Results 111 to 120 of about 1,074,495 (313)

Real-Time Probing of Molecular Affinity Using Optical Tweezers

Sensors
The ability to assess molecular binding kinetics in real time is critical for advancing our understanding of molecular interactions in biochemical and biotechnological systems.
Joana Teixeira, José A. Ribeiro, Marcus Monteiro, Nuno A. Silva, Pedro A. S. Jorge   +4 more
doaj   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Replication data for: Structural Equivalence and International Conflict: A Social Networks Analysis

, 2006
The concept of international affinity—albeit under different names—captures a central place in international relations research. This study examines how different types of affinity affect the likelihood of conflict between states.
Lesley G. Terris, Ilan Talmud, Ranan D. Kuperman, Zeev Maoz   +3 more
core   +1 more source

Recent Trends in Plant Protein Complex Analysis in a Developmental Context

Frontiers in Plant Science, 2018
Because virtually all proteins interact with other proteins, studying protein–protein interactions (PPIs) is fundamental in understanding protein function.
Michiel Bontinck, Michiel Bontinck, Jelle Van Leene, Jelle Van Leene, Astrid Gadeyne, Astrid Gadeyne, Bert De Rybel, Bert De Rybel, Dominique Eeckhout, Dominique Eeckhout, Hilde Nelissen, Hilde Nelissen, Geert De Jaeger, Geert De Jaeger   +13 more
doaj   +1 more source

Identification of functional murine mitochondrial formyl peptides and their effects on myeloid‐derived suppressor cell generation

FEBS Open Bio, EarlyView.
We first identified functional murine mitochondrial N‐formyl peptides (MT‐FPs) and investigated their effects on the in vitro myeloid‐derived suppressor cell (MDSC) generation from bone marrow cells. We demonstrated that MT‐FPs acted directly on bone marrow cells to promote MDSC generation and modulated the polymorphonuclear (PMN)‐MDSC/monocyte (M ...
Miyako Ozawa, Saori Kagoshima, Akira Yamada   +2 more
wiley   +1 more source

Exploring the Impact of Affinity and Unpleasantness on Conditioned Pain Modulation among Healthy Individuals

Biomedicines
The variability of the Conditioned Pain Modulation (CPM) effect can be attributed to conditioning stimulus (CS) characteristics, such as intensity, duration, unpleasantness, or affinity.
María del Rocío Ibancos-Losada, Ángeles Díaz-Fernández, Irene Cortés-Pérez, Esteban Obrero-Gaitán, Virginia López-Moreno, María Catalina Osuna-Pérez   +5 more
doaj   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Structure–Affinity Relationship Analysis and Affinity Maturation of a Calprotectin‐Binding Peptide

ChemBioChem
Peptide 3 is an 18‐amino acid linear peptide binding with sub‐micromolar affinity to the inflammation marker calprotectin. Its application in point‐of‐care diagnostic assays has shown promising results, yet improving its affinity for calprotectin could facilitate the development of sensitive and robust assays.
Lluc Farrera‐Soler, Che‐Wei Hu, Benjamin Ricken, Cristina Díaz‐Perlas, Christian‐Benedikt Gerhold, Christian Heinis   +5 more
openaire   +2 more sources

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source