Results 51 to 60 of about 75,341 (257)
, 2014 Collective dynamics and force generation by cytoskeletal filaments are crucial in many cellular processes. Investigating growth dynamics of a bundle of N independent cytoskeletal filaments pushing against a wall, we show that chemical switching (ATP/GTP ...
Das, Dibyendu +2 more
core +2 more sources
Twirling of actin by myosins II and V observed via polarized TIRF in a modified gliding assay [PDF]
, 2008 The force generated between actin and myosin acts predominantly along the direction of the actin filament, resulting in relative sliding of the thick and thin filaments in muscle or transport of myosin cargos along actin tracks. Previous studies have also detected lateral forces or torques that are generated between actin and myosin, but the origin and
arxiv +1 more source
Relative importance of βcyto- and γcyto-actin in primary mouse embryonic fibroblasts [PDF]
, 2017 The highly homologous β (βcyto) and γ (γcyto) cytoplasmic actins are hypothesized to carry out both redundant and unique essential functions, but studies using targeted gene knockout and siRNA-mediated transcript knockdown to examine βcyto- and γcyto ...
Chamberlain, Christopher M. +5 more
core +1 more source
Novel Actin Cytoskeleton. Actin Tubules.
Cell Structure and Function, 2000 In spores of Dictyostelium discoideum three actin filaments are bundled to form a novel tubular structure and the tubules are then organized into rods. These tubular structures we will term actin tubules. Actin tubules are reconstructed from the supernatant of spore homogenates, while the usual actin filaments were bundled after incubation of ...
Masako Osumi +4 more
openaire +4 more sources
Actin cytoskeleton: Putting a CAP on actin polymerization [PDF]
Current Biology, 2000 Two recent studies have identified a Drosophila homolog of cyclase-associated protein (CAP) as a developmentally important negative regulator of actin polymerization that may also directly mediate signal transduction.
Stevenson, Victoria A. +1 more
openaire +4 more sources
Current Biology, 2021 Robert Insall introduces the cytoskeleton special issue and summarises some recent changes in our view of actin function and regulation.
openaire +2 more sources
Load fluctuations drive actin network growth [PDF]
, 2007 The growth of actin filament networks is a fundamental biological process that drives a variety of cellular and intracellular motions. During motility, eukaryotic cells and intracellular pathogens are propelled by actin networks organized by nucleation-promoting factors, which trigger the formation of nascent filaments off the side of existing ...
arxiv +1 more source
eLife, 2016 New insights into the structure of filaments made of crenactin, a homolog of actin found in archaea, shed light on how the cytoskeleton might have evolved.
Felipe Merino, Stefan Raunser
doaj +1 more source
A method for rapidly screening functionality of actin mutants and tagged actins [PDF]
Biological Procedures Online, 2004 Recombinant production and biochemical analysis of actin mutants has been hampered by the fact that actin has an absolute requirement for the eukaryotic chaperone CCT to reach its native state.
Rommelaere Heidi +4 more
doaj +1 more source
A lipid bound actin meshwork organizes liquid phase separation in model membranes [PDF]
, 2014 The eukaryotic cell membrane is connected to a dense actin rich cortex. We present FCS and STED experiments showing that dense membrane bound actin networks have severe influence on lipid phase separation. A minimal actin cortex was bound to a supported lipid bilayer via biotinylated lipid streptavidin complexes (pinning sites).
arxiv +1 more source