Results 151 to 160 of about 1,411 (182)

Characterization of a Recombinant d-Allulose 3-epimerase from Thermoclostridium caenicola with Potential Application in d-Allulose Production

Molecular Biotechnology, 2021
In recent years, with the increasing public health awareness, low-calorie rare sugars have received more attention on a global scale. D-Allulose, the C-3 epimer of D-fructose, is a representative rare sugar. It displays high sweetness and excellent physiological functions, but only provides a caloric value of 0.4 kcal/g. D-Allulose 3-epimerase (DAEase)
Jiajun Chen, Ding Chen, Mengyu Ke, Shengyuan Ye, Xinyu Wang, Wenli Zhang, Wanmeng Mu   +6 more
openaire   +2 more sources

Properties of d-allulose 3-epimerase mined from Novibacillus thermophilus and its application to synthesis of d-allulose

Enzyme and Microbial Technology, 2021
Abstract • Allulose is an epimer of d -fructose with multiple nutritional functions, which can be biologically synthesized from d -fructose employing d -allulose 3-epimerase (DAE). In this study, NCBI database mining approach was applied to screen novel DAEs, and Novibacillus thermophilus DAE (NtDAE) exhibiting a high activity of 273.9 U/(g ...
Dong-Xu Jia, Chen-Yi Sun, Yi-Ting Jin, Zhi-Qiang Liu, Yu-Guo Zheng, Mian Li, Hong-Yan Wang, De-Shui Chen   +7 more
openaire   +2 more sources

Directional immobilization of D-allulose 3-epimerase using SpyTag/SpyCatcher strategy as a robust biocatalyst for synthesizing D-allulose

Food Chemistry, 2023
D-Allulose, as low-calorie rare sugar, possessed several notable biological activities and was biosynthesized by D-allulose 3-epimerase (DAEase). Here, CcDAE from Clostridium cellulolyticum was successfully immobilization via covalent attachment (RI-CcDAE), and Resin-SpyCatcher/SpyTag-CcDAE modular (DI-CcDAE).
Xin, Gao, Cancan, Wei, Hongbin, Qi, Chao, Li, Fuping, Lu, Hui-Min, Qin   +5 more
openaire   +2 more sources

Efficient enzymatic synthesis of d‐allulose using a novel d‐allulose‐3‐epimerase from Caballeronia insecticola

Journal of the Science of Food and Agriculture, 2022
AbstractBACKGROUNDRare sugars have become promising ‘sugar alternatives’ because of their low calories and unique physiological functions. Among the family of rare sugars, d‐allulose is one of the sugars attracting interest. Ketose 3‐epimerases (KEase), including d‐tagatose 3‐epimerase (DTEase) and d‐allulose 3‐epimerase (DAEase), are mainly used for d‐
Zijie Li, Linxue Feng, Zhou Chen, Yangfan Hu, Kangqing Fei, Huilin Xu, Xiao‐Dong Gao   +6 more
openaire   +2 more sources

Allulose in human diet: the knowns and the unknowns

British Journal of Nutrition, 2021
AbstractD-Allulose, also referred to as psicose, is a C3-epimer of D-fructose used as a sugar substitute in low energy products. It can be formed naturally during processing of food and drinks containing sucrose and fructose or is prepared by chemical synthesis or via enzymatic treatment with epimerases from fructose.
Hannelore, Daniel, Hans, Hauner, Mathias, Hornef, Thomas, Clavel   +3 more
openaire   +2 more sources

Fusion and secretory expression of an exo‐inulinase and a d‐allulose 3‐epimerase to produce d‐allulose syrup from inulin

Journal of the Science of Food and Agriculture, 2020
AbstractBACKGROUNDThis study developed a feasible catalytic method for d‐allulose syrup production using a fusion enzyme, either in free or immobilized form, through hydrolysis of inulin extracted from Jerusalem artichoke tubers.RESULTSd‐Allulose 3‐epimerase (DAE) was actively expressed in secretory form by fusing with the extracellular exo‐inulinase ...
Yueyuan Huang, Liangfei Li, Yaowei Chi, Yuanyuan Sha, Rui Wang, Zheng Xu, Xiaoqi Xu, Sha Li, Zhen Gao, Hong Xu   +9 more
openaire   +2 more sources

D-allulose 3-epimerase for low-calorie D-allulose synthesis: microbial production, characterization, and applications

Critical Reviews in Biotechnology
D-allulose, an epimer of D-fructose at C-3 position, is a low-calorie rare sugar with favorable physiochemical properties and special physiological functions, which displays promising perspectives in the food and pharmaceutical industries. Currently, D-allulose is extremely sparse in nature and is predominantly biosynthesized through the isomerization ...
Xiaofang Xie, Caiming Li, Xiaofeng Ban, Hongshun Yang, Zhaofeng Li   +4 more
openaire   +2 more sources

d-Allulose enhances postprandial fat oxidation in healthy humans

Nutrition, 2017
d-Allulose, a C-3 epimer of d-fructose, has been reported to decrease body weight and adipose tissue weight in animal studies and is expected to be a potent antiobese sweetener. Our animal study suggested that one of the mechanisms of d-allulose's antiobesity function is an increase in energy expenditure.
Tomonori, Kimura, Akane, Kanasaki, Noriko, Hayashi, Takako, Yamada, Tetsuo, Iida, Yasuo, Nagata, Kazuhiro, Okuma   +6 more
openaire   +2 more sources

Tailored magnetic silica-immobilized D-allulose 3-epimerase with enhanced stability and recyclability for efficient D-allulose production

International Journal of Biological Macromolecules
D-allulose, a low-calorie functional sweetener, is produced by the enzymatic conversion of d-fructose via D-allulose 3-epimerase (DAE) and holds significant market potential, particularly for individuals with obesity and diabetes. However, the limited reusability and stability of DAE have restricted its industrial application.
Ziqun, Xiao, Bo, Jiang, Longbei, Xiang, Ran, Zhang, Jingjing, Chen   +4 more
openaire   +2 more sources