Results 181 to 190 of about 50,213 (209)
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ATG-anchored AFLP (ATG-AFLP) analysis in cotton
Plant Cell Reports, 2008Amplified fragment length polymorphism (AFLP) marker system has had broad applications in biology. However, the anonymous AFLP markers are mainly amplified from non-coding regions, limiting their usefulness as a functional marker system. To take advantages of the traditional AFLP techniques, we propose substitution of a restriction enzyme that ...
Yingzhi, Lu +4 more
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AFLP genotyping and fingerprinting
Trends in Ecology & Evolution, 1999Amplified fragment length polymorphisms (AFLPs) are polymerase chain reaction (PCR)-based markers for the rapid screening of genetic diversity. AFLP methods rapidly generate hundreds of highly replicable markers from DNA of any organism; thus, they allow high-resolution genotyping of fingerprinting quality.
, Mueller, , Wolfenbarger
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AFLP-based transcript profiling (cDNA-AFLP) for genome-wide expression analysis
Nature Protocols, 2007Although DNA microarrays are currently the standard tool for genome-wide expression analysis, their application is limited to organisms for which the complete genome sequence or large collections of known transcript sequences are available. Here, we describe a protocol for cDNA-AFLP, an AFLP-based transcript profiling method that allows genome-wide ...
Marnik, Vuylsteke +2 more
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AFLP technology for DNA fingerprinting
Nature Protocols, 2007The AFLP technique is a powerful DNA fingerprinting technology applicable to any organism without the need for prior sequence knowledge. The protocol involves the selective PCR amplification of restriction fragments of a total digest of genomic DNA, typically obtained with a mix of two restriction enzymes.
Marnik, Vuylsteke +2 more
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AbstractThis unit presents an alternative to differential display that allows the quantification of transcripts, based on AFLP‐fingerprinting of double‐stranded cDNA. The protocol described includes the following steps: the isolation of poly(A)+ RNA from total RNA, the synthesis of double‐stranded cDNA, the preparation of template fragments by ...
Pieter, Vos, Patrick, Stanssens
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AFLP runner (AR): A software for prediction and analysis Of AFLP
2010 3rd International Conference on Biomedical Engineering and Informatics, 2010AFLP is a PCR-based tool used in genetics research and DNA fingerprinting. Predicting the result and selecting the right primers play key roles in AFLP. Software called AFLP Runner (AR) was developed for AFLP predicting and analyzing. AR is a software based KMP algorithm for finding restriction enzyme cutting site sequence in genome. AR can be used for
Meng Zhang +5 more
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AFLP variation in 25 Avena species
Theoretical and Applied Genetics, 2008Current molecular characterization of ex situ plant germplasm has placed more emphasis on cultivated gene pools and less on exotic gene pools representing wild relative species. This study attempted to characterize a selected set of germplasm accessions representing various Avena species with the hope to establish a reference set of exotic oat ...
Yong-Bi, Fu, David J, Williams
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Use of AFLP in cereals research
Trends in Plant Science, 1999Bands of interest can be excised from gels that have been dried onto 3MM filter paper (Whatman) or from dried silver stained gels17xCloning and mapping of variety-specific rice genomic DNA sequences: amplified fragment length polymorphisms (AFLP) from silver stained polyacrylamide gels. Cho, Y.G. et al. Genome. 1996; 39: 373–378Crossref | PubMedSee all
, Ridout, , Donini
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Development of AFLP markers in barley
Molecular and General Genetics MGG, 1997To investigate the application of amplified fragment length polymorphism (AFLP) markers in barley, 96 primer combinations were used to generate AFLP patterns with two barley lines, L94 and Vada. With seven primer combinations, only a few intense bands were obtained, probably derived from repeated sequences.
Qi, X., Lindhout, P.
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