Results 21 to 30 of about 539,588 (346)

Identification of novel components of Trypanosoma brucei editosomes [PDF]

open access: yes, 2003
The editosome is a multiprotein complex that catalyzes the insertion and deletion of uridylates that occurs during RNA editing in trypanosomatids. We report the identification of nine novel editosome proteins in Trypanosoma brucei.
Carmean, Nicole   +6 more
core   +2 more sources

Purification of baculovirus vectors using heparin affinity chromatography

open access: yesMolecular Therapy: Methods & Clinical Development, 2016
Baculoviruses are commonly used for recombinant protein and vaccine production. Baculoviruses are nonpathogenic to vertebrates, have a large packaging capacity, display broad host and cell type tropism, infect both dividing and nondividing cells, and do ...
Md Nasimuzzaman   +3 more
doaj   +1 more source

Enhanced production of recombinant HALT-1 pore-forming toxin using two-step chromatographic procedure

open access: yesMethodsX, 2023
Hydra actinoporin-like toxin-1 (HALT-1) has been isolated from Hydra magnipapillata and is highly cytolytic against various human cells including erythrocyte.
Wei Yuen Yap   +3 more
doaj   +1 more source

Matrices and Affinity Ligands for Antibody Purification and Corresponding Applications in Radiotherapy

open access: yesBiomolecules, 2022
Antibodies have become an important class of biological products in cancer treatments such as radiotherapy. The growing therapeutic applications have driven a demand for high-purity antibodies. Affinity chromatography with a high affinity and specificity
Aiying Xue, Saijun Fan
doaj   +1 more source

Continuous Affinity Purification of Adeno-Associated Virus Using Periodic Counter-Current Chromatography

open access: yesPharmaceutics, 2022
Replacing batch unit operations of biopharmaceuticals by continuous manufacturing is a maturing concept, with periodic counter-current chromatography (PCC) favoured to replace batch chromatography.
João P. Mendes   +5 more
doaj   +1 more source

Sulfanilic acid-modified chitosan mini-spheres and their application for lysozyme purification from egg white [PDF]

open access: yes, 2018
A cation exchange matrix with zwitterionic and multimodal properties was synthesized by a simple reaction sequence coupling sulfanilic acid to a chitosan based support. The novel chromatographic matrix was physico-chemically characterized by ss-NMR and ζ
Baieli, María Fernanda   +7 more
core   +1 more source

Produção, purificação e aumento da performance de ciclodextrina glicosiltransferases para produção de ciclodextrinas Production, purification and performance improve of cyclodextrin glycosyl transferases to cyclodextrins production

open access: yesQuímica Nova, 2009
Biospecific affinity chromatography was used to purify three cyclodextrin glycosyl transferases (CGTases) obtained from microorganisms isolated of soil.
Cristiane Moriwaki   +3 more
doaj   +1 more source

Two-dimensional protein crystallization via metal-ion coordination by naturally occurring surface histidines [PDF]

open access: yes, 1996
A powerful and potentially general approach to the targeting and crystallization of proteins on lipid interfaces through coordination of surface histidine residues to lipid-chelated divalent metal ions is presented.
Arnold, Frances H.   +7 more
core   +1 more source

Rapid determination of serum albumin partition coefficients using affinity chromatography

open access: yesEnvironmental Advances, 2022
Sorption to serum albumin is essential for characterizing the distribution of chemicals in organisms. It impacts the concentration of the freely dissolved chemical in vivo and in vitro, and needs to be considered when effect concentrations at the target ...
Nadin Ulrich, Alexander Böhme
doaj   +1 more source

The Core Histone-binding Region of the Murine Cytomegalovirus 89K Immediate Early Protein [PDF]

open access: yes, 1991
The gene regulatory immediate early protein, pp89, of murine cytomegalovirus interacts with both DNA-associated and isolated histones in vitro. We characterized the histone-binding region of pp89 and its cellular localization during cell division to ...
Bühler, Bernhard   +3 more
core   +1 more source

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