Results 221 to 230 of about 40,833 (276)
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AFLP variation in 25 Avena species
Theoretical and Applied Genetics, 2008Current molecular characterization of ex situ plant germplasm has placed more emphasis on cultivated gene pools and less on exotic gene pools representing wild relative species. This study attempted to characterize a selected set of germplasm accessions representing various Avena species with the hope to establish a reference set of exotic oat ...
Yong-Bi, Fu, David J, Williams
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AFLP fingerprinting for paternity testing in ducks
British Poultry Science, 20071. The accuracy and reproducibility of AFLP fingerprinting was investigated in the duck (Anas Platyrhynchos), using a multicolour fluorescent labeling technique. The fluorescent labelling fragments were separated on a capillary electrophoresis-base ABI PRISM 3100 Genetic Analyzer. 2.
Huang, C.W. +5 more
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The status of AFLP in the genomics era and a pipeline for converting AFLPs into single-locus markers
Molecular Breeding, 2014Even though next-generation sequencing (NGS) has now become the predominant state-of-the-art technique for genotyping populations, amplified fragment length polymorphism (AFLP) DNA fingerprinting is still a relevant method, thanks to its versatility, cost-effectiveness, independence of prior sequence information and broad applicability. Even though the
Zhengfeng Zhang +2 more
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AFLP data and the origins of domesticated crops
Genome, 2003Amplified fragment length polymorphism (AFLP) datasets have been used to construct neighbor-joining trees from which monophyletic origins for crops such as einkorn wheat, barley, and emmer wheat have been inferred. We simulated several different multiple domestication scenarios for an imaginary cereal crop and examined the resulting domesticated ...
Allaby, Robin G., Brown, Terence A.
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AbstractThis unit presents an alternative to differential display that allows the quantification of transcripts, based on AFLP‐fingerprinting of double‐stranded cDNA. The protocol described includes the following steps: the isolation of poly(A)+ RNA from total RNA, the synthesis of double‐stranded cDNA, the preparation of template fragments by ...
Pieter, Vos, Patrick, Stanssens
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AFLP™ markers for DNA fingerprinting in cattle
Animal Genetics, 1997This work reports on use of the recently described amplified fragment length polymorphism (AFLP) technology for DNA fingerprinting in cattle. The AFLP technology produces molecular markers through the high‐stringency polymerase chain reaction (PCR)‐amplification of restriction fragments that are ligated to synthetic adapters and amplified using primers,
AJMONE MARSAN P +5 more
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Multilocus Profiling with AFLP, ISSR, and SAMPL
2013Molecular markers which sample multiple loci simultaneously, like amplified fragment length polymorphism (AFLP), inter-simple sequence repeats (ISSR), and selective amplification of microsatellite polymorphic loci (SAMPL), produce highly informative fingerprints due to their high effective multiplex ratio and expected heterozygosity.
Luis F, Goulao, Cristina M, Oliveira
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Genome, 2004
Amplified fragment length polymorphism (AFLP) has proven a powerful tool for tagging genes or quantitative trait loci (QTLs) of interest in plants. However, conversion of AFLP markers into sequence-tagged site (STS) markers is technically challenging in wheat owing to the complicated nature of its genome. In this study, we developed an "extension-AFLP"
D H, Xu, T, Ban
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Amplified fragment length polymorphism (AFLP) has proven a powerful tool for tagging genes or quantitative trait loci (QTLs) of interest in plants. However, conversion of AFLP markers into sequence-tagged site (STS) markers is technically challenging in wheat owing to the complicated nature of its genome. In this study, we developed an "extension-AFLP"
D H, Xu, T, Ban
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Crop Science, 2001
Amplified restriction fragment length polymorphic (AFLP) DNA analysis was performed on leaf samples of 40 accessions of opium poppy (Papaver somniferum L.) and two other control genera (Papaver bracteatum Lindley and Papaver setigerum DC.) from a commercial breeding collection held in Tasmania, Australia.
James A. Saunders +3 more
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Amplified restriction fragment length polymorphic (AFLP) DNA analysis was performed on leaf samples of 40 accessions of opium poppy (Papaver somniferum L.) and two other control genera (Papaver bracteatum Lindley and Papaver setigerum DC.) from a commercial breeding collection held in Tasmania, Australia.
James A. Saunders +3 more
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MICROSATELLITE AND AFLP MARKERS IN AN ARTICHOKE WORLD COLLECTION
Acta Horticulturae, 2004Artichoke, Cynara cardunculus L. var. scolymus (L.) Fiori is a diploid outcrossing species, originated in the Mediterranean basin, which has been much appreciated both for its tasting heads and pharmaceutical properties since ancient times. In order to assess genetic variation and relationships among artichoke varieties and between these and their wild
Sonnante G, Ippedico M, De Paolis A
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