Results 91 to 100 of about 1,074,495 (313)
, 2023 Affinity capture of an analyte by a capture agent is one of the most effective sample preparation approaches for protein analytes. We describe a new affinity capture technique for top-down protein analysis, called microprobe-capture in-emitter elution ...
Samuel, Yang, Ruben Yiqi, Luo
core +1 more source
FRACTAL ANALYSIS OF MONTHLY EVAPORATION AND PRECIPITATION TIME SERIES AT CENTRAL MEXICO
Terra Latinoamericana, 2015 Advances on climate change research, as well as the assessment of the potential impacts of climate change on water resources, would allow the understanding of the spatial and temporal variability of land-surface precipitation and evaporation time series ...
Rafael Magallanes Quintanar +5 more
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COMP–PMEPA1 axis promotes epithelial‐to‐mesenchymal transition in breast cancer cells
Molecular Oncology, EarlyView.This study reveals that cartilage oligomeric matrix protein (COMP) promotes epithelial‐to‐mesenchymal transition (EMT) in breast cancer. We identify PMEPA1 (protein TMEPAI) as a novel COMP‐binding partner that mediates EMT via binding to the TSP domains of COMP, establishing the COMP–PMEPA1 axis as a key EMT driver in breast cancer.
Konstantinos S. Papadakos +6 more
wiley +1 more source
Plasmid DNA purification via the use of a dual affinity protein
, 2008 Methods are presented for the production, affinity purification and analysis of plasmid DNA (pDNA). Batch fermentation is used for the production of the pDNA, and expanded bed chromatography, via the use of a dual affinity glutathione S-transferase (GST)
Gareth M. Forde, Forde, G.M.
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Molecular Oncology, EarlyView.Glioma cells mainly express the endothelin receptor EDNRB, while EDNRA is restricted to a perivascular tumor subpopulation. Endothelin signaling reduces glioma cell proliferation while promoting migration and a proneural‐to‐mesenchymal transition associated with poor prognosis. This pathway activates Ca2+, K+, ERK, and STAT3 signalings and is regulated
Donovan Pineau +36 more
wiley +1 more source
Molecular Oncology, EarlyView.EXOSC10, an essential nuclear RNA exosome‐associated 3′‐5′ exoribonuclease, is inhibited by the anticancer drug 5‐fluorouracil (5‐FU), and EXOSC10 depletion increases 5‐FU sensitivity. The colon‐cancer variant EXOSC10S402T, located in a proteolysis motif, is stable and nuclear but nonfunctional in vivo.
Radhika Sain +10 more
wiley +1 more source
Making sense of data streams: Complex Event Processing for Controls Applications [PDF]
, 2014 Project Specification CERN is currently investigating the usage of data analysis technologies to study the behaviour of the industrial control systems.
Voitier, Axel +2 more
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Interpreting the effects of DNA polymerase variants at the structural level
Molecular Oncology, EarlyView.Using MAVISp and molecular dynamics simulations, we analyzed over 60 000 missense variants in POLE and POLD1 from ClinVar, COSMIC, cBioPortal, and saturation mutagenesis. Identified mechanistic indicators, including stability, binding, and long‐range, enable structural interpretation, providing ACMG‐like evidence for possible reclassification of VUS ...
Matteo Arnaudi +7 more
wiley +1 more source
, 2016 The interaction between the Homer EVH domain and RyR plays an important role in calcium signaling channels in the heart and the skeletal muscles. A biosensor based on imaging ellipsometry was used to analyze the binding affinity between the Homer EVH ...
Jin, G (reprint author), Chinese Acad Sci, Beijing Key Lab Engn Construct & Mechanobiol, Inst Mech, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China. +7 more
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Molecular Oncology, EarlyView.The novel styrylquinazolinone‐based molecule W1B effectively suppresses glioblastoma by inhibiting IGF1R and EGFR. In high‐glucose microenvironments driving tumor resistance, W1B acts synergistically with the EGFR inhibitor dacomitinib. This combination safely blocks compensatory survival signaling in zebrafish xenograft models. Showcasing promising in
Patryk Rurka +9 more
wiley +1 more source