Results 91 to 100 of about 2,258,983 (316)

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source

Implementation of the 3Rs Concept in Quality Control of Biologicals: Status and Prospects (Review)

Безопасность и риск фармакотерапии
INTRODUCTION. Many countries are implementing an increasing number of alternatives to animal testing in the research, development, and quality control of medicines under the concept of 3Rs (replacement, reduction, and refinement).
L. A. Gaiderova, N. A. Alpatova, O. V. Golovinskaya, A. M. Guskov, E. K. Lipatova, M. N. Lavrova   +5 more
doaj   +1 more source

Why Do We Need Alternative Methods for Fungal Disease Management in Plants? [PDF]

Plants (Basel), 2023
McLaughlin MS, Roy M, Abbasi PA, Carisse O, Yurgel SN, Ali S.   +5 more
europepmc   +1 more source

Cell density–dependent nuclear‐cytoplasmic shuttling of SETDB1 integrates with Hippo signaling to regulate YAP1‐mediated transcription

FEBS Letters, EarlyView.
At low cell density, SETDB1 and YAP1 accumulate in the nucleus. As cell density increases, the Hippo pathway is gradually activated, and SETDB1 is associated with increased YAP1 phosphorylation. At high cell density, phosphorylated YAP1 is sequestered in the cytoplasm, while SETDB1 becomes polyubiquitinated and degraded by the ubiquitin–proteasome ...
Jaemin Eom, Jaewoong Jang, Jung Sun Park, Yong‐Kook Kang   +3 more
wiley   +1 more source

A 3D-Printed Dummy for Training Distal Phalanx Amputation in Mice

Animals
The development of realistic dummies for training the distal phalanx amputation (DPA) technique in mouse pups is a promising alternative to reduce and replace animals in training for research and teaching.
Miriam Heuser, Fernando Gonzalez-Uarquin, Maximilian Nuber, Marc A. Brockmann, Jan Baumgart, Nadine Baumgart   +5 more
doaj   +1 more source

Editorial: Advances in alternative methods in preclinical pharmacology and toxicology. [PDF]

Front Pharmacol, 2023
Betti AH, Kenna JG, Van Vleet TR, Aruselvan P, Girardi Müller L.   +4 more
europepmc   +1 more source

Alternative Method to Determine the Amplitude of Limit Cycles of a Class of Nonlinear Electrical Circuit Oscillators

, 2022
Ivo Barbi
openalex   +1 more source

Inhibiting stearoyl‐CoA desaturase suppresses bone metastatic prostate cancer by modulating cellular stress, mTOR signaling, and DNA damage response

FEBS Letters, EarlyView.
Bone metastasis in prostate cancer (PCa) patients is a clinical hurdle due to the poor understanding of the supportive bone microenvironment. Here, we identify stearoyl‐CoA desaturase (SCD) as a tumor‐promoting enzyme and potential therapeutic target in bone metastatic PCa.
Alexis Wilson, Mackenzie K Herroon, Shane Mecca, Laimar C Garmo, Jacob Lindquist, Shrila Rajendran, Steve M. Patrick, Izabela Podgorski   +7 more
wiley   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Alternative Methods for Skin-Sensitization Assessment. [PDF]

Toxics, 2022
Gądarowska D, Kalka J, Daniel-Wójcik A, Mrzyk I.   +3 more
europepmc   +1 more source