Results 21 to 30 of about 1,855,891 (316)

Affinity Chromatography: A Historical Perspective [PDF]

Methods in molecular biology, 2015
Affinity chromatography is one of the most selective and versatile forms of liquid chromatography for the separation or analysis of chemicals in complex mixtures.
Hage, David S, Matsuda, Ryan E
core   +3 more sources

Affinity monolith chromatography: A review of general principles and recent developments

Electrophoresis, 2021
Affinity monolith chromatography (AMC) is a liquid chromatographic technique that utilizes a monolithic support with a biological ligand or related binding agent to isolate, enrich, or detect a target analyte in a complex matrix.
S. Poddar, Sadia Sharmeen, D. Hage
semanticscholar   +1 more source

Protein purification by affinity chromatography [PDF]

, 2012
Affinity chromatography is a method which depends essentially on the interaction between the molecule to be purified and a solid phase that will allow the separation of contaminants.
Coelho, L. C. B. B., Correia, M. T. S., Napoleão, Thiago H., Paiva, Patrícia M. G., Santos, Andréa F. S.   +4 more
core   +2 more sources

Affinity and Pseudo-Affinity Membrane Chromatography for Viral Vector and Vaccine Purifications: A Review

Membranes, 2023
Several chromatographic approaches have been established over the last decades for the production of pharmaceutically relevant viruses. Due to the large size of these products compared to other biopharmaceuticals, e.g., proteins, convective flow media ...
Keven Lothert, Michael W. Wolff
doaj   +1 more source

Lectin Purification through Affinity Chromatography Exploiting Macroporous Monolithic Adsorbents

Separations, 2023
Growing medical, engineering, biochemical, and biological interest has led to a steady pace of research and development into polymeric monolithic structures with densely interconnected pores for purifying bio compounds.
Josiane F. da Silva, Clara M. G. Lima, Débora L. da Silva, Ivonea S. do Nascimento, Sarah de O. Rodrigues, Letícia A. Gonçalves, Renata F. Santana, Waseem Khalid, Silvani Verruck, Talha Bin Emran, Irwin R. A. de Menezes, Henrique D. M. Coutinho, Mayeen U. Khandaker, Mohammad R. I. Faruque, Rafael da C. I. Fontan   +14 more
doaj   +1 more source

RNA-Affinity Chromatography

Bio-Protocol, 2013
RNA-affinity chromatography assays are used to identify proteins binding specific RNA sequences. These proteins represent potential factors contributing to the function of RNA molecules. In our lab, we have used this protocol to identify proteins binding
Lucia Morales , Pedro Mateos-Gomez, Luis Enjuanes, Isabel Sola   +3 more
doaj   +1 more source

Dimerization capacities of FGF2 purified with or without heparin-affinity chromatography. [PDF]

PLoS ONE, 2014
Fibroblast growth factor-2 (FGF2) is a pleiotropic growth factor exhibiting a variety of biological activities. In this article, we studied the capacity of FGF2 purified with or without heparin affinity chromatography to self-associate. Analyzing the NMR
Natalia Platonova, Géraldine Miquel, Liang-Yuan Chiu, Said Taouji, Elisabetta Moroni, Giorgio Colombo, Eric Chevet, Shih-Che Sue, Andreas Bikfalvi   +8 more
doaj   +1 more source

Affinity Chromatography of Lectins [PDF]

, 2012
Affinity chromatography is a technique used to purify compounds, such as proteins, that have the ability to non-covalently and reversibly bind specific molecules, known as ligands.
Borut Štrukelj, Janko Kos, Jure Pohleven   +2 more
core   +3 more sources

Matrices and Affinity Ligands for Antibody Purification and Corresponding Applications in Radiotherapy

Biomolecules, 2022
Antibodies have become an important class of biological products in cancer treatments such as radiotherapy. The growing therapeutic applications have driven a demand for high-purity antibodies. Affinity chromatography with a high affinity and specificity
Aiying Xue, Saijun Fan
doaj   +1 more source

Affinity Chromatography: An Enabling Technology for Large‐Scale Bioprocessing

Biotechnology Journal, 2019
Affinity chromatography (AC) has been used in large‐scale bioprocessing for almost 40 years and is considered the preferred method for primary capture in downstream processing of various types of biopharmaceuticals.
K. Łącki, F. Riske
semanticscholar   +1 more source