Results 31 to 40 of about 1,855,891 (316)

Purification of baculovirus vectors using heparin affinity chromatography

Molecular Therapy: Methods & Clinical Development, 2016
Baculoviruses are commonly used for recombinant protein and vaccine production. Baculoviruses are nonpathogenic to vertebrates, have a large packaging capacity, display broad host and cell type tropism, infect both dividing and nondividing cells, and do ...
Md Nasimuzzaman, Danielle Lynn, Johannes CM van der Loo, Punam Malik   +3 more
doaj   +1 more source

Comparison of membrane affinity-based method with size-exclusion chromatography for isolation of exosome-like vesicles from human plasma

Journal of Translational Medicine, 2018
Plasma extracellular vesicles (EVs), especially exosome-like vesicles (ELVs), are being increasingly explored as a source of potential noninvasive disease biomarkers.
R. Stranska, Laurens Gysbrechts, Jens Wouters, P. Vermeersch, Katarzyna Bloch, D. Dierickx, G. Andrei, R. Snoeck   +7 more
semanticscholar   +1 more source

Produção, purificação e aumento da performance de ciclodextrina glicosiltransferases para produção de ciclodextrinas Production, purification and performance improve of cyclodextrin glycosyl transferases to cyclodextrins production

Química Nova, 2009
Biospecific affinity chromatography was used to purify three cyclodextrin glycosyl transferases (CGTases) obtained from microorganisms isolated of soil.
Cristiane Moriwaki, Cassiana Mazzer, Rúbia Pazzetto, Graciette Matioli   +3 more
doaj   +1 more source

Purification of rabbit serum histidine-proline-rich glycoprotein via preparative gel electrophoresis and characterization of its glycosylation patterns. [PDF]

PLoS ONE, 2017
Histidine-Proline-rich Glycoprotein (HPRG) is a plasma protein of vertebrates and several marine bivalves. Due to its multidomain structure consisting of several regions HPRG can interact with a variety of ligands, however the exact physiological role ...
Anna Katharina Weyrauch, Mario Jakob, Angelika Schierhorn, Ralf Bernd Klösgen, Dariush Hinderberger   +4 more
doaj   +1 more source

Continuous Affinity Purification of Adeno-Associated Virus Using Periodic Counter-Current Chromatography

Pharmaceutics, 2022
Replacing batch unit operations of biopharmaceuticals by continuous manufacturing is a maturing concept, with periodic counter-current chromatography (PCC) favoured to replace batch chromatography.
João P. Mendes, Magnus Bergman, Anita Solbrand, Cristina Peixoto, Manuel J. T. Carrondo, Ricardo J. S. Silva   +5 more
doaj   +1 more source

Simplifying recombinant protein production: Combining Golden Gate cloning with a standardized protein purification scheme [PDF]

arXiv, 2023
Recombinant protein production is pivotal in molecular biology, enabling profound insights into cellular processes through biophysical, biochemical, and structural analyses of the purified samples. The demand for substantial biomolecule quantities often presents challenges, particularly for eukaryotic proteins.
arxiv  

Cyclodextrin production by Bacillus lehensis isolated from cassava starch: Characterisation of a novel enzyme

Czech Journal of Food Sciences, 2014
The properties of a previously unknown enzyme, denominated cyclodextrin glycosyltransferase, produced from Bacillus lehensis, were evaluated using affinity chromatography for protein purification.
Kate Cristina Blanco, Flávio Faria De Moraes, Natalia Sozza Bernardi, Mary Helen Palmuti Braga Vettori, Rubens Monti, Jonas Contiero   +5 more
doaj   +1 more source

Single-Step Purification of Monomeric l-Selectin via Aptamer Affinity Chromatography

Sensors, 2017
l-selectin is a transmembrane receptor expressed on the surface of white blood cells and responsible for the tethering of leukocytes to vascular endothelial cells.
Christian Kuehne, Stefanie Wedepohl, Jens Dernedde   +2 more
doaj   +1 more source

Expression, Purification and Characterization of Functional Teduglutide Using GST Fusion System in Prokaryotic Cells [PDF]

Advanced Pharmaceutical Bulletin, 2023
Purpose: Teduglutide is the first and only FDA-approved drug for long-term treatment of short bowel syndrome (SBS). The current study aimed to present an approach for production of teduglutide using recombinant DNA technology.
Ali Akbar Alizadeh, Saba Rasouli, Omid Jamshidi Kandjani, Salar Hemmati, Siavoush Dastmalchi   +4 more
doaj   +1 more source

Analysis of Biological Interactions by Affinity Chromatography: Clinical and Pharmaceutical Applications.

Clinical Chemistry, 2017
BACKGROUND The interactions between biochemical and chemical agents in the body are important in many clinical processes. Affinity chromatography and high-performance affinity chromatography (HPAC), in which a column contains an immobilized biologically ...
D. Hage
semanticscholar   +1 more source