Results 31 to 40 of about 587,845 (265)

Identification of the plant mitochondrial OrfX protein: A mass spectrometry approach

FEBS Letters, EarlyView.
The mitochondrial genome of plants contains an open reading frame, orfx, which encodes a rare protein that has so far escaped mass spectrometric detection. The protein resembles the c‐subunit of bacterial twin‐arginine‐motif‐dependent protein translocases (TatC).
Matthias Döring, Jan de Jonge, Hans‐Peter Braun, Nils Rugen   +3 more
wiley   +1 more source

Structure and Function of Recombinant versus Plasma-Derived von Willebrand Factor and Impact on Multimer Pharmacokinetics in von Willebrand Disease

Journal of Blood Medicine, 2022
Herbert Gritsch,1 Gerald Schrenk,1 Nina Weinhappl,1 Björn Mellgård,2 Bruce Ewenstein,2 Peter L Turecek3,4 1Pharmaceutical Sciences, Baxalta Innovations GmbH, a Takeda Company, Vienna, Austria; 2Rare Genetics and Hematology, Research & Development, Takeda
Gritsch H, Schrenk G, Weinhappl N, Mellgård B, Ewenstein B, Turecek PL   +5 more
doaj  

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Agar-based adaptable DIY materials [PDF]

FME Transactions, 2019
Several types of agar-based polymeric materials were developed for the use of designers, engineers, makers, and do-it-yourself (DIY) enthusiasts. Simple recipes and procedures were determined for the adaptation and use by anyone who is interested to use ...
Zuberi Hajra S., Bengisu Murat
doaj  

Composição do meio de cultura e condições ambientais para germinação de grãos de pólen de porta-enxertos de pereira Medium composition and environmental conditions for the germination of pollen grains of pear rootsocks

Ciência Rural, 2010
Visando a dar suporte a trabalhos de melhoramento genético para porta-enxertos de pera e ajuste de protocolo de germinação de pólen para fins de polinização intra e interespécies, objetivou-se ajustar os componentes básicos do meio de cultura e as ...
Edvan Alves Chagas, Rafael Pio, Pollyana Cardoso Chagas, Moacir Pasqual, José Emílio Bettiol Neto   +4 more
doaj   +1 more source

Time‐restricted feeding prior to Mycobacterium tuberculosis infection reduces tissue CD4+ T cells with limited impact on bacterial clearance

FEBS Open Bio, EarlyView.
Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta, Nidhi Yadav, Subhasmita Das, R. Rajendra Kumar Reddy, Nupur Sharma, Amol Ratnakar Suryawanshi, Jaswinder Singh Maras, Ranjan Kumar Nanda   +7 more
wiley   +1 more source

Regulatory Effects of RNA–Protein Interactions Revealed by Reporter Assays of Bacteria Grown on Solid Media

Biosensors
Reporter systems are widely used to study biomolecular interactions and processes in vivo, representing one of the basic tools used to characterize synthetic regulatory circuits.
Guillermo Pérez-Ropero, Roswitha Dolcemascolo, Anna Pérez-Ràfols, Karl Andersson, U. Helena Danielson, Guillermo Rodrigo, Jos Buijs   +6 more
doaj   +1 more source

Anisotropic Conductivity of Rat Head Phantom and its Influence on Electroencephalogram Source Localization

IEEE Access, 2022
In this paper we deal with a simplified anisotropic rat head phantom development and the investigation of the influence of the anisotropic white matter on electroencephalogram source localization.
Jaroslav Lacik, Vlastimil Koudelka, Cestmir Vejmola, David Kuratko, Jiri Vanek, Daniel Krzysztof Wojcik, Tomas Palenicek, Zbynek Raida   +7 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source