Results 21 to 30 of about 301,127 (155)
Single-Step Purification of Monomeric l-Selectin via Aptamer Affinity Chromatography
Sensors, 2017 l-selectin is a transmembrane receptor expressed on the surface of white blood cells and responsible for the tethering of leukocytes to vascular endothelial cells.
Christian Kuehne +2 more
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Czech Journal of Food Sciences, 2014 The properties of a previously unknown enzyme, denominated cyclodextrin glycosyltransferase, produced from Bacillus lehensis, were evaluated using affinity chromatography for protein purification.
Kate Cristina Blanco +5 more
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PVA-Glutaraldehyde as support for lectin immobilization and affinity chromatography
Acta Scientiarum: Biological Sciences, 2016 Immobilized lectins are a powerful biotechnological tool for separation and isolation of glycoconjugates. In the present study, polyvinyl alcohol (PVA) and glutaraldehyde (GA) were used as a support for Concanavalin A (Con A) covalent immobilization and ...
Moacyr Jesus Barreto de Melo Rêgo +5 more
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Purification of phage display-modified bacteriophage T4 by affinity chromatography
BMC Biotechnology, 2011 Background Affinity chromatography is one of the most efficient protein purification strategies. This technique comprises a one-step procedure with a purification level in the order of several thousand-fold, adaptable for various proteins, differentiated
Figura Grzegorz +6 more
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Purification of rabbit serum histidine-proline-rich glycoprotein via preparative gel electrophoresis and characterization of its glycosylation patterns. [PDF]
PLoS ONE, 2017 Histidine-Proline-rich Glycoprotein (HPRG) is a plasma protein of vertebrates and several marine bivalves. Due to its multidomain structure consisting of several regions HPRG can interact with a variety of ligands, however the exact physiological role ...
Anna Katharina Weyrauch +4 more
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Effect of Q Chromatography on the Recovery of Human Plasminogen in Affinity Chromatography
Zhongguo shuxue zazhi[Objective] To compare quality control (relative purity and specific activity) and process control [plasminogen (Pg) antigen recovery and potency recovery] indexes of samples before and after adding the Q chromatography step to the full chromatography ...
YUE Shenglan +12 more
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Antibody Fragments and Their Purification by Protein L Affinity Chromatography
Antibodies, 2015 Antibodies and related proteins comprise one of the largest and fastest-growing classes of protein pharmaceuticals. A majority of such molecules are monoclonal antibodies; however, many new entities are antibody fragments.
Gustav Rodrigo +2 more
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Frontiers in Endocrinology, 2015 Affinity chromatography is an efficient method to isolate proteins by taking advantage of their affinities for specific molecules such as substrates, inhibitors, antigens, ligands, antibodies, and other interacting molecules including subunits. Nowadays,
Yoko eFujita-Yamaguchi
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Single bead affinity detection (SINBAD) for the analysis of protein-protein interactions. [PDF]
PLoS ONE, 2008 We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots (QDs), are visualized on ...
Roberta Schulte +3 more
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Consecutive Affinity and Ion-Exchange Chromatography for AAV9 Vectors Purification
BiomedicinesBackground: Irrespective of the rapid development of AAV-based gene therapy, the production of clinical-grade vectors has a bottleneck resulting from product-related impurities such as empty and partially filled capsids, which lack a functional ...
Ozgun Firat Duzenli, George Aslanidi
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