Results 41 to 50 of about 67,162 (289)

Insights into Conformational Dynamics and Allostery in DNMT1-H3Ub/USP7 Interactions

Molecules, 2021
DNA methyltransferases (DNMTs) including DNMT1 are a conserved family of cytosine methylases that play crucial roles in epigenetic regulation. The versatile functions of DNMT1 rely on allosteric networks between its different interacting partners ...
Yu Zhu, Fei Ye, Ziyun Zhou, Wanlin Liu, Zhongjie Liang, Guang Hu   +5 more
doaj   +1 more source

Identification of Alternative Allosteric Sites in Glycolytic Enzymes for Potential Use as Species-Specific Drug Targets

Frontiers in Molecular Biosciences, 2020
Three allosteric glycolytic enzymes, phosphofructokinase, glyceraldehyde-3 phosphate dehydrogenase and pyruvate kinase, associated with bacterial, parasitic and human species, were explored to identify potential allosteric sites that would be used as ...
Merve Ayyildiz, Serkan Celiker, Fatih Ozhelvaci, E. Demet Akten   +3 more
doaj   +1 more source

FimH as a scaffold for regulated molecular recognition

Journal of Biological Engineering, 2021
Background Recognition proteins are critical in many biotechnology applications and would be even more useful if their binding could be regulated. The current gold standard for recognition molecules, antibodies, lacks convenient regulation.
Shivani Gupta Ludwig, Casey L. Kiyohara, Laura A. Carlucci, Dagmara Kisiela, Evgeni V. Sokurenko, Wendy Evelyn Thomas   +5 more
doaj   +1 more source

Integrative residue-intuitive machine learning and MD Approach to Unveil Allosteric Site and Mechanism for β2AR

Nature Communications
Allosteric drugs offer a new avenue for modern drug design. However, the identification of cryptic allosteric sites presents a formidable challenge. Following the allostery nature of residue-driven conformation transition, we propose a state-of-the-art ...
Xin Chen, Kexin Wang, Jianfang Chen, Chao Wu, Jun Mao, Yuanpeng Song, Yijing Liu, Zhenhua Shao, Xuemei Pu   +8 more
doaj   +1 more source

Protein dynamics underlying allosteric regulation

Current Opinion in Structural Biology
Allostery is the mechanism by which information and control are propagated in biomolecules. It regulates ligand binding, chemical reactions, and conformational changes. An increasing level of experimental resolution and control over allosteric mechanisms promises a deeper understanding of the molecular basis for life and powerful new therapeutics.
Miro A. Astore, Akshada S. Pradhan, Erik H. Thiede, Sonya M. Hanson   +3 more
openaire   +2 more sources

Allosteric regulation of epigenetic modifying enzymes [PDF]

Current Opinion in Chemical Biology, 2017
Epigenetic enzymes including histone modifying enzymes are key regulators of gene expression in normal and disease processes. Many drug development strategies to target histone modifying enzymes have focused on ligands that bind to enzyme active sites, but allosteric pockets offer potentially attractive opportunities for therapeutic development. Recent
Beth E, Zucconi, Philip A, Cole
openaire   +2 more sources

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source

Design of an allosterically regulated retroaldolase [PDF]

Protein Science, 2015
AbstractWe employed a minimalist approach for design of an allosterically controlled retroaldolase. Introduction of a single lysine residue into the nonenzymatic protein calmodulin led to a 15,000‐fold increase in the second order rate constant for retroaldol reaction with methodol as a substrate.
Elizabeth A, Raymond, Korrie L, Mack, Jennifer H, Yoon, Olesia V, Moroz, Yurii S, Moroz, Ivan V, Korendovych   +5 more
openaire   +2 more sources

Integration of circadian and hypoxia signaling via non‐canonical heterodimerization

FEBS Letters, EarlyView.
CLOCK, BMAL1, and HIFs are basic helix‐loop‐helix and Per‐Arnt‐Sim domain (bHLH‐PAS) proteins, which function as transcription factors. bHLH‐PAS proteins are designated in two classes. Many class I proteins are regulated by environmental signals via their PAS domains, but such signals have not been identified for all.
Sicong Wang, Katja A. Lamia
wiley   +1 more source

Allosteric regulation of substrate channeling: Salmonella typhimurium tryptophan synthase

Frontiers in Molecular Biosciences, 2022
The regulation of the synthesis of L-tryptophan (L-Trp) in enteric bacteria begins at the level of gene expression where the cellular concentration of L-Trp tightly controls expression of the five enzymes of the Trp operon responsible for the synthesis ...
Rittik K. Ghosh, Eduardo Hilario, Chia-en A. Chang, Leonard J. Mueller, Michael F. Dunn   +4 more
doaj   +1 more source