Amber Suppression Technology for Mapping Site-specific Viral-host Protein Interactions in Mammalian Cells [PDF]
Bio-Protocol, 2022 Probing the molecular interactions of viral-host protein complexes to understand pathogenicity is essential in modern virology to help the development of antiviral therapies.
Nur Firdaus Isa +2 more
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A CHO-Based Cell-Free Dual Fluorescence Reporter System for the Straightforward Assessment of Amber Suppression and scFv Functionality [PDF]
Frontiers in Bioengineering and Biotechnology, 2022 Incorporation of noncanonical amino acids (ncAAs) with bioorthogonal reactive groups by amber suppression allows the generation of synthetic proteins with desired novel properties.
Simon K. Krebs +8 more
doaj +2 more sources
South Brazilian Amber Propolis: Chemical Profile, and Antioxidant, Antimicrobial and Antileukemic Activities [PDF]
MoleculesThis study introduces “amber propolis,” a unique variety from the Pampa biome in southern Brazil, characterized by a distinct chemical fingerprint and high biological potency.
Viviane Ulbrich-Ferreira +11 more
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Protocol for click labeling of HIV-1 envelope on amber-free virions prepared using genetic code expansion [PDF]
STAR ProtocolsSummary: Site-specific fluorescent labeling of HIV-1 proteins enables direct visualization of their dynamics and trafficking. Here, we present a protocol to label HIV-1 envelope (Env) glycoproteins on virions using genetic code expansion and click ...
Wang Xu +2 more
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Genomically integrated orthogonal translation system in Escherichia coli enables production of functional modified [NiFe]-hydrogenases [PDF]
Microbial Cell FactoriesThe functional diversification of O2-tolerant [NiFe]-hydrogenases using orthogonal translation systems (OTSs) offers a promising strategy for developing advanced biocatalysts and biohybrid energy platforms.
Qin Fan +5 more
doaj +2 more sources
Genetic Code Expanded T Cell for Controllable Immunotherapy [PDF]
Advanced ScienceChimeric antigen receptor (CAR)‐T cell therapy has demonstrated curative potential against hematologic malignancies, but its clinical application remains constrained by the risk of uncontrolled immune activation.
Xue Wang +4 more
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Amber suppression coupled with inducible surface display identifies cells with high recombinant protein productivity [PDF]
Biotechnology and Bioengineering, 2019 Marcello Marelli +2 more
exaly +2 more sources
A Genetically Encoded Picolyl Azide for Improved Live Cell Copper Click Labeling
Frontiers in Chemistry, 2021 Bioorthogonal chemistry allows rapid and highly selective reactivity in biological environments. The copper-catalyzed azide–alkyne cycloaddition (CuAAC) is a classic bioorthogonal reaction routinely used to modify azides or alkynes that have been ...
Birthe Meineke +9 more
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Neurobiology of Sleep and Circadian Rhythms, 2021 The magnitude of the stimulus to the biological clock will depend upon the distribution of circadian phototransduction circuits across the retinae and the spatial distribution of luminous stimuli in the environment.
Mark S. Rea +2 more
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Nonsense and sense suppression abilities of original and derivative Methanosarcina mazei pyrrolysyl-tRNA synthetase-tRNA(Pyl) pairs in the Escherichia coli BL21(DE3) cell strain. [PDF]
PLoS ONE, 2013 Systematic studies of nonsense and sense suppression of the original and three derivative Methanosarcina mazei PylRS-tRNA(Pyl) pairs and cross recognition between nonsense codons and various tRNA(Pyl) anticodons in the Escherichia coli BL21(DE3) cell ...
Keturah A Odoi +3 more
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