Results 241 to 250 of about 19,445 (257)

Hypercompact adenine base editors based on transposase B guided by engineered RNA.

Nature chemical biology, 2022
Transposon-associated transposase B (TnpB) is deemed an ancestral protein for type V, Cas12 family members, and the closest ancestor to UnCas12f1. Previously, we reported a set of engineered guide RNAs supporting high indel efficiency for Cas12f1 in human cells.
Do Yon, Kim, Yuhee, Chung, Yujin, Lee, Dongmin, Jeong, Kwang-Hyun, Park, Hyun Jung, Chin, Jeong Mi, Lee, Seyeon, Park, Sumin, Ko, Jeong-Heon, Ko, Yong-Sam, Kim   +10 more
openaire   +2 more sources

Precision genome editing using cytosine and adenine base editors in mammalian cells

Nature Protocols, 2021
Genome editing has transformed the life sciences and has exciting prospects for use in treating genetic diseases. Our laboratory developed base editing to enable precise and efficient genome editing while minimizing undesired byproducts and toxicity associated with double-stranded DNA breaks.
Tony P. Huang, Gregory A. Newby, David R. Liu   +2 more
openaire   +2 more sources

An AI-designed adenine base editor

Abstract Adenine base editors (ABEs) allow the efficient programmable conversion of adenine to guanine without causing DNA double strand breaks. Previous ABEs were generated by multiple rounds of directed evolution or derived by rational design based on the evolved ones.
Ye Yuan, Yang Chen, Rui Liu, Gula Que, Yina Yuan, Guipeng Li   +5 more
openaire   +1 more source

Off-target effects of cytidine base editor and adenine base editor: What can we do?

Journal of Genetics and Genomics, 2019
Abstract Base editors (BEs) is a powerful tool that could edit the genome efficiently without inducing DNA double-strand breaks (DSBs). Recently, off-target DNA and RNA mutations of BEs were found, rasing concerns about its precision and safety. Here we discuss some strategies that might eliminate off-target effects of BEs.
Puping Liang, Jinkun Wen, Junjiu Huang
openaire   +2 more sources

Adenine base editor excels at fixing point mutations

C&EN Global Enterprise, 2017
Many diseases are caused by single-base mutations in genomic DNA. Scientists have long searched for methods to correct such mutations to treat these genetic diseases. Last year, David R. Liu of Harvard University and coworkers developed a technique called base editing that fixes some single-base mutations.
openaire   +1 more source

Programmable A-to-Y base editing by fusing an adenine base editor with an N-methylpurine DNA glycosylase

Nature Biotechnology, 2023
Huawei Tong, Chunlong Xu, Hui Yang
exaly  

Artificial evolution of OsEPSPS through an improved dual cytosine and adenine base editor generated a novel allele conferring rice glyphosate tolerance

Journal of Integrative Plant Biology, 2023
Shaoya Li, Jingying Li, Yubing He
exaly  

Hypercompact adenine base editors based on a Cas12f variant guided by engineered RNA

Nature Chemical Biology, 2022
Do Yon Kim, Yuhee Chung, Yujin Lee, Dongmin Jeong, Kwang-Hyun Park, Hyun Jung Chin, Jeong Mi Lee, Seyeon Park, Sumin Ko, Jeong-Heon Ko, Yong-Sam Kim   +10 more
openaire   +1 more source

Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity

Nature Biotechnology, 2022
Alvin Hsu, Nicholas Krasnow, Amber N Mcelroy   +2 more
exaly  

A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing

Nature Biotechnology, 2020
Julian Grunewald, Ronghao Zhou, Caleb A Lareau   +2 more
exaly