Results 11 to 20 of about 301,127 (155)

Glycoform-resolved FcɣRIIIa affinity chromatography–mass spectrometry

mAbs, 2019
Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering.
Steffen Lippold, Simone Nicolardi, Elena Domínguez-Vega, Anna-Katharina Heidenreich, Gestur Vidarsson, Dietmar Reusch, Markus Haberger, Manfred Wuhrer, David Falck   +8 more
doaj   +3 more sources

Dimerization capacities of FGF2 purified with or without heparin-affinity chromatography. [PDF]

PLoS ONE, 2014
Fibroblast growth factor-2 (FGF2) is a pleiotropic growth factor exhibiting a variety of biological activities. In this article, we studied the capacity of FGF2 purified with or without heparin affinity chromatography to self-associate. Analyzing the NMR
Natalia Platonova, Géraldine Miquel, Liang-Yuan Chiu, Said Taouji, Elisabetta Moroni, Giorgio Colombo, Eric Chevet, Shih-Che Sue, Andreas Bikfalvi   +8 more
doaj   +1 more source

Purification of baculovirus vectors using heparin affinity chromatography

Molecular Therapy: Methods & Clinical Development, 2016
Baculoviruses are commonly used for recombinant protein and vaccine production. Baculoviruses are nonpathogenic to vertebrates, have a large packaging capacity, display broad host and cell type tropism, infect both dividing and nondividing cells, and do ...
Md Nasimuzzaman, Danielle Lynn, Johannes CM van der Loo, Punam Malik   +3 more
doaj   +1 more source

Enhanced production of recombinant HALT-1 pore-forming toxin using two-step chromatographic procedure

MethodsX, 2023
Hydra actinoporin-like toxin-1 (HALT-1) has been isolated from Hydra magnipapillata and is highly cytolytic against various human cells including erythrocyte.
Wei Yuen Yap, Lok Wenn Loo, Hong Xi Sha, Jung Shan Hwang   +3 more
doaj   +1 more source

Non-targeted characterization of attributes affecting antibody-FcγRIIIa V158 (CD16a) binding via online affinity chromatography-mass spectrometry

mAbs, 2022
Antibodies facilitate targeted cell killing by engaging with immune cells such as natural killer cells through weak binding interactions with Fcγ receptors on the cell surface.
Daniel W. Woodall, Thomas M. Dillon, Kevin Kalenian, Rupa Padaki, Scott Kuhns, David J. Semin, Pavel V. Bondarenko   +6 more
doaj   +1 more source

Matrices and Affinity Ligands for Antibody Purification and Corresponding Applications in Radiotherapy

Biomolecules, 2022
Antibodies have become an important class of biological products in cancer treatments such as radiotherapy. The growing therapeutic applications have driven a demand for high-purity antibodies. Affinity chromatography with a high affinity and specificity
Aiying Xue, Saijun Fan
doaj   +1 more source

Continuous Affinity Purification of Adeno-Associated Virus Using Periodic Counter-Current Chromatography

Pharmaceutics, 2022
Replacing batch unit operations of biopharmaceuticals by continuous manufacturing is a maturing concept, with periodic counter-current chromatography (PCC) favoured to replace batch chromatography.
João P. Mendes, Magnus Bergman, Anita Solbrand, Cristina Peixoto, Manuel J. T. Carrondo, Ricardo J. S. Silva   +5 more
doaj   +1 more source

Produção, purificação e aumento da performance de ciclodextrina glicosiltransferases para produção de ciclodextrinas Production, purification and performance improve of cyclodextrin glycosyl transferases to cyclodextrins production

Química Nova, 2009
Biospecific affinity chromatography was used to purify three cyclodextrin glycosyl transferases (CGTases) obtained from microorganisms isolated of soil.
Cristiane Moriwaki, Cassiana Mazzer, Rúbia Pazzetto, Graciette Matioli   +3 more
doaj   +1 more source

Affinity chromatography: A review of trends and developments over the past 50 years. [PDF]

J Chromatogr B Analyt Technol Biomed Life Sci, 2020
Rodriguez EL, Poddar S, Iftekhar S, Suh K, Woolfork AG, Ovbude S, Pekarek A, Walters M, Lott S, Hage DS.   +9 more
europepmc   +2 more sources

Rapid determination of serum albumin partition coefficients using affinity chromatography

Environmental Advances, 2022
Sorption to serum albumin is essential for characterizing the distribution of chemicals in organisms. It impacts the concentration of the freely dissolved chemical in vivo and in vitro, and needs to be considered when effect concentrations at the target ...
Nadin Ulrich, Alexander Böhme
doaj   +1 more source