Results 151 to 160 of about 1,578,929 (319)
FEBS Open Bio, EarlyView.Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
HELIN E-Resources Affinity Group minutes for 2/24/11
, 2010 Minutes of the E-Resources Affinity Group of the HELIN Consortium for February ...
HELIN Consortium. E-Resources Affinity Group
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In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
FEBS Open Bio, EarlyView.Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
HELIN Access Services Affinity Group minutes for 11/17/10
, 2010 Minutes of the Access Services Affinity Group of the HELIN Consortium for November ...
HELIN Consortium. Access Services Affinity Group
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Adenosine triphosphate as a modulator of protein interactions and stability
FEBS Open Bio, EarlyView.ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley +1 more source
HELIN E-Resources Affinity Group minutes for 5/12/11
, 2011 Minutes of the E-Resources Affinity Group of the HELIN Consortium for May ...
HELIN Consortium. E-Resources Affinity Group
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FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
HELIN Digital Collections Affinity Group minutes for 11/06/14
, 2014 Minutes of the Digital Collections Affinity Group of the HELIN Consortium for November ...
HELIN Consortium. Digital Collections Affinity Group
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Heterotropic regulation and negative homotropic cooperativity
FEBS Open Bio, EarlyView.We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
HELIN Virtual User Affinity Group minutes for 6/7/12
, 2012 Minutes of the Virtual User Affinity Group of the HELIN Consortium for June ...
HELIN Consortium. Virtual User Affinity Group
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