Results 51 to 60 of about 243,847 (379)

Purification of a cortical complex containing two unconventional actins from Acanthamoeba by affinity chromatography on profilin-agarose

Journal of Cell Biology, 1994
We identified four polypeptides of 47, 44, 40, and 35 kD that bind to profilin-Sepharose and elute with high salt. When purified by conventional chromatography using an antibody to the 47-kD polypeptide, these four polypeptides copurified as a ...
Laura M. Machesky, Simon J Atkinson, C. Ampe, Joël Vandekerckhove, T. D. Pollard   +4 more
semanticscholar   +1 more source

A method for rapidly screening functionality of actin mutants and tagged actins [PDF]

Biological Procedures Online, 2004
Recombinant production and biochemical analysis of actin mutants has been hampered by the fact that actin has an absolute requirement for the eukaryotic chaperone CCT to reach its native state.
Rommelaere Heidi, Waterschoot Davy, Neirynck Katrien, Vandekerckhove Joël, Ampe Christophe   +4 more
doaj   +1 more source

Concanavalin A-induced cap formation in rat ascites hepatoma cells (AH 7974) and the interaction of cytoplasmic proteins with plasma membranes. [PDF]

, 1987
Concanavalin A (Con A) induced cap formation in rat ascites hepatoma cells (AH7974). In these Con A-treated cells, the association of cytoplasmic proteins with cell membranes was suggested by observing their Triton shells.
Moromizato, Yasunori, Sasaki, Junzo, Watanabe, Sadahiro   +2 more
core   +1 more source

Orchestrated control of filaggrin-actin scaffolds underpins cornification. [PDF]

, 2018
Epidermal stratification critically depends on keratinocyte differentiation and programmed death by cornification, leading to formation of a protective skin barrier.
Bernardino de la Serna, Jorge, Colin-York, Huw, Eggeling, Christian, Fritzsche, Marco, Gutowska-Owsiak, Danuta, Leeming, Michael, Naeem, Aishath, Ogg, Graham, O’Shaughnessy, Ryan, Podobas, Ewa Izabela   +9 more
core   +6 more sources

The mother of all actins?

eLife, 2016
New insights into the structure of filaments made of crenactin, a homolog of actin found in archaea, shed light on how the cytoskeleton might have evolved.
Felipe Merino, Stefan Raunser
doaj   +1 more source

A novel microfluidic flow-cytometry for counting numbers of single-cell β-actins

Nanotechnology and Precision Engineering, 2020
As a house keeping protein with stable expressions, β-actin is used as a loading control in normalization of western blotting. However, the actual numbers of β-actins at the single-cell level remain elusive.
Lixing Liu, Beiyuan Fan, Hongyu Yang, Deyong Chen, Shuang Zhang, Junbo Wang, Jian Chen   +6 more
doaj   +1 more source

Septin filament organization in Saccharomyces cerevisiae. [PDF]

, 2012
Septins are a family of GTP-binding, membrane-interacting cytoskeletal proteins, highly conserved and essential in all eukaryotes (with the exception of plants). Septins play important roles in a number of cellular events that involve membrane remodeling
Bertin, Aurélie, NOGALES, Eva
core   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

TNIK Regulates Cytoskeletal Organization to Promote Focal Adhesion Turnover and Mitosis in Lung Adenocarcinoma

Frontiers in Bioscience-Landmark
Background: Lung cancer is the primary cause of cancer-related mortality, but the molecular mechanisms behind this malignancy remain unclear.
Yao Li, Meng-yao Song, Xing Hu, Xue-hua Sun, Tao Zhang, Lu Zhang, Ying-xiong Wang, Qian Zhang, Chun-dong Zhang, Lian Zhang   +9 more
doaj   +1 more source

Partially Redundant Actin Genes in Chlamydomonas Control Transition Zone Organization and Flagellum-Directed Traffic

Cell Reports, 2019
Summary: The unicellular green alga Chlamydomonas reinhardtii is a biflagellated cell with two actin genes: one encoding a conventional actin (IDA5) and the other encoding a divergent novel actin-like protein (NAP1).
Brittany Jack, David M. Mueller, Ann C. Fee, Ashley L. Tetlow, Prachee Avasthi   +4 more
doaj   +1 more source