Results 241 to 250 of about 34,085 (286)

Optimizations of Cas12a‐ and Cas12i‐based adenine base editors for efficient precision editing in the plant genome

Journal of Integrative Plant Biology, EarlyView.
A strategy coupling high‐activity nucleases with dimeric TadA‐8e optimizes plant Cas12‐based adenine base editors, boosts editing efficiency, and provides precise editors for crop breeding and genomics research.
Xiaoshuang Liu, Shan Jin, Zhi Xiao, Chong Ma, Qing Wang, Huanhuan Wang, Ruohan Zhou, Dongfang Gu, Rongfang Xu, Ruiying Qin, Juan Li, Pengcheng Wei   +11 more
wiley   +1 more source

Towards an integrated molecular understanding of plant hormones

Journal of Integrative Plant Biology, EarlyView.
This review offers a comprehensive overview of the nine plant hormones, delving into their biosynthesis, transport, signaling and crosstalk mechanisms. Because the complexity of plant hormonal control goes beyond these core elements, additional specific features are discussed. Lastly, this review highlights how fundamental insights drive hormonal‐based
Louise Vilain, Marie‐Laure Fauconnier, Manon Genva   +2 more
wiley   +1 more source

Advances in CRISPR Base Editing: From Molecular Evolution to Therapeutic Applications in Genomic Medicine. [PDF]

J Cell Mol Med
Aliciaslan M, Erbasan E, Erendor F, Sanlioglu S.   +3 more
europepmc   +1 more source

Enhancing CRISPR‐Cas12a base editing in plants with LbCas12a variants and introns

Journal of Integrative Plant Biology, EarlyView.
Intron optimization of LbCas12a‐RRV improves cytosine and adenine base editing efficiency in plants, supports multiplexed and double‐strand break‐free genome modification, and expands precise genome engineering tools for crop breeding and plant functional research. ABSTRACT Cytosine base editors (CBEs) and adenine base editors (ABEs) are powerful tools
Yanhao Cheng, Gen Li, Man Zhou, Rushil Mandlik, Doris Wang, Yiping Qi   +5 more
wiley   +1 more source

Optimized TadA‐derived base editors efficiently manipulate mRNA splicing by A‐to‐G and C‐to‐K editing in potato

Journal of Integrative Plant Biology, EarlyView.
Two optimized TadA‐derived base editors efficiently generate diverse splicing variants by targeting specific splice sites in potato. ABSTRACT Pre‐messenger RNA (pre‐mRNA) splicing is a critical mechanism for post‐transcriptional regulation in plants. Through alternative splicing, plants produce diverse transcriptomes and proteomes that finely regulate ...
Kaiyuan Chen, Yan Zhang, Jiuzhou Deng, Lumin Zhang, Huiying Zhou, Yaxin Duan, Enle Xiao, Guangtao Zhu, Chunzhi Zhang   +8 more
wiley   +1 more source

ABE9 fused to SpRY Cas9 nickase enables precise generation of bystander free mouse models. [PDF]

Sci Rep
Ong JK, Bhunia S, Hilbert B, Kirschner V, Duglosz S, Zimmermann F, Freichel M, Cornean A.   +7 more
europepmc   +1 more source

Genomic variation drives plant flavor diversification

Journal of Integrative Plant Biology, EarlyView.
This review explains how genomic variation shapes plant flavor by altering the biosynthetic and regulatory pathways of key attributes like sweetness, acidity, bitterness, piquancy, astringency, and aroma. It also discusses how multi‐omics, AI‐assisted breeding, and gene editing can translate this knowledge into plants with improved flavor, nutrition ...
Huimin Hu, Yanwei Hao, Yiwei Zhou, Xia Zhang, Rui Xia, Pan Liao   +5 more
wiley   +1 more source

sgRNA amount is a limiting factor in adenine base editing using RNA LNPs. [PDF]

Mol Ther Nucleic Acids
Birkenshaw A, Thomson T, Truong MP, Komaki Y, Ramsden N, Timpano A, Huang C, Blakney AK, Kurek DZ, Kulkarni J, Ross CJD.   +10 more
europepmc   +1 more source

The ZmRACK1–ZmCDPK7–ZmAPX1 module regulates plant antiviral immunity

Journal of Integrative Plant Biology, EarlyView.
In maize, ZmRACK1 acts as a scaffold protein that bridges the cyclin‐dependent kinase ZmCDPK7 and the ascorbate peroxidase ZmAPX1, promoting ZmAPX1 activity to mitigate MCMV infection. Upon viral infection, P31 obstructs formation of the ZmRACK1‐ZmCDPK7‐ZmAPX1 complex, inhibiting ZmCDPK7‐mediated ZmAPX1 activity and promoting reactive oxygen species ...
Yuyang Zhang, Niannian Li, Qiongqiong Wang, Yingchao Sun, Yajuan Shi, Lianwei Yu, Xiaoyu Han, Xiaoxi Feng, Zaifeng Fan, Linlin Chen, Honglian Li, Xue Yang, Hongxia Yuan, Yan Shi   +13 more
wiley   +1 more source

Editing and synthetic engineering of chloroplast genomes. [PDF]

Plant Commun
Fu J, Zhang J.
europepmc   +1 more source