Results 21 to 30 of about 158,250 (254)
Germline-encoded specificities and the predictability of the B cell response.
PLoS Pathogens, 2023 Antibodies result from the competition of B cell lineages evolving under selection for improved antigen recognition, a process known as affinity maturation.
Marcos C Vieira +8 more
doaj +1 more source
eLife, 2018 Eliciting antibodies that are cross reactive with surface proteins of diverse strains of highly mutable pathogens (e.g., HIV, influenza) could be key for developing effective universal vaccines.
Victor Ovchinnikov +4 more
doaj +1 more source
EASINESS: E. coli Assisted Speedy affINity-maturation Evolution SyStem
Frontiers in Immunology, 2021 Antibodies are one of the most important groups of biomolecules for both clinical and basic research and have been developed as potential therapeutics. Affinity is the key feature for biological activity and clinical efficacy of an antibody, especially ...
Hai-nan Zhang +6 more
doaj +1 more source
Scientific Reports, 2021 Molecular evolution is an important step in the development of therapeutic antibodies. However, the current method of affinity maturation is overly costly and labor-intensive because of the repetitive mutation experiments needed to adequately explore ...
Koichiro Saka +7 more
doaj +1 more source
Affinity Maturation by Semi-rational Approaches [PDF]
, 2012 Rational engineering methods can be applied with success to optimize physicochemical characteristics of antibodies. Application of in silico analysis and prediction methods to antibody Fv regions can help to find residues affecting antibody-antigen affinity when high-resolution antibody structures or antibody-antigen complex structures are known.
Barderas, Rodrigo +3 more
openaire +3 more sources
Mechanisms behind Functional Avidity Maturation in T Cells
Clinical and Developmental Immunology, 2012 During an immune response antigen-primed B-cells increase their antigen responsiveness by affinity maturation mediated by somatic hypermutation of the genes encoding the antigen-specific B-cell receptor (BCR) and by selection of higher-affinity B cell ...
Marina Rode von Essen +2 more
doaj +1 more source
THE ALBUMIN-BINDING DOMAIN AS A SCAFFOLD FOR PROTEIN ENGINEERING
Computational and Structural Biotechnology Journal, 2013 The albumin-binding domain is a small, three-helical protein domain found in various surface proteins expressed by gram-positive bacteria. Albumin binding is important in bacterial pathogenesis and several homologous domains have been identified.
Johan Nilvebrant, Sophia Hober
doaj +1 more source
Nature Communications, 2019 Several vaccines are approved in the United States for seasonal influenza vaccination every year. Here we compare the impact of repeat influenza vaccination on hemagglutination inhibition (HI) titers, antibody binding and affinity maturation to ...
Surender Khurana +7 more
doaj +1 more source
eLife, 2020 Affinity maturation is a complex dynamical process allowing the immune system to generate antibodies capable of recognizing antigens. We introduce a model for the evolution of the distribution of affinities across the antibody population in germinal ...
Marco Molari +4 more
doaj +1 more source
High affinity humanized antibodies without making hybridomas; immunization paired with mammalian cell display and in vitro somatic hypermutation. [PDF]
PLoS ONE, 2012 A method has been developed for the rapid generation of high-affinity humanized antibodies from immunized animals without the need to make conventional hybridomas.
Audrey D McConnell +11 more
doaj +1 more source