Results 231 to 240 of about 344,150 (327)

Structure‐Guided Engineering of a Cas12i Nuclease Unlocks Near‐PAMless Genome Editing

Advanced Science, EarlyView.
CRISPR‐Cas nucleases are limited by PAM requirements, restricting genome accessibility. Structure‐guided engineering of the compact Cas12i nuclease SF01 produced three variants with near‐PAMless, enabling efficient editing at diverse 5'‐NNTN‐3' sites. These nucleases expand the editable portion of the human genome more than fourfold, enabling efficient
Qitong Chen, Hanlin Gou, Chao Xu, Sihan Wang, Huitao Zhang, Minglei Song, Mengge Wang, Xingkun Ji, Xiaofei Wei, Yuanyan Tan, Hehua Quan, Pengyu Luo, Hanyu Shou, Pengpeng Liu, Yafeng Liang, Jian‐Kang Zhu   +15 more
wiley   +1 more source

A single-cell atlas of RNA alternative splicing in the glioma-immune ecosystem. [PDF]

Genome Biol
Song X, Tiek D, Lu M, Yu X, Wu R, Walker M, He Q, Sisbarro D, Hu B, Cheng SY.   +9 more
europepmc   +1 more source

Deep Learning Deepens the Analysis of Alternative Splicing

, 2019
Xudong Zou, Xin Gao, Wei Chen
openalex   +1 more source

Tobacco Smoke Exposure From Prenatal To Adolescent Periods Drives IBD Pathogenesis: Dynamic DNA Methylation Signatures Across Lifespan Stages

Advanced Science, EarlyView.
This study illustrates that maternal smoking during pregnancy (MSDP) is an independent risk factor for IBD in offspring, and DNA methylation serves as a key mechanistic pathway connecting early‐life smoking exposure with IBD risk in offspring. That highlights the urgent need for preventive interventions targeting prospective parents and minors to ...
Han Zhang, Jianhui Zhao, Jie Chen, Xinyi Ma, Siyun Zhou, Alexandra Noble, Rahul Kalla, Judith Wellens, Kun Liu, Evropi Theodoratou, Jack Satsangi, Xue Li   +11 more
wiley   +1 more source

Correction: SF3B4 downregulation restrains lung adenocarcinoma tumorigenesis via 5' alternative splicing of KAT2A. [PDF]

Sci Rep
Qu A, Han B, Hua M, Wang C, Li T.
europepmc   +1 more source

Alphaviral Capsid Proteins Inhibit Stress Granule Assembly via Competitive RNA Binding With G3BP1

Advanced Science, EarlyView.
Stress granules exert antiviral functions. This study illustrates a conserved function of alphaviral capsid proteins in modulating stress granules. Oligomerization mediated by a helical motif coupled with a positively charged intrinsically disordered region (IDR) directly competes with G3BP1 for RNA binding, thereby disrupting G3BP1‐RNA liquid–liquid ...
Yun Zhang, Yi Liu, Zhiying Yao, Haolong Lai, Xiaoxin Chen, Ziqiu Wang, Yiqiong Bao, Tingting Li, Xiaoming Zhou, Xiabin Chen, Peiguo Yang   +10 more
wiley   +1 more source

Early alternative splicing signatures and RBP networks in MASLD progression to cirrhosis. [PDF]

BMC Med Genomics
Chen L, Ma W, Hu Y, Qin Y, Zhao S, Yang J.   +5 more
europepmc   +1 more source

An alternative Pit-1 RNA splicing product reveals modular binding and nonmodular transcriptional activities of the POU-specific domain. [PDF]

, 1993
J W Voss, L Wilson, Simon J. Rhodes, Michael G. Rosenfeld   +3 more
openalex   +1 more source

GPCRs in CAR‐T Cell Immunotherapy: Expanding the Target Landscape and Enhancing Therapeutic Efficacy

Advanced Science, EarlyView.
Chimeric antigen receptor T cell therapy faces dual challenges of target scarcity and an immunosuppressive microenvironment in solid tumors. This review highlights how G protein‐coupled receptors can serve as both novel targets to expand the therapeutic scope and functional modules to enhance CAR‐T cell efficacy.
Zhuoqun Liu, Yuchen Xiao, Yamin Zhao, Lihe Dai, David J.H. Shih, Shikang Liang, Honglei Tian, Liu Liu, Feng Tian, Bing Liu, Lei Chang, Chao Zhang   +11 more
wiley   +1 more source

The circadian clock shapes the Arabidopsis transcriptome by regulating alternative splicing and alternative polyadenylation

, 2020
Yuchen Yang, Yun Li, Aziz Sancar, Onur Oztas   +3 more
openalex   +1 more source