Results 81 to 90 of about 681,334 (361)

Mapping Hsp104 interactions using cross‐linking mass spectrometry

FEBS Open Bio, Volume 15, Issue 6, Page 922-939, June 2025.
This study examines how cross‐linking mass spectrometry can be utilized to analyze ATP‐induced conformational changes in Hsp104 and its interactions with substrates. We developed an analytical pipeline to distinguish between intra‐ and inter‐subunit contacts within the hexameric homo‐oligomer and discovered contacts between Hsp104 and a selected ...
Kinga Westphal, Karolina Michalska, Andrzej Joachimiak, Lukasz A. Joachimiak   +3 more
wiley   +1 more source

Safety of guidewire-based measurement of fractional flow reserve and the index of microvascular resistance using intravenous adenosine in patients with acute or recent myocardial infarction [PDF]

, 2016
Aims: Coronary guidewire-based diagnostic assessments with hyperemia may cause iatrogenic complications. We assessed the safety of guidewire-based measurement of coronary physiology, using intravenous adenosine, in patients with an acute coronary ...
Ahmed, Nadeem, Berry, Colin, Carberry, Jaclyn, Carrick, David, Curzen, Nick, Davie, Andrew, Eteiba, Hany, Hood, Stuart, Layland, Jamie, Lindsay, Mitchell, Mahrous, Ahmed, McConnachie, Alex, McEntegart, Margaret, Oldroyd, Keith G., Petrie, Mark C., Teng, Vannesa, Watkins, Stuart   +16 more
core   +1 more source

Purinergic Signaling and Related Biomarkers in Depression

Brain Sciences, 2020
It is established that purinergic signaling can shape a wide range of physiological functions, including neurotransmission and neuromodulation. The purinergic system may play a role in the pathophysiology of mood disorders, influencing neurotransmitter ...
Francesco Bartoli, Geoffrey Burnstock, Cristina Crocamo, Giuseppe Carrà   +3 more
doaj   +1 more source

The NAD(P)H:flavin oxidoreductase from Escherichia coli. Evidence for a new mode of binding for reduced pyridine nucleotides [PDF]

The journal of biological chemistry, American Society for Biochemistry and Molecular Biology, 1999, 274, pp.18252-60, 2015
The NAD(P)H:flavin oxidoreductase from Escherichia coli, named Fre, is a monomer of 26.2 kDa that catalyzes the reduction of free flavins using NADPH or NADH as electron donor. The enzyme does not contain any prosthetic group but accommodates both the reduced pyridine nucleotide and the flavin in a ternary complex prior to oxidoreduction.
arxiv   +1 more source

An enzyme‐linked immunosorbent assay (ELISA)‐based activity assay for AMP‐activated protein kinase (AMPK)

FEBS Open Bio, Volume 15, Issue 6, Page 914-921, June 2025.
Measuring AMP‐activated protein kinase (AMPK) activity in vitro is crucial for testing AMPK activators or inhibitors with therapeutic potential. Here, we report an enzyme‐linked immunosorbent assay (ELISA)‐based AMPK activity assay with simple steps and high sensitivity, which offers a simple, robust, and cost‐effective alternative to traditional ...
Trezze P. Nguyen, Shangze Lyu, Yang Liu
wiley   +1 more source

Targeting the small airways with dry powder adenosine: a challenging concept

European Clinical Respiratory Journal, 2017
Background: Small-particle inhaled corticosteroids (ICS) provide a higher small airway deposition than large-particle ICS. However, we are still not able to identify asthma patients who will profit most from small-particle treatment.
Erica van der Wiel, Anne J. Lexmond, Maarten van den Berge, Dirkje S. Postma, Paul Hagedoorn, Henderik W. Frijlink, Martijn P. Farenhorst, Anne H. de Boer, Nick H. T. ten Hacken   +8 more
doaj   +1 more source

Adenosine A3 receptor antagonists as anti‐tumor treatment in human prostate cancer: an in vitro study

FEBS Open Bio, EarlyView.
The A3 adenosine receptors (A3ARs) are overexpressed in prostate cancer. AR 292 and AR 357, as A3AR antagonists, are capable of blocking proliferation, modulating the expression of drug transporter genes involved in chemoresistance, ferroptosis, and the hypoxia response, and inducing cell death.
Maria Beatrice Morelli, Andrea Spinaci, Cui Chang, Rosaria Volpini, Catia Lambertucci, Matteo Landriscina, Vincenza Conteduca, Consuelo Amantini, Cristina Aguzzi, Laura Zeppa, Martina Giangrossi, Laura Soverchia, Matteo Santoni, Massimo Nabissi, Giorgio Santoni, Carlo Polidori   +15 more
wiley   +1 more source

Resetting intrinsic purinergic modulation of neural activity: an associative mechanism? [PDF]

, 2002
The purines, ATP and adenosine, control the rundown and termination of swimming in the Xenopus embryo. This intrinsic purinergic modulation, unavoidably present during every swimming episode, could lead to stereotyped inflexible behavior and consequently
Dale, Nicholas
core   +1 more source

Evaluation of Plasma Adenosine as a Marker of Cardiovascular Risk: Analytical and Biological Considerations

Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 2019
Background Adenosine is a ubiquitous regulatory molecule known to modulate signaling in many cells and processes vital to vascular homeostasis. While studies of adenosine receptors have dominated research in the field, quantification of adenosine ...
Trevor Simard, Richard Jung, Alisha Labinaz, Mohammad Ali Faraz, F. Daniel Ramirez, Pietro Di Santo, Dylan Perry‐Nguyen, Ian Pitcher, Pouya Motazedian, Chantal Gaudet, Rebecca Rochman, Jeffrey Marbach, Paul Boland, Kiran Sarathy, Saleh Alghofaili, Juan J. Russo, Etienne Couture, Steven Promislow, Rob S. Beanlands, Benjamin Hibbert   +19 more
doaj   +1 more source

Interpolated inverse discrete wavelet transforms in additive and non-additive spectral background correction [PDF]

arXiv, 2023
We demonstrate the applicability of using interpolated inverse discrete wavelet transforms as a general tool for modeling additive or multiplicative background or error signals in spectra. Additionally, we propose an unsupervised way of estimating the optimal wavelet basis along with the model parameters.
arxiv