Results 1 to 10 of about 8,825 (179)

Improvement of recombinant ADAMTS13 production through a more optimal signal peptide or an N‐terminal fusion protein

Journal of Thrombosis and Haemostasis, 2022
Recombinant human ADAMTS13 (rADAMTS13) is a key protein in fundamental research for investigating its mode of action and the pathophysiology of thrombotic thrombocytopenic purpura (TTP). However, the expression of rADAMTS13 is quite low in mammalian cells, which makes the production of the protein time-consuming and labor-intensive.We aimed at ...
Kadri Kangro, Elien Roose, Aline Vandenbulcke   +2 more
exaly   +4 more sources

Decreased protein C activity, lower ADAMTS13 antigen and free protein S levels accompanied by unchanged thrombin generation potential in hospitalized COVID-19 patients

Thrombosis Research, 2023
COVID-19 is associated with an increased thromboembolic risk. However, the mechanisms triggering clot formation in those patients remain unknown.In 118 adult Caucasian severe but non-critically ill COVID-19 patients (median age 58 years; 73 % men) and 46 controls, we analyzed in vitro plasma thrombin generation profile (calibrated automated thrombogram
Tomasz Stachura, Krzysztof Sładek
exaly   +4 more sources

Local Elongation of Endothelial Cell-anchored von Willebrand Factor Strings Precedes ADAMTS13 Protein-mediated Proteolysis [PDF]

Journal of Biological Chemistry, 2011
Platelet-decorated von Willebrand factor (VWF) strings anchored to the endothelial surface are rapidly cleaved by ADAMTS13. Individual VWF string characteristics such as number, location, and auxiliary features of the ADAMTS13 cleavage sites were explored here using imaging and computing software.
Susana Rocha, Hendrik B Feys, Simon F De Meyer   +2 more
exaly   +4 more sources

Generation and validation of small ADAMTS13 fragments for epitope mapping of anti‐ADAMTS13 autoantibodies in immune‐mediated thrombotic thrombocytopenic purpura

Research and Practice in Thrombosis and Haemostasis, 2020
Background In immune‐mediated thrombotic thrombocytopenic purpura (iTTP), patients develop an immune response against the multidomain enzyme ADAMTS13.
Kadri Kangro, Elien Roose, An‐Sofie Schelpe, Edwige Tellier, Gilles Kaplanski, Jan Voorberg, Simon F. De Meyer, Andres Männik, Karen Vanhoorelbeke   +8 more
doaj   +2 more sources

Pathogenicity of Anti-ADAMTS13 Autoantibodies in Acquired Thrombotic Thrombocytopenic Purpura [PDF]

EBioMedicine, 2015
BACKGROUND: Acquired thrombotic thrombocytopenic purpura (TTP) is an autoimmune disease in which anti-ADAMTS13 autoantibodies cause severe enzyme deficiency.
Rens de Groot, Marie Scully, James T B Crawley   +2 more
exaly   +4 more sources

An IAP retrotransposon in the mouse ADAMTS13 gene creates ADAMTS13 variant proteins that are less effective in cleaving von Willebrand factor multimers

Blood, 2007
AbstractSevere deficiency of ADAMTS13, a von Willebrand factor (VWF)–cleaving metalloprotease, causes thrombotic thrombocytopenic purpura. When analyzed with VWF multimers, but not with an abbreviated VWF peptide (VWF73) as the substrate, the plasma ADAMTS13 activity levels of mouse strains segregated into a high and a low group that differed by ...
Eric E Bouhassira, Han-Mou Tsai
exaly   +4 more sources

ADAMTS13 in Bothrops lanceolatus snakebite envenoming: Crude venom-induced reduction of in vitro enzymatic activity and clinical correlation in snakebite patients. [PDF]

PLoS Neglected Tropical Diseases
BackgroundEnvenoming by Bothrops lanceolatus, a viperid endemic to Martinique an island in the Lesser Antilles, induces a unique clinical manifestation, i.e., thrombosis. Pathophysiological signaling leading to thrombotic events remains poorly understood.
Jonathan Florentin, Caroline Rapon, Olivier Pierre-Louis, Fatima Radouani, Prisca Jalta, Pascal Fuseau, Hatem Kallel, Remi Neviere, Dabor Resiere   +8 more
doaj   +2 more sources

Von Willebrand Factor and ADAMTS13 in Relation to Major Adverse Limb Events in Peripheral Artery Disease [PDF]

Vascular Specialist International
Purpose: This study aimed to investigate the relationship between the two biomarkers, von Willebrand factor (VWF) and ADAMTS13, and major adverse limb events (MALE) in patients with peripheral artery disease (PAD). Materials and Methods : After obtaining
Noa Agid, Peter Andrisani, Hamzah Khan, Mohammad Qadura, Colin A. Kretz, Vinai C. Bhagirath   +5 more
doaj   +2 more sources

Optimization of plasma-based BioID identifies plasminogen as a ligand of ADAMTS13

Scientific Reports
ADAMTS13, a disintegrin and metalloprotease with a thrombospondin type 1 motif, member 13, regulates the length of Von Willebrand factor (VWF) multimers and their platelet-binding activity. ADAMTS13 is constitutively secreted as an active protease and is
Hasam Madarati, Veronica DeYoung, Kanwal Singh, Taylor Sparring, Andrew C. Kwong, James C. Fredenburgh, Cherie Teney, Marlys L. Koschinsky, Michael B. Boffa, Jeffrey I. Weitz, Colin A. Kretz   +10 more
doaj   +2 more sources

Overview of ADAMTS13 Protein in Diagnosis and Patient Management of TTP [PDF]

Documenta Haematologica
The accurate diagnosis and effective management of Thrombotic Thrombocytopenic Purpura (TTP), a rare but life-threatening hematologic disorder, rely critically on the assessment of ADAMTS13 activity.
Georgiana GHERGHE, Valentina USCATESCU, Delia Codruta POPA, Diana PREDA, Didona VASILACHE, Mihai Emanuel HIMCINSCHI, Horia SANDU, Daniel CORIU   +7 more
doaj   +2 more sources