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Principles of Affinity Selection
Cold Spring Harbor Protocols, 2023The most common application of phage-display technology is the discovery of peptides or proteins that specifically bind some molecule or other substance of interest—for example, antibodies that specifically bind an antigen. The discovery process starts with a library encompassing a very large array of proteins or peptides with a great diversity of ...
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Affinity-based clonal selection in Peyer’s patches
Current Opinion in Immunology, 2022Effective long-lasting immunity depends on the generation of protective antibodies that restrict the invasion of harmful pathogens. The germinal center (GC) is a microanatomical site at which B cells acquire random somatic mutations in their immunoglobulin genes followed by affinity-based selection. Whereas this process was extensively studied in lymph
Jingjing Liu, Ziv Shulman
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Lymphocyte memory and affinity selection
Journal of Theoretical Biology, 1995The persistence of antigen-specific immune memory appears to require the presence of antigen--suggesting that memory may be due to restimulation of "memory" lymphocytes by persisting antigen. Persistence of antigen, in a form capable of stimulating B cell proliferation, on long-lived, follicular dendritic cells of lymphoid tissue is well documented ...
M A, Fishman, A S, Perelson
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Affinity selection mass spectrometry speeding drug discovery
Drug Discovery Today, 2023Affinity selection mass spectrometry (AS-MS) has gained momentum in drug discovery. This review summarizes how this technology has slowly risen as a new paradigm in hit identification and its potential synergy with DNA encoded library technology. It presents an overview of the recent results on challenging targets and perspectives on new areas of ...
Renaud Prudent +3 more
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Driving Affinity Selection by Centrifugal Force
ASSAY and Drug Development Technologies, 2003We describe a new approach to affinity selection based on the application of centrifugal force to macromolecules in solution. The method relies on the well known macromolecular hydrodynamic principles of centrifugation. It can be automated and operated in a centralized fashion, or it can be decentralized and used by single researchers or networks of ...
J E, Harlan +6 more
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Ergoline derivatives: receptor affinity and selectivity
Il Farmaco, 1999Ergot comprises a group of indole alkaloids which are predominantly found in various species of the ascomycete Claviceps. In pharmacopoeias, the sclerotia of Claviceps purpurea (Fr.) Tulasne parasitizing on rye, Secale cereale L., are designed as ergot or Secale cornutum.
S, Mantegani, E, Brambilla, M, Varasi
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Antibody affinity maturation in selectively bred high and low‐affinity mice
European Journal of Immunology, 1986AbstractThe affinity of serum antibodies produced by selectively bred lines of mice [high affinity, low affinity, low nonmaturing (N/M)] injected with T‐dependent [human serum albumin (HSA), dinitrophenylated bovine γ‐globulin (DNP‐BGG)] and T‐independent (DNP‐Ficoll) antigens in saline and adjuvant has been determined.
M W, Steward, C, Stanley, M D, Furlong
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Affinity selection and sequencing
Nature Chemical Biology, 2019Powerful combinatorial peptide library methods allow the discovery of peptide leads from diverse libraries. A new platform based on tandem mass spectrometry peptide sequencing coupled with high-performance size-exclusion chromatography enables identification of high-affinity peptidic ligands from focused libraries.
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