Results 121 to 130 of about 1,074,495 (313)

Affinity analysis by saturation site binding assay and specificity analysis.

, 2019
Affinity analysis by saturation site binding assay and specificity analysis.
Meng Zhao (54299), Kuancan Liu (3834220), Huiling Li (260350), Xiaopeng Lan (3834226), Weibin Li (331743)   +4 more
core   +1 more source

Search Strategy Optimization of Affine Propagation Clustering Algorithm

Journal of Harbin University of Science and Technology, 2018
For multi floor fingerprint location, large scale fingerprint samples increase the complexity of the matching algorithm, which not only hinders the realtime performance of the system, but also increases the energy loss of the mobile terminal.According ...
DONG Jing-wei, ZHANG Tian-qi, LIU Yang, YANG Guang   +3 more
doaj   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Characterization of Aptamer-Protein Complexes by X-ray Crystallography and Alternative Approaches [PDF]

, 2012
Aptamers are oligonucleotide ligands, either RNA or ssDNA, selected for high-affinity binding to molecular targets, such as small organic molecules, proteins or whole microorganisms.
Levisson, M., Smidt, H., Ruigrok, V.J.B., Mark Levisson, Dijkstra, B.W., Hekelaar, J., Dijkstra, Bauke W., Hauke Smidt, Ruigrok, Vincent J.B.,, van der Oost, John, Bauke W. Dijkstra, Smidt, Hauke,, Hekelaar, Johan, Dijkstra, Bauke W.,, Hekelaar, Johan,, Vincent J. B. Ruigrok, Levisson, Mark,, Oost, J., van der, Levisson, Mark, Smidt, Hauke, Johan Hekelaar, John Van der Oost, van der Oost, J., Oost, John van der,, Ruigrok, Vincent J. B.   +24 more
core   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Studies of suppression using monoclonal regulatory T cells and the importance of co-receptor Lck coupling ratios for negative selection [PDF]

, 2015
Normal physiology is not given without immunological tolerance. Depending on the origin of induction, tolerance can be divided into central and peripheral tolerance.
Gubser, Céline
core   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

LacO-LacI interaction in affinity adsorption of plasmid DNA

, 2006
Current approaches for purifying plasmids from bacterial production systems exploit the physiochemical properties of nucleic acids in non-specific capture systems.
Ghose, Siddhartha, Slater, Nigel K.H., Darby, Richard A.J., Forde, Gareth M., Hitchcock, Anthony G., Hine, Anna V.   +5 more
core   +1 more source

Affinity Coefficient for Clustering Autoregressive Moving Average Models

Computational and Mathematical Methods
In various fields, such as economics, finance, bioinformatics, geology, and medicine, namely, in the cases of electroencephalogram, electrocardiogram, and biotechnology, cluster analysis of time series is necessary. The first step in cluster applications
Ana Paula Nascimento, Alexandra Oliveira, Brígida Mónica Faria, Rui Pimenta, Mónica Vieira, Cristina Prudêncio, Helena Bacelar-Nicolau   +6 more
doaj   +1 more source

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source