Results 11 to 20 of about 396,665 (375)

Charge-to-Alanine Mutagenesis of the Adeno-Associated Virus Type 2 Rep78/68 Proteins Yields Temperature-Sensitive and Magnesium-Dependent Variants

Virology, 1999
The adeno-associated virus (AAV) replication proteins Rep78 and Rep68 regulate viral gene expression and DNA amplification. Their effects on both processes suggest that they play roles in all phases of the virus life cycle. We have investigated Rep protein phosphorylation to determine if this modification might alter Rep function. All four Rep proteins
Denise Gavin, Samuel Young, Weidong Xiao, Brenda Temple, Corinne R. Abernathy, Daniel J. Pereira, Nicholas Muzyczka, R. Jude Samulski   +7 more
openalex   +5 more sources

Adeno-associated virus and hepatocytes: frenemies? [PDF]

Blood Adv
Pierce GF.
europepmc   +3 more sources

Advanced biomanufacturing and evaluation of adeno-associated virus

Journal of Biological Engineering
Recombinant adeno-associated virus (rAAV) has been developed as a safe and effective gene delivery vehicle to treat rare genetic diseases. This study aimed to establish a novel biomanufacturing process to achieve high production and purification of ...
Kai Chen, Seulhee Kim, Siying Yang, Tanvi Varadkar, Zhuoxin Zora Zhou, Jiashuai Zhang, Lufang Zhou, Xiaoguang Margaret Liu   +7 more
doaj   +3 more sources

Sclerosing Sialadenitis Is Associated With Salivary Gland Hypofunction and a Unique Gene Expression Profile in Sjögren’s Syndrome

Frontiers in Immunology, 2021
PurposeTo develop a novel method to quantify the amount of fibrosis in the salivary gland and to investigate the relationship between fibrosis and specific symptoms associated with Sjögren’s syndrome (SS) using this method.Materials and MethodsParaffin ...
Hongen Yin, Thomas J. F. Pranzatelli, Benjamin N. French, Nan Zhang, Blake M. Warner, John A. Chiorini, NIDCD/NIDCR Genomics and Computational Biology Core   +6 more
doaj   +1 more source

Advancing Luciferase-Based Antibody Immunoassays to Next-Generation Mix and Read Testing

Biosensors, 2023
Antibody measurements play a central role in the diagnosis of many autoimmune and infectious diseases. One antibody detection technology, Luciferase Immunoprecipitation Systems (LIPS), utilizes genetically encoded recombinant luciferase antigen fusion ...
Peter D. Burbelo, Youngmi Ji, Michael J. Iadarola   +2 more
doaj   +1 more source

Adeno-associated virus type 2 in US children with acute severe hepatitis

Nature, 2023
As of August 2022, clusters of acute severe hepatitis of unknown aetiology in children have been reported from 35 countries, including the USA^ 1 , 2 .
V. Servellita, Alicia Sotomayor Gonzalez, D. Lamson, A. Foresythe, H. Huh, A. Bazinet, N. Bergman, R. Bull, Karla Y. Garcia, J. S. Goodrich, Sean P Lovett, Kisha Parker, D. Radune, April Hatada, C. Pan, K. Rizzo, J. B. Bertumen, Christina Morales, P. Oluniyi, Jenny Nguyen, Jessica Tan, D. Stryke, Rayah Jaber, Matthew T. Leslie, Zin Lyons, H. Hedman, U. Parashar, Maureen Sullivan, Kelly Wroblewski, M. Oberste, J. Tate, J. M. Baker, D. Sugerman, Caelin C. Potts, Xiaoyan Lu, P. Chhabra, Olivia Neha Jordan E. Brandi Anita Rachel Everardo Mary E Almendares Balachandran Cates Freeman Kambhampati , O. Almendares, Neha Balachandran, J. Cates, Brandi Freeman, Anita K Kambhampati, Rachel L. Marine, Everardo Vega, M. Wikswo, Erin McKeever, David Kelly, J. Boom, Leila C. Sahni, J. Englund, E. Klein, M. Staat, Maeve Marsh, Erin Ricketts, J. Clayton, N. Cano, M. Oseguera, R. Blattner, L. A. Ingram, Henry Shiau, W. Britt, L. H. Gutierrez Sanchez, C. Ciric, C. Rostad, J. Vinjé, H. Kirking, D. Wadford, R. T. Raborn, K. St. George, C. Chiu   +69 more
semanticscholar   +1 more source

Immunogenicity of Recombinant Adeno-Associated Virus (AAV) Vectors for Gene Transfer

BioDrugs, 2023
Recombinant adeno-associated viruses (AAVs) have emerged as promising gene delivery vehicles resulting in three US Food and Drug Administration (FDA) and one European Medicines Agency (EMA)-approved AAV-based gene therapies.
Motahareh Arjomandnejad, I. Dasgupta, T. Flotte, Allison M. Keeler   +3 more
semanticscholar   +1 more source

Correction of LAMP3-associated salivary gland hypofunction by aquaporin gene therapy

Scientific Reports, 2022
Sjögren’s disease (SjD) is a chronic autoimmune sialadenitis resulting in salivary gland hypofunction with dry mouth symptom. Previous studies showed that lysosome-associated membrane protein 3 (LAMP3) overexpression is involved in the development of ...
Hiroyuki Nakamura, Tsutomu Tanaka, Changyu Zheng, Sandra A. Afione, Blake M. Warner, Masayuki Noguchi, Tatsuya Atsumi, John A. Chiorini   +7 more
doaj   +1 more source

Sarcopenia and coexistent risk factors detected using the ‘Yubi-wakka’ (finger-ring) test in adults aged over 65 years in the public annual health check-up in Tama City, Tokyo: a cross-sectional study

BMJ Open, 2022
Objectives To examine the positive rate of sarcopenia using the ‘Yubi-wakka’ (finger-ring) test and associated risk factors among adults aged 65 years and older.Design Cross-sectional study.Setting We used the Yubi-wakka test, which has been developed ...
Hiroyuki Nakamura, Hitomi Fujii, Eitaro Kodani, Tomohiro Kaneko, Hajime Sasabe, Yutaka Tamura   +5 more
doaj   +1 more source

Adeno-associated virus 2 infection in children with non-A-E hepatitis

medRxiv, 2022
An outbreak of acute hepatitis of unknown aetiology in children was first reported in Scotland in April 2022. Cases aged
A. Ho, R. Orton, Rachel Tayler, P. Asamaphan, Lily Tong, Katherine Smollett, Chris Davis, Maria Manali, Sarah E. McDonald, L. Pollock, Clair, Evans, J. McMenamin, K. Roy, K. Marsh, T. Divala, Matthew T. G. Holden, M. Lockhart, D. Yirrell, S. Currie, S. Shepherd, C. Jackson, R. Gunson, A. Maclean, N. McInnes, R. Battle, J. Hollenbach, Paul, Henderson, M. Chand, M. S. Hamilton, D. Estrada-Rivadeneyra, Michael, Levin, David L. Robertson, Ana, Filipe, B. Willett, Judith Breuer, M. Semple, D. Turner, J. Kenneth, Baillie, E. Thomson   +43 more
semanticscholar   +1 more source