Results 41 to 50 of about 1,463,134 (306)
The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation
FEBS Letters, EarlyView.Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge +6 more
wiley +1 more source
DNA ligase LigE increases bacterial transformation with damaged extracellular DNA
FEBS Letters, EarlyView.The DNA ligase LigE from the naturally competent Gram‐negative bacterium Neisseria gonorrhoaea increases transformation with cut extracellular DNA. This process is ATP‐dependent and relies on the catalytic activity of LigE; it also requires that LigE contains an intact periplasmic localisation sequence, indicating that ligE functions in the periplasm ...
Jolyn Pan +4 more
wiley +1 more source
Research on seaweeds and mollusks [PDF]
, 1995 Research on seaweeds focused on the carrageenan-producing Kappaphycus alvarezii and the agar-producing Gracilaria spp. Growth of K. alvarezii was better on horizontal lines than on vertical or cluster lines from bamboo rafts. All morphotypes (brown green,
Hurtado-Ponce, Anicia Q.
core +1 more source
AgronomyPurslane is highly suitable for intensive microgreen cultivation due to its rapid growth, high germination rate, and exceptional nutritional profile, including omega-3 fatty acids, essential vitamins, and minerals.
Ivana Kollárová +6 more
doaj +1 more source
Molecular Oncology, EarlyView.Partial inhibition of focal adhesion kinase (FAK) can paradoxically promote tumor growth, rather than simply producing a weaker antitumor effect than that observed with strong FAK suppression. In breast cancer and melanoma models, targeting p110δ PI3K, particularly in macrophages, counteracted these tumor‐promoting effects, highlighting the importance ...
Lydia Xenou +4 more
wiley +1 more source
Alkaline peptone water plus 0.5% agar suitable for transport of Vibrio cholerae
Universa Medicina, 2016 Microbiological procedures for V. cholerae isolation from clinical specimens are important factors in clinical and epidemiological management of cholera. The standard preservation medium for enteric pathogenic bacteria, including V.
Meiyanti Meiyanti +3 more
doaj +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Effect of Homogenized Callus Tissue on the Rheological and Mechanical Properties of 3D-Printed Food
GelsThe aim of the study was to develop ink enriched with a high content of lupine callus tissue (CT) suitable for 3D printing. Printable ink obtained using mashed potatoes (20 g/100 mL) and a 3% agar solution was used as the parent CT-free ink (CT0 ...
Elena Dushina +7 more
doaj +1 more source
EFFICIENT in vitro PROPAGATION OF Amaranthus viridis L. USING NODE EXPLANTS
Acta Scientiarum Polonorum: Hortorum Cultus, 2020 Hyperhydricity is a frequently problem in plants during in vitro culture and affected micropropagation of plants. To develop an efficient in vitro regenerated system without hyperdydricity, we demonstrated the effect of different disinfected agents ...
Tour Jan +6 more
doaj +1 more source