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HELIN Acquisitions Affinity Group minutes for 3/23/12
, 2012 Minutes of the Acquisitions Affinity Group of the HELIN Consortium for March ...
HELIN Consortium. Acquisitions Affinity Group
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Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
HELIN Consortium. Digital Collections Affinity Group
, 2011 Minutes of the Digital Collections Affinity Group of the HELIN Consortium forApril ...
HELIN Digital Collections Affinity Group minutes for
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FEBS Open Bio, EarlyView.Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley +1 more source
HELIN Acquisitions Affinity Group minutes for 10/28/11
, 2011 Minutes of the Acquisitions Affinity Group of the HELIN Consortium for October ...
HELIN Consortium. Acquisitions Affinity Group
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Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Flesh, Freundschaft, and Fellowship: Towards a Holistic Model of the Amish Kinship System
The Journal of Amish and Plain Anabaptist Studies, 2020 Kinship as a social anthropological category, with its three fundamentals – affinity, descent, and siblingship – denotes an orderly system of social relationships past, present, and future, through which a social system is composed and reproduced.
Vlatka Škender
doaj
HELIN Cataloging Affinity Group minutes for 09/25/12
, 2012 Minutes of the Cataloging Affinity Group of the HELIN Consortium for September ...
HELIN Consortium. Cataloging Affinity Group
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Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
FEBS Open Bio, EarlyView.We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source
HELIN Cataloging Affinity Group minutes for 1/31/12
, 2012 Minutes of the Cataloging Affinity Group of the HELIN Consortium for January ...
HELIN Consortium. Cataloging Affinity Group
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