Single-Step Purification of Monomeric l-Selectin via Aptamer Affinity Chromatography
Sensors, 2017 l-selectin is a transmembrane receptor expressed on the surface of white blood cells and responsible for the tethering of leukocytes to vascular endothelial cells.
Christian Kuehne +2 more
doaj +1 more source
Purification of rabbit serum histidine-proline-rich glycoprotein via preparative gel electrophoresis and characterization of its glycosylation patterns. [PDF]
PLoS ONE, 2017 Histidine-Proline-rich Glycoprotein (HPRG) is a plasma protein of vertebrates and several marine bivalves. Due to its multidomain structure consisting of several regions HPRG can interact with a variety of ligands, however the exact physiological role ...
Anna Katharina Weyrauch +4 more
doaj +1 more source
A high-affinity antibody against the CSP N-terminal domain lacks Plasmodium falciparum inhibitory activity [PDF]
, 2020 Malaria is a global health concern and research efforts are ongoing to develop a superior vaccine to RTS,S/AS01. To guide immunogen design, we seek a comprehensive understanding of the protective humoral response against Plasmodium falciparum ...
Bosch, A. +15 more
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Czech Journal of Food Sciences, 2014 The properties of a previously unknown enzyme, denominated cyclodextrin glycosyltransferase, produced from Bacillus lehensis, were evaluated using affinity chromatography for protein purification.
Kate Cristina Blanco +5 more
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Endogenous mammalian lectin localized extracellularly in lung elastic fibers. [PDF]
, 1984 An affinity-purified antibody preparation raised against a beta-galactoside-binding lectin from bovine lung was used to localize a similar lectin in rat lung by immunofluorescence and by electron microscopy after on-grid staining visualized with ...
Barondes, SH +2 more
core +3 more sources
PVA-Glutaraldehyde as support for lectin immobilization and affinity chromatography
Acta Scientiarum: Biological Sciences, 2016 Immobilized lectins are a powerful biotechnological tool for separation and isolation of glycoconjugates. In the present study, polyvinyl alcohol (PVA) and glutaraldehyde (GA) were used as a support for Concanavalin A (Con A) covalent immobilization and ...
Moacyr Jesus Barreto de Melo Rêgo +5 more
doaj +1 more source
Activity and Process Stability of Purified Green Pepper (Capsicum annuum) Pectin Methylesterase [PDF]
, 2004 Pectin methylesterase (PME) from green bell peppers (Capsicum annuum) was extracted and purified by affinity chromatography on a CNBr-Sepharose-PMEI column. A single protein peak with pectin methylesterase activity was observed.
Alonso J. +34 more
core +2 more sources
Purification of phage display-modified bacteriophage T4 by affinity chromatography
BMC Biotechnology, 2011 Background Affinity chromatography is one of the most efficient protein purification strategies. This technique comprises a one-step procedure with a purification level in the order of several thousand-fold, adaptable for various proteins, differentiated
Figura Grzegorz +6 more
doaj +1 more source
Effect of Q Chromatography on the Recovery of Human Plasminogen in Affinity Chromatography
Zhongguo shuxue zazhi[Objective] To compare quality control (relative purity and specific activity) and process control [plasminogen (Pg) antigen recovery and potency recovery] indexes of samples before and after adding the Q chromatography step to the full chromatography ...
YUE Shenglan +12 more
doaj +1 more source
Cyclic nucleotide specificity of the activator and catalytic sites of a cGMP-stimulated cGMP phosphodiesterase from Dictyostelium discoideum [PDF]
, 1985 The cellular slime mold Dictyostelium discoideum has an intracellular phosphodiesterase which specifically hydrolyzes cGMP. The enzyme is activated by low cGMP concentrations, and is involved in the reduction of chemoattractant-mediated elevations of ...
Baraniak, Janina, +8 more
core +2 more sources