Duijf, Quan Nguyen, Pirjo M. Apaja, Kum Kum Khanna   +22 more
wiley   +1 more source

Human plasma actin‐depolymerizing factor [PDF]

European Journal of Biochemistry, 1985
The plasma and serum of humans and various animal species exert an actin‐depolymerizing activity. Human actin‐depolymerizing factor (ADF) has been purified by ammonium sulfate fractionation, DEAE‐cellulose and blue‐Sepharose chromatography. It is a single polypeptide of approximately 90 kDa, with a pI between 6.0 and 6.5.
Chaponnier, Christine, Patebex, P., Gabbiani, Giulio   +2 more
openaire   +3 more sources

Targeting polyelectrolyte networks in purulent body fluids to modulate bactericidal properties of some antibiotics

Infection and Drug Resistance, 2018
Robert Bucki,1,* Bonita Durnaś,2,* Marzena Wątek,2,3 Ewelina Piktel,1 Katrina Cruz,4 Przemysław Wolak,2 Paul B Savage,5 Paul A Janmey4 1Department of Microbiological and Nanobiomedical Engineering, Medical University of Białystok, Białystok, 2Department ...
Bucki R, Durnaś B, Wątek M, Piktel E, Cruz K, Wolak P, Savage PB, Janmey PA   +7 more
doaj  

Deciphering Anti‐Cancer Drug Efficacy Through Nanomechanical Vibrations in Living Gastric Cancer Organoids

Advanced Science, EarlyView.
This study developed an efficacy assessment platform that integrates patient‐derived gastric cancer organoids, atomic force microscopy (AFM)‐based nanomechanical vibration detection, deep learning analysis, and organoid mechanical modeling. It detects picomolar drug effects within 0.1 s signal, achieves 97% classification accuracy, and offers non ...
Ting Zhang, Qiubo Chen, Shihai Lan, Liang Zhu, Shuaiyu Liu, Yan Xia, Yalu Zhang, Hanhui Yao, Yongman Liu, Shangquan Wu, Qingchuan Zhang   +10 more
wiley   +1 more source

Cloning and characterization of actin depolymerizing factor from Toxoplasma gondii

Molecular and Biochemical Parasitology, 1997
We determined the predicted amino acid sequence of actin depolymerizing factor (ADF) from Toxoplasma gondii by sequencing the full-length cDNA. T. gondii ADF consists of 118 amino acids (calculated molecular weight 13,400) and shares a high degree of sequence similarity to other low molecular weight actin monomer sequestering proteins, especially ...
M L, Allen, J M, Dobrowolski, H, Muller, L D, Sibley, T E, Mansour   +4 more
openaire   +2 more sources