Results 81 to 90 of about 1,074,495 (313)

Affinity Analysis of Single Clones.

, 2013
Flow cytometry affinity analysis of the highest affinity clones for MMS and the best MACS/FACS clone. The cells were analyzed for affinity using 500, 250, 50, 25, 5, 2.5, 0.5, and 0 nM of the PA-Dylight 488 sample. The dissociation constant (KD) is shown
Marek Turewicz (338759), Dimitra N. Stratis-Cullum (338757), Yanting Zhang (338754), David Chang-Yen (338758), Andre de Fusco (338761), Stephen J. Moore (338755), H. Tom Soh (196866), Nancy E. Stagliano (338762), Joshua M. Kogot (338753), Paul Pagano (338756), Paul M. Pellegrino (338760)   +10 more
core   +1 more source

Energy and reactivity profile and proton affinity analysis of rimegepant with special reference to its potential activity against SARS-CoV-2 virus proteins using molecular dynamics. [PDF]

J Mol Model, 2021
Pooventhiran T, Marondedze EF, Govender PP, Bhattacharyya U, Rao DJ, Aazam ES, Kuthanapillil JM, E TJ, Thomas R.   +8 more
europepmc   +2 more sources

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

FEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

Molecular insights into the pH-dependent adsorption and removal of ionizable antibiotic oxytetracycline by adsorbent cyclodextrin polymers. [PDF]

PLoS ONE, 2014
Effects of pH on adsorption and removal efficiency of ionizable organic compounds (IOCs) by environmental adsorbents are an area of debate, because of its dual mediation towards adsorbents and adsorbate.
Yu Zhang, Xiyun Cai, Weina Xiong, Hao Jiang, Haitong Zhao, Xianhai Yang, Chao Li, Zhiqiang Fu, Jingwen Chen   +8 more
doaj   +1 more source

Phosphoinositides and inositol phosphates as molecular glues

FEBS Letters, EarlyView.
Inositol phosphates (IPs) and phosphoinositides (PIPs) regulate diverse eukaryotic processes. Beyond recruiting signaling proteins or acting as structural cofactors, recent studies suggest they mediate protein–protein interactions as natural molecular glues.
Aleshia Seaton‐Terry, Alexander J. Cutright, Mong Na Claire Loi, Jessica Martin, Joseph W. Gilligan, Jakub N. Kubina, Michael J. Arambula, Susanna Tanada Palmu, Daniella Zhou, Raymond D. Blind   +9 more
wiley   +1 more source

Affinity chromatography with immobilized DNA stationary phase for biological fingerprinting analysis of traditional Chinese medicines

, 2007
A stationary phase for high performance affinity chromatography with immobilization of DNA onto silica gel was prepared and characterized. The effect of the ionic strength, concentration of Mg2+, EDTA and CH3 CN in the mobile phase on the retention of ...
Hu, Lianghai, Zou, Hanfa, 邹汉法, Kong, Liang, Su, Xingye, Lei, Xiaoyuan   +5 more
core   +1 more source

Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis

FEBS Letters, EarlyView.
Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee, Junho Jeong, Chae Yeong Lee, Hyunjoon Cho, Man Hwan Oh, Je Chul Lee, Jee‐Young Lee, Yangmee Kim   +7 more
wiley   +1 more source

A comprehensive structural, biochemical and biological profiling of the human NUDIX hydrolase family

Nature Communications, 2017
The NUDIX hydrolases are known to be involved in several cellular processes and diseases, such as cancer, but remain poorly characterized as a family.
Jordi Carreras-Puigvert, Marinka Zitnik, Ann-Sofie Jemth, Megan Carter, Judith E. Unterlass, Björn Hallström, Olga Loseva, Zhir Karem, José Manuel Calderón-Montaño, Cecilia Lindskog, Per-Henrik Edqvist, Damian J. Matuszewski, Hammou Ait Blal, Ronnie P. A. Berntsson, Maria Häggblad, Ulf Martens, Matthew Studham, Bo Lundgren, Carolina Wählby, Erik L. L. Sonnhammer, Emma Lundberg, Pål Stenmark, Blaz Zupan, Thomas Helleday   +23 more
doaj   +1 more source

TRAIL‐PEG‐Apt‐PLGA nanosystem as an aptamer‐targeted drug delivery system potential for triple‐negative breast cancer therapy using in vivo mouse model

Molecular Oncology, EarlyView.
Aptamers are used both therapeutically and as targeting agents in cancer treatment. We developed an aptamer‐targeted PLGA–TRAIL nanosystem that exhibited superior therapeutic efficacy in NOD/SCID breast cancer models. This nanosystem represents a novel biotechnological drug candidate for suppressing resistance development in breast cancer.
Gulen Melike Demirbolat, Aslihan Kucuk, Omer Erdogan, Samed Ozer, Bensu Kozan, Tugba Cuceli, Erkan Gumus, Evrim Cevik, Ozge Cevik   +8 more
wiley   +1 more source

Expressing the human proteome for affinity proteomics: optimising expression of soluble protein domains and in vivo biotinylation.

, 2012
The generation of affinity reagents to large numbers of human proteins depends on the ability to express the target proteins as high-quality antigens. The Structural Genomics Consortium (SGC) focuses on the production and structure determination of human
Savitsky, P, Daga, N, Allerston, Charles K., Gileadi, Opher, Mahajan, Pravin, Susanne Gräslund, Gileadi, O, Hammarström, M, Müller, S, Charles K. Allerston, Opher Gileadi, Hammarström, Martin, Müller, Susanne, Phillips, Claire, Nicola A. Burgess-Brown, Cooper, CD, Keates, Tracy, Pravin Mahajan, Allerston, CK, Claire Phillips, Burgess-Brown, Nicola, Christopher D.O. Cooper, Gräslund, S, Neha Daga, Martin Hammarström, Georgina Berridge, Gräslund, Susanne, Berridge, G, Susanne Müller, Pavel Savitsky, Burgess-Brown, NA, Savitsky, Pavel, Keates, T, Mahajan, P, Tracy Keates, Daga, Neha, Berridge, Georgina, Burgess-Brown, Nicola A., Cooper, CDO, Cooper, Christopher D.O., Phillips, C   +40 more
core   +1 more source