Results 41 to 50 of about 19,917 (239)

Insights into Conformational Dynamics and Allostery in DNMT1-H3Ub/USP7 Interactions

Molecules, 2021
DNA methyltransferases (DNMTs) including DNMT1 are a conserved family of cytosine methylases that play crucial roles in epigenetic regulation. The versatile functions of DNMT1 rely on allosteric networks between its different interacting partners ...
Yu Zhu, Fei Ye, Ziyun Zhou, Wanlin Liu, Zhongjie Liang, Guang Hu   +5 more
doaj   +1 more source

Filamentation modulates allosteric regulation of PRPS

eLife, 2022
Phosphoribosyl pyrophosphate (PRPP) is a key intermediate in the biosynthesis of purine and pyrimidine nucleotides, histidine, tryptophan, and cofactors NAD and NADP.
Huan-Huan Hu, Guang-Ming Lu, Chia-Chun Chang, Yilan Li, Jiale Zhong, Chen-Jun Guo, Xian Zhou, Boqi Yin, Tianyi Zhang, Ji-Long Liu   +9 more
doaj   +1 more source

Insights into the Allosteric Regulation of Human Hsp90 Revealed by NMR Spectroscopy

Biomolecules
Human heat shock protein 90 (Hsp90) is one of the most important chaperones that play a role in the late stages of protein folding. Errors in the process of the chaperone cycle can lead to diseases such as cancer and neurodegenerative diseases. Therefore,
Tjaša Goričan, Simona Golič Grdadolnik   +1 more
doaj   +1 more source

Identification of Alternative Allosteric Sites in Glycolytic Enzymes for Potential Use as Species-Specific Drug Targets

Frontiers in Molecular Biosciences, 2020
Three allosteric glycolytic enzymes, phosphofructokinase, glyceraldehyde-3 phosphate dehydrogenase and pyruvate kinase, associated with bacterial, parasitic and human species, were explored to identify potential allosteric sites that would be used as ...
Merve Ayyildiz, Serkan Celiker, Fatih Ozhelvaci, E. Demet Akten   +3 more
doaj   +1 more source

FimH as a scaffold for regulated molecular recognition

Journal of Biological Engineering, 2021
Background Recognition proteins are critical in many biotechnology applications and would be even more useful if their binding could be regulated. The current gold standard for recognition molecules, antibodies, lacks convenient regulation.
Shivani Gupta Ludwig, Casey L. Kiyohara, Laura A. Carlucci, Dagmara Kisiela, Evgeni V. Sokurenko, Wendy Evelyn Thomas   +5 more
doaj   +1 more source

Ubiquitination of transcription factors in cancer: unveiling therapeutic potential

Molecular Oncology, EarlyView.
In cancer, dysregulated ubiquitination of transcription factors contributes to the uncontrolled growth and survival characteristics of tumors. Tumor suppressors are degraded by aberrant ubiquitination, or oncogenic transcription factors gain stability through ubiquitination, thereby promoting tumorigenesis.
Dongha Kim, Hye Jin Nam, Sung Hee Baek
wiley   +1 more source

Integrative residue-intuitive machine learning and MD Approach to Unveil Allosteric Site and Mechanism for β2AR

Nature Communications
Allosteric drugs offer a new avenue for modern drug design. However, the identification of cryptic allosteric sites presents a formidable challenge. Following the allostery nature of residue-driven conformation transition, we propose a state-of-the-art ...
Xin Chen, Kexin Wang, Jianfang Chen, Chao Wu, Jun Mao, Yuanpeng Song, Yijing Liu, Zhenhua Shao, Xuemei Pu   +8 more
doaj   +1 more source

Purification and characterization of a thermophilic NAD+‐dependent lactate dehydrogenase from Moorella thermoacetica

FEBS Open Bio, EarlyView.
The thermophilic acetogenic model organism Moorella thermoacetica can utilize lactate as substrate. The formation of lactate by reduction of pyruvate is energetically favorable, but the reverse reaction, the oxidation of lactate to pyruvate with NAD+ as electron acceptor is challenging.
Florian P. Rosenbaum, Volker Müller
wiley   +1 more source

The Chemerin Receptor CMKLR1 Requires Full‐Length Chemerin for High Affinity in Contrast to GPR1 as Demonstrated by a New Nanoluciferase‐Based Binding Assay

ChemMedChem, Volume 17, Issue 23, December 5, 2022., 2022
The adipokine chemerin and the short peptide agonist chemerin‐9 activate two GPCRs: CMKLR1 and GPR1. Here, we established a nanoluciferase based binding assay to analyze differences in ligand binding. For GPR1, both ligands show high affinity and comparable binding.
Anne Sophie Czerniak, Kevin Kretschmer, Tina Weiß, Annette G. Beck‐Sickinger   +3 more
wiley   +1 more source

An enzyme‐linked immunosorbent assay (ELISA)‐based activity assay for AMP‐activated protein kinase (AMPK)

FEBS Open Bio, EarlyView.
Measuring AMP‐activated protein kinase (AMPK) activity in vitro is crucial for testing AMPK activators or inhibitors with therapeutic potential. Here, we report an enzyme‐linked immunosorbent assay (ELISA)‐based AMPK activity assay with simple steps and high sensitivity, which offers a simple, robust, and cost‐effective alternative to traditional ...
Trezze P. Nguyen, Shangze Lyu, Yang Liu
wiley   +1 more source