Results 71 to 80 of about 42,221 (215)

Programming Next‐Generation Synthetic Biosensors by Genetic Circuit Design

Advanced Science, Volume 13, Issue 14, 9 March 2026.
Synthetic biology enables genetic circuit‐based biosensing to detect diverse targets, process signals, and transduce them into readable outputs or intracellular regulatory activities. However, field deployment and real‐world application of such synthetic biosensors face considerable challenges in sensitivity, specificity, speed, stability, and ...
Yuanli Gao, Cheng Huang, Jiaxuan Deng, Lei Wang, Baojun Wang   +4 more
wiley   +1 more source

Systematic identification and characterization of high efficiency Cas9 guide RNAs for therapeutic targeting of ADAR.

PLoS ONE
Therapeutic targeting of the adenosine deaminase ADAR has great potential in cancer and other indications; however, it remains unclear what approach can enable effective and selective therapeutic inhibition.
Benjamin G Gowen, Kory Melton, Weng In Leong, Prachi Khekare, Shannon McCawley, Jean Chan, Pierre Boivin, Vihasi Jani, Aaron J Cantor, Akshay Tambe, Mary Haak-Frendscho, Mary J Janatpour, Spencer C Wei   +12 more
doaj   +1 more source

Utilizing AAV-mediated LEAPER 2.0 for programmable RNA editing in non-human primates and nonsense mutation correction in humanized Hurler syndrome mice

Genome Biology, 2023
Background The endogenous adenosine deaminases acting on RNA (ADAR) have been harnessed to facilitate precise adenosine-to-inosine editing on RNAs.
Zongyi Yi, Yanxia Zhao, Zexuan Yi, Yongjian Zhang, Gangbin Tang, Xiaoxue Zhang, Huixian Tang, Wei Zhang, Ying Zhao, Huayuan Xu, Yuyang Nie, Xueqing Sun, Lijun Xing, Lian Dai, Pengfei Yuan, Wensheng Wei   +15 more
doaj   +1 more source

cGAS inhibitor IMSB301 modifies interferon signalling in peripheral mononuclear cells of SAMHD1 genetic interferonopathy in vitro

Clinical &Translational Immunology, Volume 15, Issue 3, 2026.
Depicts the pathways and mechanisms of the drug. Abstract Objectives Aicardi–Goutières syndrome (AGS) is a rare genetic interferonopathy because of aberrant DNA or RNA metabolism that lacks effective disease modifying therapies. Methods Single‐cell RNA sequencing was performed on peripheral blood mononuclear cells (PBMCs) obtained from a patient with ...
Velda X Han, Jessica P Hayes, Lijun Sun, Teresa Mooneyham, Michelle Lorentzos, Hiroya Nishida, Markus J Hofer, Brian Gloss, Ruwani Dissanayake, Xianzhong Lau, Shekeeb S Mohammad, Shrujna Patel, Russell C Dale   +12 more
wiley   +1 more source

Unbiased Identification of trans Regulators of ADAR and A-to-I RNA Editing

Cell Reports, 2020
Summary: Adenosine-to-inosine RNA editing is catalyzed by adenosine deaminase acting on RNA (ADAR) enzymes that deaminate adenosine to inosine. Although many RNA editing sites are known, few trans regulators have been identified.
Emily C. Freund, Anne L. Sapiro, Qin Li, Sandra Linder, James J. Moresco, John R. Yates, III, Jin Billy Li   +6 more
doaj   +1 more source

The Fatty Acid Transporter CD36 Mediates Uptake, Biodistribution, and Cardioprotection by Small Extracellular Vesicles From HEK293 Cells

Journal of Extracellular Vesicles, Volume 15, Issue 3, March 2026.
ABSTRACT Extracellular vesicles (EVs) are nanoscale carriers of bioactive molecules that mediate intercellular communication. Small EVs (sEVs) have shown cardioprotective effects in models of myocardial infarction (MI), but their uptake and biodistribution remain incompletely understood.
Elias Sulaiman, Derek M. Yellon, Sean M. Davidson   +2 more
wiley   +1 more source

How do ADARs bind RNA? New protein‐RNA structures illuminate substrate recognition by the RNA editing ADARs [PDF]

BioEssays, 2017
Deamination of adenosine in RNA to form inosine has wide ranging consequences on RNA function including amino acid substitution to give proteins not encoded in the genome. What determines which adenosines in an mRNA are subject to this modification reaction?
Thomas, Justin M, Beal, Peter A
openaire   +4 more sources

RNA‐Based Therapies for Inherited Metabolic Disorders

Journal of Inherited Metabolic Disease, Volume 49, Issue 2, March 2026.
ABSTRACT Inherited metabolic disorders (IMDs) are a diverse and complex group of genetic conditions resulting from deficiencies in enzymes, transporters, or cofactors. These deficiencies lead to metabolic dysfunction and severe clinical consequences. Despite significant progress in understanding their molecular basis, treatment options remain limited ...
Reddy Sreekanth Vootukuri, Sonam Gurung, Roopkatha Ghosh, Philippa B. Mills, Julien Baruteau, Haiyan Zhou   +5 more
wiley   +1 more source

Deep transcriptome profiling reveals limited conservation of A-to-I RNA editing in Xenopus

BMC Biology, 2023
Background Xenopus has served as a valuable model system for biomedical research over the past decades. Notably, ADAR was first detected in frog oocytes and embryos as an activity that unwinds RNA duplexes. However, the scope of A-to-I RNA editing by the
Tram Anh Nguyen, Jia Wei Joel Heng, Yan Ting Ng, Rui Sun, Shira Fisher, Gokce Oguz, Pornchai Kaewsapsak, Shifeng Xue, Bruno Reversade, Adaikalavan Ramasamy, Eli Eisenberg, Meng How Tan   +11 more
doaj   +1 more source

Solution structure of the N-terminal dsRBD of Drosophila ADAR and interaction studies with RNA [PDF]

, 2012
Adenosine deaminases that act on RNA (ADAR) catalyze adenosine to inosine (A-to-I) editing in double-stranded RNA (dsRNA) substrates. Inosine is read as guanosine by the translation machinery; therefore A-to-I editing events in coding sequences may ...
Barraud, Barraud, Barraud, Bass, Bhalla, Blaszczyk, Bratt, Bret S.E. Heale, Brunger, Brunger, Burns, Bycroft, Chang, Cho, DeLano, Dominguez, Duss, Eggington, Fierro-Monti, Frédéric H.-T. Allain, Gallo, Gan, Goddard, Graveley, Guntert, Guntert, Herrmann, Herrmann, Higuchi, Hood, Hoopengardner, Keegan, Keegan, Kharrat, Krovat, Laskowski, Lehmann, Leulliot, Lomeli, Maas, Macbeth, Mary A. O’Connell, Nishikura, Palladino, Palladino, Paro, Pierre Barraud, Ramos, Ryter, Saunders, Sommer, Stapleton, Stefl, Stefl, Stefl, Stephens, Valente, Wang, Wu, Yang   +59 more
core   +4 more sources